https://doi.org/10.4081/ijfs.2025.13384
Identification of Actinobacillus seminis as the cause of abortion in sheep by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and whole genome sequencing
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Published: 27 February 2025
Actinobacillus seminis is a gram-negative bacterium that affects reproductive organs, causing epididymitis, low fertility, and occasional abortions in sheep and goats. The virulence factors and the pathogenicity mechanisms of A. seminis have not been clearly elucidated yet. The aim of this study is to report a laboratory investigation performed on a sheep farm in the Basilicata region (southern Italy) following cases of abortion. Four samples of aborted fetuses taken from two sheep were analyzed to investigate the main pathogens causing abortions by bacterial isolation. In addition, serum samples belonging to the sheep have also been analyzed. Analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) of the isolated colonies identified protein spectra referable to A. seminis. This result was also confirmed through the use of genome sequencing. Therefore, these data could explain cases of abortion that occurred on the farm and would confirm that MALDI-TOF MS technology is a rapid, accurate, and inexpensive method for identifying bacteria.
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Al-Katib WA, Dennis SM, 2009. Ovine genital actinobacillosis: a review. N Z Vet J 57:3528. DOI: https://doi.org/10.1080/00480169.2009.64722
Castellana S, De Laurentiis V, Bianco A, Del Sambro L, Grassi M, De Leonardis F, Derobertis AM, De Carlo C, Sparapano E, Mosca A, Stolfa S, Ronga L, Santacroce L, Chironna M, Parisi M, Capozzi L, Parisi A, 2024. Pannonibacter anstelovis sp. nov. isolated from two cases of bloodstream infections in paediatric patients. Microorganisms 12:799. DOI: https://doi.org/10.3390/microorganisms12040799
dos Santos FA, de Azevedo EO, de Azevedo SS, Garino Júnior F, Aparecido Mota R, de Cássia Peixoto Kim P, Vale Gomes AL, Alves CJ, 2014. Isolation of Actinobacillus seminis from a goat with clinical epididymo-orchitis in Brazil. Braz J Microbiol 45:205-8. DOI: https://doi.org/10.1590/S1517-83822014005000024
Knijn A, Michelacci V, Orsini M, Morabito S, 2020. Advanced research infrastructure for experimentation in genomics (ARIES): a lustrum of galaxy experience. Available from: https://www.biorxiv.org/content/10.1101/2020.05.14.095901v1. DOI: https://doi.org/10.1101/2020.05.14.095901
Manzulli V, Rondinone V, Buchicchio A, Serrecchia L, Cipolletta D, Fasanella A, Parisi A, Difato L, Iatarola M, Aceti A, Poppa E, Tolve F, Pace L, Petruzzi F, Della Rovere I, Raele DA, Del Sambro L, Giangrossi L, Galante D, 2021. Discrimination of Bacillus cereus group members by MALDI-TOF mass spectrometry. Microorganisms 9:1202. DOI: https://doi.org/10.3390/microorganisms9061202
Manzulli V, Rondinone V, Serrecchia L, Petrella A, Scaltrito D, Marino L, Pace L, Prencipe ML, Cipolletta D, Nitti M, Fasanella A, Poppa E, Galante D, 2022. Evaluation of a real-time PCR in combination with a cultivation method for the detection of Brucella Abortus in organs of infected cattle in southern Italy. J Vet Res 66:559-63. DOI: https://doi.org/10.2478/jvetres-2022-0062
Moustacas VS, Silva TM, Costa LF, Xavier MN, Carvalho CA Jr, Costa EA, Paixão TA, Santos RL, 2013. Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams. BMC Vet Res 9:51. DOI: https://doi.org/10.1186/1746-6148-9-51
Santos FA, da Costa DF, da Silva AF, Dos Santos Pessoa RM, Cambuí Figueiredo Rocha V, Gomes Olinda R, Dantas AFM, Almeida de Melo M, Peña Alfaro CE, Azevedo SS, Alves CJ, 2019. Microbiological, molecular, and histopathological findings in goats experimentally infected with Actinobacillus seminis. Microb Pathog 133:103555. DOI: https://doi.org/10.1016/j.micpath.2019.103555
Seemann T, 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068-9. DOI: https://doi.org/10.1093/bioinformatics/btu153
Stothard P, Wishart DS, 2005. Circular genome visualization and exploration using CGView. Bioinformatics 21:537-9. DOI: https://doi.org/10.1093/bioinformatics/bti054
Thompson JE, 2022. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in veterinary medicine. Vet World 15:2623-57. DOI: https://doi.org/10.14202/vetworld.2022.2623-2657
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