ANTILISTERIAL ACTYVITY OF LACTIC ACID BACTERIA ISOLATED FROM GILTHEAD BREAMS AND SEA BASSES FILLETS PACKAGED MAP AGAINST PRIMITIVE STRAINS OF LISTERIA MONOCYTOGENES

  • M. Barile | lucia.zoppi@pagepress.org Dipartimento di Scienze Zootecniche ed Ispezione degli Alimenti di Origine Animale, Facoltà di Medicina Veterinaria, Via Delpino 1, 80137 Napoli, Italy.
  • A. Mormile Dipartimento di Scienze Zootecniche ed Ispezione degli Alimenti di Origine Animale, Facoltà di Medicina Veterinaria, Via Delpino 1, 80137 Napoli, Italy.
  • R. Mercogliano Dipartimento di Scienze Zootecniche ed Ispezione degli Alimenti di Origine Animale, Facoltà di Medicina Veterinaria, Via Delpino 1, 80137 Napoli, Italy.
  • N. Murru Dipartimento di Scienze Zootecniche ed Ispezione degli Alimenti di Origine Animale, Facoltà di Medicina Veterinaria, Via Delpino 1, 80137 Napoli, Italy.

Abstract

Listeria monocytogenes is the causative agent of listeriosis typically caused by ready-to-eat processed food that have a refrigerated shelf-life, but lightly preserved fish products also belong to a high-risk category. Aim of the work was to evaluate antimicrobial activity linked bacteriocin-producing of LAB isolated from gilthead breams and sea basses fillets packaged in modified atmospheres. Fifty-five LAB strains were screened against 21 strains of Listeria monocytogenes, 1 Listeria innocua held in the culture collection of Department of Zootechnical Sciences and Food Ispection (SIA) and submitted to antagonistic activity using the spot on lawn and the agar well diffusion assay. Lactococcus lactis sub. lactis Sa31 was able to produce bacteriocin in agar and different broth medium. The bacteriocin man31 showed sensitivity to trypsin, pronase E and papain, inactivation at temperatures ≥ 100°C, bactericidal mode of action and antilisterial act, rapidly. The bacteriocin man31 caused a reduction of L. monocytogenes ½ c growth about log10 > 3 UFC/ml, when was applied on indicator strain at 20,480 AU/ml concentration, in vitro.

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Published
2011-04-01
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Original Articles
Keywords:
Lactic Acid bacteria, bacteriocin, Listeria monocytogenes
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How to Cite
1.
Barile M, Mormile A, Mercogliano R, Murru N. ANTILISTERIAL ACTYVITY OF LACTIC ACID BACTERIA ISOLATED FROM GILTHEAD BREAMS AND SEA BASSES FILLETS PACKAGED MAP AGAINST PRIMITIVE STRAINS OF LISTERIA MONOCYTOGENES. Ital J Food Safety [Internet]. 2011Apr.1 [cited 2021May16];1(1):23-8. Available from: https://www.pagepressjournals.org/index.php/ijfs/article/view/ijfs.2011.1.23