Food safety knowledge, hygiene practices among food handlers, and microbiological quality of animal side dishes in contract catering

Submitted: 9 April 2024
Accepted: 13 May 2024
Published: 21 May 2024
Abstract Views: 758
PDF: 1522
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Food poisoning is a major concern in catering services in Indonesia, with multiple outbreaks being reported in factories and offices. This study aimed to assess the level of food safety knowledge, personal hygiene practices, and microbiological quality of chicken and egg dishes at three catering companies in the Yogyakarta Special Region of Indonesia, which provide mass catering for workers. From March to July 2019, a cross-sectional study was conducted using validated questionnaires, observation checklists, and microbiological tests to measure the parameters of Escherichia coli and Staphylococcus aureus. Pearson and Rank-Spearman correlation tests, as well as independent sample t-tests, were used for statistical analysis. The study revealed that 75% of the subjects had limited knowledge of food safety, with only 5% having a good understanding. Furthermore, 95% of the subjects had not received any food safety training, resulting in poor hygiene practices among the food handlers. Although there was no significant correlation between food safety knowledge and hygiene practices (p>0.05), certain practices, such as appearance and hand washing, were significantly correlated with age. Employment duration was negatively correlated with food safety knowledge (p=0.027; r=-0.494). While chicken samples were free from E. coli and S. aureus, one egg sample was found to be contaminated with E. coli. The research emphasizes the significance of providing frequent food safety training to food handlers, ideally every year. Additionally, it recommends conducting larger sample studies and collaborating with government agencies for future research.



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Abdul-Mutalib N, Abdul-Rashid MF, Mustafa S, Amin-Nordin S, Hamat RA, Osman M, 2012. Knowledge, attitude and practices regarding food hygiene and sanitation of food handlers in Kuala Pilah, Malaysia. Food Control 27:289-93. DOI:
Arikunto S, 2013. Prosedur penelitian: suatu pendekatan praktik. PT Rineka Cipta, Jakarta, Indonesia. [Book in Indonesian].
Arisanti RR, Indriani C, Wilopo SA, 2018. Contribution of agents and factors causing foodborne outbreak in Indonesia: a systematic review. Berita Kedokteran Masyarakat (BKM Journal of Community Medicine and Public Health) 34:99-106. [Article in Indonesian]. DOI:
Bellio A, Gallina S, Traversa A, Monfardini S, Bianchi DM, Adriano D, Decastelli L, 2012. Microbiological criteria in public catering: sampling and auditing experiences in canteens and cafeterias in Piedmont. Ital J Food Saf 1:11-6. DOI:
Blikon MOE, Rahayu T, Rahmawati A, 2017. Penerapan Hazard Analysis Critical Control Point (HACCP) pada usaha jasaboga di Kecamatan Kotagede, Yogyakarta. Kingdom (The Journal of Biological Studies) 6:343-9. [Article in Indonesian]. DOI:
Disanto C, Celano G, Dambrosio A, Quaglia NC, Bozzo G, Tritto A, Celano GV, 2020. Food safety in collective catering: knowledge, attitudes and correct application of GHP/GMP knowledge among foodservice workers. Ital J Food Saf 9:8453. DOI:
Doğan M, Tekiner İH, 2021. On-site and one-year monitoring of food service business risks associated with poor hygiene quality in the catering establishments for consumer protection. J Foodserv Bus Res 24:274-85. DOI:
European Food Safety Authority, 2018. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2017. EFSA J 16:e05500. DOI:
Fariba R, Gholamreza JK, Saharnaz N, Ehsan H, Masoud Y, 2018. Knowledge, attitude, and practice among food handlers of semi-industrial catering: a cross sectional study at one of the governmental organization in Tehran. J Environl Health Sci Eng 16:249-56. DOI:
Fatimah S, Hekmah N, Fathullah DM, Norhasanah N, 2022. Cemaran mikrobiologi pada makanan, alat makan, air dan kesehatan penjamah makanan di Unit Instalasi Gizi Rumah Sakit X di Banjarmasin. J Nutr Coll 11:322-7. [Article in Indonesian]. DOI:
Firdani F, 2022. Knowledge, attitudes and practices of hygiene and sanitation implementation on food handlers. Jurnal Kesehatan Lingkungan Indonesia 21:131-6. [Article in Indonesian]. DOI:
Garayoa R, Vitas AI, Díez-Leturia M, García-Jalón I, 2011. Food safety and the contract catering companies: Food handlers, facilities and HACCP evaluation. Food Control 22:2006-12. DOI:
Hanifi AS, 2019. Analisis perilaku higiene individu penjamah makanan, HACCP, dan cemaran mikrobiologi pada menu makanan berbahan dasar telur di katering wilayah Sleman dan Yogyakarta. Universitas Gadjah Mada, Yogyakarta, Indonesia. [Material in Indonesian].
Indonesia National Agency of Drug and Food Control, 2012. Keamanan Pangan di Kantin Sekolah. Direktorat Surveilan dan Penyuluh Keamanan Pangan, Jakarta, Indonesia. [Material in Indonesian].
Indonesia National Agency of Drug and Food Control, 2019. Food and Drug Supervisory Agency Regulation number 13 of 2019 concerning maximum limits for microbial contamination in processed food. [Material in Indonesian].
Indonesia National Agency of Drug and Food Control, 2020. 2019 Annual report of the center for food and drug data and information. Badan Pengawas Obat dan Makanan, Jakarta, Indonesia. [Material in Indonesian].
Ismail FH, Chik CT, Muhammad R, Yusoff NM, 2016. Food safety knowledge and personal hygiene practices amongst mobile food handlers in Shah Alam, Selangor. Procedia Soc Behav Sci 222:290-8. DOI:
Khairina AD, Palupi IR, Prawiningdyah Y, 2018. Pengaruh media visual higiene sanitasi makanan terhadap praktik higiene penjamah makanan di Kantin Kampus. J Health Educ 3:65-74. [Article in Indonesian].
Khoirunnisa FA, 2019. Analisis tingkat pengetahuan keamanan pangan penjamah makanan, penerapan HACCP, dan uji mikrobiologis menu makanan berbahan dasar ayam di jasa boga Yogyakarta. Universitas Gadjah Mada, Yogyakarta, Indonesia. [Material in Indonesian].
Kim JG, 2019. Gender and age differences in hand hygiene practices among the general population. J Environ Health Sci 45:213-21. [Article in Korean].
Ko WH, 2010. Factors affecting the foodservice satisfaction of food contractor operations - a case study of an employee cafeteria in a high-tech manufacturing plant in Taiwan. J Culin Sci Technol 8:158-65. DOI:
Ko WH, 2013. The relationship among food safety knowledge, attitudes and self-reported HACCP practices in restaurant employees. Food Control 29:192-7. DOI:
Kubde SR, Pattankar J, Kokiwar PR, 2016. Knowledge and food hygiene practices among food handlers in food establishments. Int J Community Med Public Health 3:251-6. DOI:
Maharani CF, Lestari F, Sari RP, Ristandi S, 2018. Community empowerment program in Bojong Terong, Depok, West Java: established a healthy catering business that takes into account the industrial hygiene aspects. KnE Life Sci 4:71-8. DOI:
Marfuah D, Wardana AS, Naresti F, 2023. Food sanitation hygiene education in Increasing Knowledge and Behavior of Food Handlers at Alhikmah Catering Surakarta. Prosiding University Research Colloquium 38-51.
Ministry of Health - Republic of Indonesia, 2011. Regulation of the Minister of Health of the Republic of Indonesia number 1096/MENKES/PER/VI/2011 concerning hygiene sanitation food services. [Material in Indonesian].
Ministry of Health - Republic of Indonesia, 2012. Buku panduan penyelenggaraan hari cuci tangan pakai sabun sedunia (HCTPS). Pusat Komunikasi Publik Kementerian Kesehatan, Jakarta, Indonesia. [Material in Indonesian].
Ministry of Health - Republic of Indonesia, 2013. Regulation of the Minister of Health of the Republic of Indonesia number 2 of 2013 concerning extraordinary events of food poisoning. [Material in Indonesian].
Mullan BA, Wong CL, 2009. Hygienic food handling behaviours. An application of the theory of planned behaviour. Appetite 52:757-61. DOI:
Nursalam, 2008. Konsep dan penerapan metodologi penelitian ilmu keperawatan, pedoman skripsi, tesis, dan instrumen penelitian keperawatan edisi 2. Salemba Medika, Jakarta, Indonesia. [Book in Indonesian].
Osimani A, Aquilanti L, Clementi F. 2016. Salmonellosis associated with mass catering: a survey of European Union cases over a 15-year period. Epidemiol Infect 144:3000-12. DOI:
Prihastuti R, Depo M, Wibowo TA, Misinem, 2018. Catered lunch suspected outbreak in garment factory, Sleman District, Yogyakarta, Indonesia, 2017. Int J Health Med Eng 12.
Putri MS, Susanna D, 2021. Food safety knowledge, attitudes, and practices of food handlers at kitchen premises in the port ‘X’area, North Jakarta, Indonesia 2018. Ital J Food Saf 10:9215. DOI:
Rahmadhani D, Sumarmi S, 2017. The description of food sanitation and hygiene at PT Aerofood Indonesia, Tangerang, Banten. Amerta Nutrition 1:291-9. [Article in Indonesian]. DOI:
Rahmayani RD, Simatupang MM, 2019. Analisis pengaruh higiene penjamah dan sanitasi makanan terhadap kontaminasi E. coli pada jajanan sekolah. JUKMAS 3:164-78. [Article in Indonesian].
Renn RW, Preston F, Fabian F, Steinbauer R. 2024. Employee work habits: a definition and process model. Hum Resour Manag Rev 34:101009. DOI:
Rivani SK, Putriningtyas ND, 2023. Correlation of knowledge and attitude of food handlers with the implementation of sanitation hygiene in Enthong Mas Catering. Darussalam Nutrition Journal 7:53-64. [Article in Indonesian]. DOI:
Rohmalia PA, Djajalaksana YM, 2013. Pengelolaan Bisnis Catering dengan Memanfaatkan Sistem Informasi Berbasis Web (Studi Kasus pada Anggun Catering). Jurnal Teknik Informatika dan Sistem Informasi 8:219812. [Article in Indonesian].
Romanda F, Priyambodo, Arisanti ED, 2016. Hubungan personal hygiene dengan keberadaan escherichia coli pada makanan di tempat pengolahan makanan (TPM) buffer area Bandara Adi Soemarmo Surakarta. Biomedika 8:41-6. [Article in Indonesian]. DOI:
Salsabela FL, Abryanto R, 2022. Analysis of food handler’s knowledge of hygiene and sanitation impact on food quality. Adv Biol Sci Res 16:310-4. DOI:
Siau AMF, Son R, Mohhiddin O, Toh PS, Chai LC, 2015. Food court hygiene assessment and food safety knowledge, attitudes and practices of food handlers in Putrajaya. Int Food Res J 22:1843-54.
Sihombing J, Padmawati RS, Kristina SA, 2018. Knowledge, attitude, and practices regarding food safety among food employees in Ambon City, Indonesia. Mal J Nutr 24:293-9.
Smigic N, Djekic I, Martins ML, Rocha A, Sidiropoulou N, Kalogianni EP, 2016. The level of food safety knowledge in food establishments in three European countries. Food Control 63:187-94. DOI:
Sneed J, Strohbehn C, Gilmore SA, Mendonca A, 2004. Microbiological evaluation of foodservice contact surfaces in Iowa assisted-living facilities. J Am Diet Assoc 104:1722-4. DOI:
Soedarto, 2015. Medical Microbiology. Sagung Seto, Jakarta, Indonesia.
Sunaryo S, 2011. Hubungan pengetahuan dengan sikap dan tindakan penjamah makanan dalam aspek keamanan pangan pada usaha rumah makan berdasarkan Skor Keamanan Pangan (SKP) di Pantai Kuwaru Kabupaten Bantul. Universitas Gadjah Mada, Yogyakarta, Indonesia. [Material in Indonesian].
Suryani D, Sutomo AH, Aman AT, 2019. The factors associated with food safety practices on food handlers in primary school canteens. Unnes J Public Health 8:1-9. DOI:
Susanna D, Eryando T, Kusuma A, 2015. The relationship between knowledge and behaviour of food handlers to Escherichia coli contamination in serving foods in a campus. World Appl Sci J 33:1125-31.
Wiariyanti WR, Lalu RV, Wibowo TA, Prasetyaningsih EC, 2018. Food poisoning in office X, Sleman District, DIY Province, Indonesia - 2018. Berita Kedokteran Masyarakat 34:1-7.
Wulandari A, Rahayu T, 2018. Higiene dan sanitasi usaha jasaboga di Seturan, Kecamatan Depok, Kelurahan Caturtunggal, DI Yogyakarta. Kingdom (The Journal of Biological Studies) 7:628-35. [Article in Indonesian]. DOI:
Yardımcı H, Hakli G, Çakiroğlu FP, Özçelik AÖ, 2015. Hygiene knowledge of food staff in catering industry: a sample from Turkey. SAGE Open 5:2158244015580376. DOI:
Yulistiani R, 2010. Study of un-slaughtered chicken carcass: organoleptic changes and bacterial growth pattern. Jurnal Teknologi Pertanian 11:27-36.
Zaitun, Siagian A, Julianti E, Setianingsih RM, 2018. The environmentally sound management model for catering service. IJCIET 9:666-80.
Zhang Y, Xu D, Shi L, Cai R, Li C, Yan H, 2018. Association between agr type, virulence factors, biofilm formation and antibiotic resistance of Staphylococcus aureus isolates from pork production. Front Microbiol 9:1876. DOI:

How to Cite

Palupi IR, Budiningsari RD, Khoirunnisa FA, Hanifi AS. Food safety knowledge, hygiene practices among food handlers, and microbiological quality of animal side dishes in contract catering. Ital J Food Safety [Internet]. 2024 May 21 [cited 2024 Jul. 25];. Available from: