MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY

  • A. Petruzzelli | lucia.zoppi@pagepress.org Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia, Italy.
  • F. Ciarrocchi Dipartimento di Scienze Biomolecolari, Università degli studi di Urbino “Carlo Bo” – Urbino, Italy.
  • M. Foglini Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia, Italy.
  • S. Fisichella Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia, Italy.
  • C. Sebastiani Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia, Italy.
  • W. Baffone Dipartimento di Scienze Biomolecolari, Università degli studi di Urbino “Carlo Bo” – Urbino., Italy.
  • S. Santarelli Dipartimento SAIFET, Università Politecnica delle Marche – Ancona, Italy.
  • F. Tonucci Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia., Italy.
  • G. Blasi Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia., Italy.

Abstract

The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and culturebased methods. The estimation of genotypic intra-species variation of the autochthonous bacteria isolated was also evaluated by using randomly amplified polymorphic DNA (RAPD) analysis and unweighted pairgroup method with arithmetic averages (UPGMA) cluster analysis. Our findings revealed an high diversity of the autochthonous bacterial population investigated, both at species and strain level.

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Published
2011-01-08
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Short Communications
Keywords:
Ciauscolo, autochthonous bacteria, RAPD.
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How to Cite
Petruzzelli, A., Ciarrocchi, F., Foglini, M., Fisichella, S., Sebastiani, C., Baffone, W., Santarelli, S., Tonucci, F., & Blasi, G. (2011). MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY. Italian Journal of Food Safety, 1(1zero), 287-288. https://doi.org/10.4081/ijfs.2011.892