Evaluation of Real-Time PCR to complement ISO 6579:2004 method for the detection of Salmonella in pork cuts

Submitted: 4 January 2013
Accepted: 4 January 2013
Published: 31 October 2012
Abstract Views: 3173
PDF: 1964
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

According to Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological crite-ria for foodstuff , the analytical reference method for the detection of Salmonella in food is ISO 6579:2004. However this long and labor-intensive method is not in line with the short production times of the food industry. In the last years, Real-Time PCR is used more and more by scientists for the relia-ble, fast and specific detection of bacterial pathogens in food. The aim of the present study was to eval-uate the Salmonella detection capability of a validated Real-Time PCR assay on naturally contaminated pork cuts in comparison with the reference method ISO 6579:2004. Three sampling were performed and included 16 pork cut packaging. From each packaging, three aliquots of 10 g each were tested separate-ly by ISO 6579:2004 method and by Real-Time PCR. In particular this molecular method was applied on DNA samples extracted from pre-enrichment broth after 1 and 18 hours of incubation. Within the three sampling periods, Real-Time PCR detected Salmonella in 81%, 100% e 62,5% of pork cut samples respectively, whereas the corresponding percentages of detection of the reference method were 56%, 81% e 62,5% respectively. In conclusion the Real-Time PCR assay used in the present study might be a reliable tool for a fast detection of Salmonella on pork cuts, especially when large number of samples needs to be tested. The reference method might be applied only on positive samples for isolation purpos-es mandatory in epidemiological investigations.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

How to Cite

1.
Pasquali F, Bovo F, Alex L, Alessandra DC, Gerardo M. Evaluation of Real-Time PCR to complement ISO 6579:2004 method for the detection of Salmonella in pork cuts. Ital J Food Safety [Internet]. 2012 Oct. 31 [cited 2024 Dec. 3];1(5):11-5. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2012.5.11