Identification and serotyping of Listeria monocytogenes, isolated from various salmon products, sold in retail market in Lithuania

Submitted: 8 September 2020
Accepted: 6 July 2021
Published: 29 September 2021
Abstract Views: 629
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Cold smoked salmon products (belly flaps, pieces, fillet, and loin) obtained from the retail market in Lithuania were tested for the presence of L. monocytogenes. It was found that contamination of the cold smoked fish products with Listeria spp. depends on the type of the product. Contamination with listeria in salmon belly flaps was 7.5 times higher than in the loin (P<0.05), 1.8 times higher than in the pieces (P<0.05) and 30 times higher than in the fillet (P<0.05). Microbiological analysis showed that 32.5% (P<0.05) of the fish product samples were infected with L. monocytogenes, while multiplex PCR confirmed 31.25% positive samples (P<0.01). According to the study results, L. monocytogenes strains were divided into two serotypes: 4b (94.6%) and 1/2a (5.4%). High contamination of the products with Listeria spp. showed that cold smoked salmon products, sold in local market, can be a reason of human listeriosis in Lithuania.

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Citations

1. Atil E, Ertas HB, Ozbey G, 2011. Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples. Vet Med 56(8):386–394.
2. Bubert A, Hein I, Rauch M, Lehner A, Yoon B, Goebel W, Wagner M, 1999. Detection and Differentiation of Listeria spp. by a Single Reaction Based on Multiplex PCR Applied and Environmental Microbiology. Appl Environ Microbiol 65(10):4688–4692.
3. Doumith M, Buchrieser C, Glaser P, Jacquet C., Martin P, 2004. Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCR Journal of clinical microbiology. J Clin Microbiol 42(8): 3819–3822.
4. Jeyaletchumi P, Tunung R, Margaret SP, Son R, Farinazleen MG, Cheah YK, 2010. Detection of Listeria monocytogenes in foods. Int Food Res J 17:1–11.
5. Garrido V, Vitas AI, García-Jalón I, 2010. Current Research, Technology and Education Topics in Appplied Microbiology and Microbial Biotechnology. Chapter: The problem of Listeriosis and ready-to eat products: prevalence and persistence. Editors: Méndez-Vilas A. 1182–1189.
6. Gombas DE, Chen Y, Clavero RS, Scott VN, 2003. Survey of Listeria monocytogenes in ready-to-eat foods. J Food Prot 66:559–569.
7. Liu D, 2006. Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Med Microbiol 55:645–659.
8. Miettinen H, Wirtanen G, 2006. Ecology of Listeria spp. in a fish and molecular typing of Listeria monocytogenes from fish farming and procesing companies. Int J of Food Microbiol 112:138–146.
9. McLauchlin J, Mitchell RT, Smerdon WJ, Jewell K, 2004. Listeria monocytogenes and listeriosis: a review of hazard characterization for use in microbiological risk assessment of foods. Int J Food Microbiol 92:15–33.
10. Mesak LR, Kovačević J, Kevin JA, 2012. Occurrence and characterization of Listeria spp. in ready-to-eat retail foods from Vancouver, British Columbia. Food Microbiol 30:372–378.
11. Nissreen AG, Sapna CD, Enda JC, 2011. Prevalence and typing of Listeria monocytogenes strains in retail vacuum – packed cold - smoked salmon in the Republic of Ireland. J Food Saf 31(1):21–45.
12. EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control), 2016. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2015. EFSA J 13(1):3991.
13. EFSA and ECDC (European Food Safety Authority and European Centre for Disease Prevention and Control), 2019. The European Union One Health 2018 Zoonoses Report. EFSA
J 2019;17(12):5926, 276 pp.
14. Dhama K, Karthik K, Tiwari R, Shabbir MZ, Barbuddhe S, Malik SVS, Singh RK, 2015. Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: A comprehensive review. Vet Q 35:211–235.
15. Sauders BD, Pettit D, Currie B, Suits P, Evans A, Stellrecht K, Dryja DM, Slate D, Wiedmann M, 2005. Low prevalence of Listeria monocytogenes in human stool. J Food Prot 68(1):178–81.
16. Braga V, Vázquez S, Vico V, Pastorino V, Mota MI, Legnani M, Schelotto F, Lancibidad G, Varela G, 2017. Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay. Braz J Microbiol 48(4):689–694.
17. Lopez-Valladares G, Danielsson-Tham ML, Tham W, 2018. Implicated Food Products for Listeriosis and Changes in Serovars of Listeria monocytogenes Affecting Humans in Recent Decades. Foodborne Pathog Dis 15(7):387–397.
18. Zhang Y, Dong S, Chen H, Chen J, Zhang J, Zhang Z, Yang Y, Xu Z, Zhan L, Mei L, 2019. Prevalence, Genotypic Characteristics and Antibiotic Resistance of Listeria monocytogenes From Retail Foods in Bulk in Zhejiang Province, China. Front Microbiol. 25(10):1710.
19. Narkevičius R, 2004. Šaltai rūkytos žuvies saugos užtikrinimo problemos. Maisto chemija ir technologija 38(2):29–34.
20. Centre for Communicable Diseases and AIDS. http://www.ulac.lt/en/naujienos/press-releases/in-july-two-people-died-in-lithuania-from-listeriosis, logged in on 5th of September, 2020.

How to Cite

1.
Simonavičienė I, Zakariene G, Lozoraitytė A, Zaborskienė G, Gerulis G, Stimbirys A. Identification and serotyping of <em>Listeria monocytogenes</em>, isolated from various salmon products, sold in retail market in Lithuania. Ital J Food Safety [Internet]. 2021 Sep. 29 [cited 2024 Jul. 17];10(3). Available from: https://www.pagepressjournals.org/ijfs/article/view/9341

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