Quantitative determination of artificial sweeteners and sucrose in energy drinks and mango juice available in Dhaka city


Submitted: 6 October 2022
Accepted: 14 December 2023
Published: 25 January 2024
Abstract Views: 968
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Authors

Energy drinks and mango juice are popular beverages. Apart from the natural ingredients and some additives present in these drinks, sugar is an important component of both. It has been established that, other than providing sweetness, sugars are potent to bring about health consequences for their consumers. Sweeteners, both artificial (aspartame, sodium cyclamate, and saccharin) and natural (sucrose), were our centers of interest. This study aimed to determine the presence and levels of these sweeteners in energy drinks and mango juice. Spectrophotometric methods were used to determine the concentration of the mentioned sugars. For this purpose, a total of 42 samples of 7 different brands were collected from different locations in Dhaka city, Bangladesh. The methods were found to be linear over the concentration range of 10-26 µg/mL (r2=0.9989), 137-320 µg/mL (r2=0.9891), 2.5-24 µg/mL (r2=0.9915) and 2354-2784 µg/mL (r2=0.9985) for aspartame, sodium cyclamate, saccharin, and sucrose, respectively. Mango juice contained a relatively lower amount of saccharin compared to energy drinks. In the case of aspartame, one brand of energy drinks had the least amount. Moreover, both energy drinks and mango juice had a similar content of sodium cyclamate, but one brand of mango juice had a relatively low content of sodium cyclamate.


Arnold DL, Moodie CA, Grice MC, Charbonneau SM, Stavric B, Collins BT, McGuire PF, Zawidzka ZZ, Munro LC, 1980. Long-term toxicity of ortho-toluenesulfonamide and sodium-saccharin in the rat. Toxicol Appl Pharmacol 52:113-52.

Bhupathiraju S, Pan A, Malik V, Manson J, Willett W, van Dam R, Hu F, 2013. Caffeinated and caffeine-free beverages and risk of type 2 diabetes. Am J Clin Nutr 97:155-66.

Celik E, Demirhan BE, Demirhan B, Yentur G, 2014. Determination of aspartame levels in soft drinks consumed in Ankara, Turkey. J Food Res 3:156-9.

Duffey KJ, Steffen LM, Van Horn L, Jacobs DR, Popkin BM, 2012. Dietary patterns matter: diet beverages and cardiometabolic risks in the longitudinal coronary artery risk development in young adults (CARDIA) study. Am J Clin Nutr 95:909-15.

Fowler SP, Williams K, Resendez RG, Hunt KJ, Hazuda HP, Stern MP, 2008. Fueling the obesity epidemic? Artificially sweetened beverage use and long-term weight gain. Obesity 16:1894-900.

Gardner C, Wylie-Rosett J, Gidding SS, Steffen LM, Johnson RK, Reader D, Lichtenstein AH, 2012. Non-nutritive sweeteners: current use and health prospectives. Circulation 126:509-19.

Gimba CE, Abechi SE, Abbas NS, Gerald IU, 2014. Evaluation of caffeine, aspartame and sugar content in energy drinks. J Chem Paharm Res 6:39-43.

Hashemi M, Zohrabi P, Abdolhosseini S, 2015. Spectrophotometric determination of cyclamate in artificial sweeteners and beverages after ultrasound-assisted emulsification microextraction. Anal Methods 7:2594-602.

Kojima S, Ichibagase H, 1966. Studies on synthetic sweetening agents. 8. Cyclohexylamine, a metabolite of sodium cyclamate. Chem Pharm Bull 14:971-4.

Lau OW, Luk SF, Chan WM, 1988. Spectrophotometric determination of aspartame in soft drinks with ninhydrin as reagent. Analyst 113:765-8.

Mathew SB, Pillai AK, Gupta VK, 2006. Spectrophotometric method for the determination of saccharin in food and pharmaceutical products. Indian J Pharm Sci 68:821-3.

Medline Plus, 2017. Phenylketonuria. Available from: https://ghr.nlm.nih.gov/condition/phenylketonuria.

Mortensen A, 2006. Sweeteners permitted in the European Union: safety aspects. Scandinavian J Food Nutr 50:104-16.

Price JM, Biava CG, Oser BL, Vogin EE, Steinfeld J, Ley HL, 1970. Bladder tumors in rats fed cyclohexylamine or high doses of a mixture of cyclamate and saccharin. Science 167:1131-2.

Ramsurn D, Jhaumeer Lauloo S, Bhowon MG, 2015. Determination of artificial sweeteners in liquid foods by high performance chromatography. Int J Pharm Drug Anal 3:311-21.

Reid JL, McCrory C, White CM, Martineau C, Vanderkooy P, Fenton N, Hammond D, 2017. Consumption of caffeinated energy drinks among youth and young adults in Canada. Prev Med Rep 5:65-70.

Sardesai VM, Waldshan TH, 1991. Natural and synthetic intense sweeteners. J Nutr Biochem 2:236-44.

Schernhammer ES, Bertrand KA, Birmann BM, Sampson L, Willet WC, Feskanich D, 2012. Consumption of artificial sweetener and sugar-containing soda and risk of lymphoma and leukemia in men and women. Am J Clin Nutr 96:1419-28.

Serdar M, Knezevic Z, 2011. Determination of artificial sweeteners in beverages and special nutritional products using high performance liquid chromatography. Arh Hig Rada Toksikol 62:169-73.

Singh M, Kumar A, Tarannum N, 2013. Water-compatible ‘aspartame’- imprinted polymer grafted on silica surface for selective recognition in aqueous solution. Anal Bioanal Chem 405:4245-52.

Suez J, Korem T, Zeevi D, Zilberman-Schapira G, Thaiss C, Maza O, Israeli D, Zmora N, Gilad S, Weinberger A, Kuperman Y, Harmelin A, Kolodkin-Gal I, Shapiro H, Halpern Z, Segal E, Elinav E, 2014. Artificial sweeteners induce glucose intolerance by altering the gut microbiota. Nature 514:181-6.

Swithers SE, 2013. Artificial sweeteners produce the counterintuitive effect of inducing metabolic derangements. Trends Endocrinol Metab 24:431- 41.

1.
Islam K, Tehjib Meghla Y, Akhtaruzzaman M, Shamsur Rouf AS, Hossian MS, Hossain M. Quantitative determination of artificial sweeteners and sucrose in energy drinks and mango juice available in Dhaka city. Ital J Food Safety [Internet]. 2024 Jan. 25 [cited 2024 Apr. 16];13(1). Available from: https://www.pagepressjournals.org/ijfs/article/view/10914

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