Original Articles
AIVI - December 2009 - Issue 6
https://doi.org/10.4081/ijfs.2009.1125

DEVELOPMENT OF A PCR-RFLP METOD FOR THE IDENTIFICATION OF SIX SPECIES BELONGING TO THE GENUS LOPHIUS

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Received: 13 February 2013
Published: 13 December 2009
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Fish species identification, Lophius, PCR-RFLP

Authors

A. Armani
lucia.zoppi@pagepress.org
Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, .
L. Castigliego
Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, .
A. Guidi
Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, .
G. Gandini
Ministero della Salute, PIF Bologna, .
P. Manzoni
, .
F. Susini
Istituto Zooprofilattico Lazio e Toscana, laboratorio di Ittiopatologia, .
D. Gianfaldoni
Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, .
Nowadays, six of the seven species belonging to the Genus Lophius have an important commercial value in the national and international markets. Usually they are sold beheaded and for this reason they are called tails. This kind of preparation is a limit for the specie-identification by means of the morphological characteristics. The mitochondrial cytochrome b (Cyt b) gene is considered a useful genetic marker to identify fish species. In this work, after obtaining the Cyt b complete sequence of the Lophius species that were missed in the databases, we set up a method based on PCR-RFLP able to identify the six species of Lophius with a commercial denomination in the Italian market.

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1.
DEVELOPMENT OF A PCR-RFLP METOD FOR THE IDENTIFICATION OF SIX SPECIES BELONGING TO THE GENUS LOPHIUS. Ital J Food Safety [Internet]. 2009 Dec. 13 [cited 2026 Sep. 14];1(6):63-7. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2009.6.63

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