Retrospective analysis of Vibrio spp. isolated from marketed crustaceans using multilocus sequence analysis
https://doi.org/10.4081/ijfs.2023.11045
Accepted: 1 February 2023
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The genus Vibrio includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (MLSA) scheme developed on the basis of 4 housekeeping genes (gyrB, pyrH, recA and atpA) was applied to identify 92 Vibrio strains isolated from crustaceans in 2011. Concatenated sequences were used for the phylogenetic and population analyses and the results were compared with those from biochemical identification tests. From the phylogenetic analysis, 10 clusters and 4 singletons emerged, whereas the population analysis highlighted 12 subpopulations that were well supported by phylogeny with few exceptions. The retrospective analysis allowed correct re-attribution of isolated species, indicating how, for some pathogens, there may be an overestimation of phenotypic identification (e.g. V. parahaemolyticus). Use of the PubMLST Vibrio database highlighted a possible genetic link between Sequence Type (ST) 529 and ST195 (V. alginolyticus) isolated from a human case in Norway during 2018. In addition to the identification of major risk groups of V. cholerae, V. vulnificus and V. parahaemolyticus, MLSA could be a valid support for species considered a minor risk, such as V. alginolyticus, V. mimicus and V. fluvialis. Due to the increased incidence of vibriosis in Europe, the application of different tools will also have to be considered to investigate the possible epidemiological links of the various species in the perspective of Open Science to protect the consumer.
Álvarez-Contreras AK, Quiñones-Ramírez EI, Vázquez-Salinas C, 2021. Prevalence, detection of virulence genes and antimicrobial susceptibility of pathogen Vibrio species isolated from different types of seafood samples at “La Nueva Viga” market in Mexico City. Antonie van Leeuwenhoek 114:1417–29. DOI: https://doi.org/10.1007/s10482-021-01591-x
Amato E, Riess M, Thomas-lopez D, Linkevicius M, Pitkänen T, Wołkowicz T, Rjabinina J , Jernberg C, Hjertqvist M, macdonald E, Antony-Samy JK, Bjerre KD, Saara Salmenlinna S, Fuursted K, Hansen A, Naseer U, 2022. Epidemiological and microbiological investigation of a large increase in vibriosis, northern Europe, 2018. Euro Surveill 27:2101088. DOI: https://doi.org/10.2807/1560-7917.ES.2022.27.28.2101088
Austin B, 2010. Vibrios as causal agents of zoonoses. Vet Microbiol 140:310–7. DOI: https://doi.org/10.1016/j.vetmic.2009.03.015
Bej AK, Patterson DP, Brasher CW, Vickery MCL, Jones DD, Kaysner CA, 1999. Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh. J Microbiol Methods 36:215–25. DOI: https://doi.org/10.1016/S0167-7012(99)00037-8
Brehm TT, Berneking L, Martins MS, Dupke S, Jacob D, Drechsel O, Bohnert J, Becker K, Kramer A, Christner M, Aepfelbacher M, Schmiedel S, Rohde H, Balau V, Baufeld E, Brechmann S, Briedigkeit L, Diedrich S, Ebert U, Fickenscher H, Grgic B, Heidecke CD, Hinz P, Hoffmann A, Holbe M, Ignatius R, Kaup O, Kern M, Kerwat M, Klempien I, Lamprecht G, Meerbach A, Mischnik A, Podbielski A, Schaefer S, Schwarz R, Strauch E, Warnke P, Weikert-Asbec, S, Witte M, Zaki W, 2021. Heatwave-associated Vibrio infections in Germany, 2018 and 2019. Euro Surveill 26:2002041. DOI: https://doi.org/10.2807/1560-7917.ES.2021.26.41.2002041
Caburlotto G, Suffredini E, Toson M, Fasolato L, Antonetti P, Zambon M, Manfrin A, 2016. Occurrence and molecular characterisation of Vibrio parahaemolyticus in crustaceans commercialised in Venice area, Italy. Int J Food Microbiol 220:39–49. DOI: https://doi.org/10.1016/j.ijfoodmicro.2015.12.007
Conferenza stato regioni, Intesa Stato Regioni 212/2016, 2016. 854. 882 212/16. Linee guida per il controllo ufficiale ai sensi dei Regolamenti (CE) 882-854.
ECDC E.C. for D.P. and C., 2021. Communicable disease threats.
European Commission, 2004. Regulation of European parliament and of the Council of 29 April 2004 (EC) on official controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules 882/2004/CE. In: Official Journal. L165/1. 30/4/2004 No longer in force, Date of end of validity: 13/12/2019.
European Commission, 2004. Regulation of European parliament and of the Council of 29 April 2004 (EC) laying down specific rules for the organisation of official controls on products of animal origin intended for human consumption 854/2004 /CE. In: Official Journal. L155/206. 30/4/2004 No longer in force, Date of end of validity: 13/12/2019.
European Commission, 2017. Regulation of European parliament and of the Council of 15 March 2017 (EC) on official controls and other official activities performed to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant protection products, amending Regulations (EC) No 999/2001, (EC) No 396/2005, (EC) No 1069/2009, (EC) No 1107/2009, (EU) No 1151/2012, (EU) No 652/2014, (EU) 2016/429 and (EU) 2016/2031 of the European Parliament and of the Council, Council Regulations (EC) No 1/2005 and (EC) No 1099/2009 and Council Directives 98/58/EC, 1999/74/EC, 2007/43/EC, 2008/119/EC and 2008/120/EC, and repealing Regulations (EC) No 854/2004 and (EC) No 882/2004 of the European Parliament and of the Council, Council Directives 89/608/EEC, 89/662/EEC, 90/425/EEC, 91/496/EEC, 96/23/EC, 96/93/EC and 97/78/EC and Council Decision 92/438/EEC (Official Controls Regulation) 625/2017/CE. In: Official Journal. L95/1. 7/4/2017.
Falush D, Stephens M, Pritchard JK, 2003. Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567–87. DOI: https://doi.org/10.1093/genetics/164.4.1567
Harrison J, Nelson K, Morcrette H, Morcrette C, Preston J, Helmer L, Titball RW, Butler CS, Wagley S, 2022. The increased prevalence of Vibrio species and the first reporting of Vibrio jasicida and Vibrio rotiferianus at UK shellfish sites. Water Res 211:117942. DOI: https://doi.org/10.1016/j.watres.2021.117942
Hartnell RE, Stockley L, Keay W, Rosec JP, Hervio-Heath D, Van den Berg H, Leoni F, Ottaviani D, Henigman U, Denayer S, Serbruyns B, Georgsson F, Krumova-Valcheva G, Gyurova E, Blanco C, Copin S, Strauch E, Wieczorek K, Lopatek M, Britova A, Hardouin G, Lombard B, in’t Veld P, Leclercq A, Baker-Austin C, 2019. A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods. Int J Food Microbiol 288:58–65. DOI: https://doi.org/10.1016/j.ijfoodmicro.2018.02.008
ISO, 2017. Microbiology of the food chain — Horizontal method for the determination of Vibrio spp. — Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. Norm 21872-1 International Standardization Organization ed., Geneva, Switzerland.
Kim YB, Okuda J, Matsumoto C, Takahashi N, Hashimoto S, Nishibuchi M, 1999. Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J Clin Microbiol 37:1173–7. DOI: https://doi.org/10.1128/JCM.37.4.1173-1177.1999
Koralage MSG, Alter T, Pichpol D, Strauch E, Zessin K-H, Huehn S, 2012. Prevalence and Molecular Characteristics of Vibrio spp. Isolated from Preharvest Shrimp of the North Western Province of Sri Lanka. J Food Prot 75:1846–50. DOI: https://doi.org/10.4315/0362-028X.JFP-12-115
Neill MA, 2020. Other Pathogenic Vibrios. In Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Philadelphia (PA): Saunders. Pp.2645-9.
Onohuean H, Agwu E, Nwodo UU, 2022. A Global Perspective of Vibrio species and associated diseases: three-decade meta-synthesis of research advancement. Environ Health Insights 16:11786302221099406. DOI: https://doi.org/10.1177/11786302221099406
Pretto T, 2020. Vibriosis due to Vibrio harveyi: etiopathogenesis and vaccine efficacy studies in sea bass (Dicentrarchus labrax). Degree Diss., Alma Mater Stud. Università di Bologna, Italy.
Rahman MS, Carraro R, Cardazzo B, Carraro L, Boscolo Meneguolo D, Martino ME, Andreani NA, Bordin P, Mioni R, Barco L, Novelli E, Fasolato L, 2017. Molecular typing of Vibrio parahaemolyticus strains isolated from mollusks in the North Adriatic Sea. Foodborne Pathog Dis 14:454-64. DOI: https://doi.org/10.1089/fpd.2016.2263
Rahman MS, Martino ME, Cardazzo B, Facco P, Bordin P, Mioni R, Novelli E, Fasolato L, 2014. Vibrio trends in the ecology of the Venice lagoon. Appl Environ Microbiol 80:2372-80. DOI: https://doi.org/10.1128/AEM.04133-13
Sampaio A, Silva V, Poeta P, Aonofriesei F, 2022. Vibrio spp.: Life strategies, ecology, and risks in a changing environment. Diversity 14:1–26. DOI: https://doi.org/10.3390/d14020097
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731-9. DOI: https://doi.org/10.1093/molbev/msr121
Traoré SG, Bonfoh B, Krabi R, Odermatt P, Utzinger J, Rose KN, Tanner M, Frey J, Quilici ML, Koussémon M, 2012. Risk of Vibrio transmission linked to the consumption of crustaceans in coastal towns of Côte d'Ivoire. J Food Prot 75:1004-11. DOI: https://doi.org/10.4315/0362-028X.JFP-11-472
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