Distribution of Pseudomonas species in a dairy plant affected by occasional blue discoloration

Submitted: 16 May 2013
Accepted: 17 October 2014
Published: 10 December 2014
Abstract Views: 2311
HTML: 250
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.


During 2010 many cases of discoloration in mozzarella, popularly termed as blue mozzarella, have been reported to the attention of public opinion. Causes of the alteration were bacteria belonging to the genus Pseudomonas. The strong media impact of such cases has created confusion, not only among consumers, but also among experts. In order to help improving the knowledge on microbial ecology of this microorganism a study has been set up with the collaboration of a medium-sized dairy plant producing fresh mozzarella cheese, with occasional blue discoloration, conducting surveys and sampling in the pre-operational, operational and post-operational process phase, milk before and after pasteurization, water (n=12), environmental surfaces (n=22) and the air (n=27). A shelf life test was conducted on finished products stored at different temperatures (4-8°C). Among the isolates obtained from the microbiological analysis of the samples, 60 were subjected to biomolecular tests in order to confirm the belonging to Pseudomonas genus and to get an identification at species level by the amplification and sequencing of the gyrB gene. The results of microbiological tests demonstrated the presence of microorganisms belonging to the genus Pseudomonas along the entire production lane; molecular tests showed 7 different species among the 40 isolates identified. One particular species (Pseudomonas koreensis) was isolated from blue discolored mozzarella cheese and was indicated as the most relevant for the production plant, both for the distribution along the processing chain and for the consequences on the finished product.

Chiesa F, Lomonaco S, Nucera D, Garoglio D, Dalmasso A, Civera T. Distribution of Pseudomonas species in a dairy plant affected by occasional blue discoloration. Ital J Food Safety [Internet]. 2014 Dec. 10 [cited 2024 Jun. 13];3(4). Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2014.1722


Download data is not yet available.


Similar Articles

You may also start an advanced similarity search for this article.