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Characterization of non-typhoidal Salmonella enterica strains of human origin in central and southern Italy

Yolande T.R. Proroga, Federico Capuano, Rosanna Capparelli, Stefano Bilei, Mariano Bernardo, Maria Pia Cocco, Rosalba Campagnuolo, Vincenzo Pasquale
  • Yolande T.R. Proroga
    Department of Food Microbiology, Regional Laboratory for Salmonella Serotyping, Institute for Experimental Veterinary Medicine of Southern Italy, Portici, Italy
  • Federico Capuano
    Department of Food Microbiology, Regional Laboratory for Salmonella Serotyping, Institute for Experimental Veterinary Medicine of Southern Italy, Portici, Italy | federico.capuano@cert.izsmportici.it
  • Rosanna Capparelli
    Department of Agriculture, University of Naples Federico II, Portici, Italy
  • Stefano Bilei
    Department of Food Microbiology, Institute for Experimental Veterinary Medicine of the Lazio and Tuscany Regions M. Aleandri, Rome, Italy
  • Mariano Bernardo
    Microbiology and Virology Laboratory, Specialist Hospital Monaldi-Cotugno-CTO, Naples, Italy
  • Maria Pia Cocco
    Laboratory of Clinical Pathology, Local Health Unit Naples 2 North, Pozzuoli, Italy
  • Rosalba Campagnuolo
    Laboratory of Clinical Pathology and Microbiology, A.O.R.N. Santobono-Pausilipon, Naples, Italy
  • Vincenzo Pasquale
    Department of Science and Technology, University of Naples Parthenope, Naples, Italy

Abstract

Non-typhoidal Salmonella enterica infection is a significant public health problem worldwide. The aim of this study was to characterize Salmonella enterica strains isolated from human specimens in central and southern Italy, for epidemiological studies. One hundred and fifty S. enterica strains were serotyped. Isolates were tested for their antimicrobial susceptibility, by disk diffusion method. The molecular characterizations, based on PCR, were carried out for the detection of invA gene and other virulence elements and phage marker genes. Eighteen different Salmonella serotypes were identified. The most common serotypes detected were S. Typhimurium, S. Enteritidis, the monophasic variant of S. Typhimurium (S. 4,[5],12:i:-), and S. Napoli. High resistance rates were recorded for tetracycline (64%), streptomycin (62%), sulphonamide (57%), and ampicillin (56%). The ASSuT R-type, also associated to resistance to other antibiotics, was highly prevalent in S. 4,[5],12:i:- (97%) and S. Typhimurium (55%), while the ACSSuT Rtype, also associated to other antibiotics, was observed prevalently in S. Typhimurium (20.4%). The genes of more common detection were invA (100%), sspH2 (86.6%), gtgB (84.6%), g8 (80%), sodC1 (77.3%), gipA (52.6%), sspH1 (52.6%).

Keywords

Salmonella enterica, human isolates, antibiotic resistance, R-type ASSuT, Rtype ACSSuT, virulotype

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Submitted: 2017-07-07 15:32:24
Published: 2018-03-31 00:00:00
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