Thymus vulgaris L. as a possible effective substitute for nitrates in meat products

Submitted: 3 August 2018
Accepted: 8 October 2019
Published: 19 August 2020
Abstract Views: 738
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Nitrates are chemicals found naturally in some foods such as fruit and vegetables or added to others, especially meats, as a preservative. Their use as additives is regulated by European Commission to avoid any risk for human health. In order to reduce or replace the use of these compounds, we investigated the bacteriostatic/bactericidal activity of the essential oil of Thymus vulgaris L. against Listeria innocua, a nonpathogenic microorganism with the same morpho-cultural traits of L. monocytogenes. The study was carried out in vitro and in vivo on processed meat products, i.e. mature salami, by using thyme essential oil. Although the results are preliminary, the antilisterial activity of the thyme essential oil was shown to be similar to that of nitrates.



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Al-Asmari A, Athar M, Al-Faraidy A, Almuhaiza M, 2017. Chemical composition of essential oil of Thymus vulgaris collected from Saudi Arabian market. Asian Pac J Trop Biomed 7:147-50. DOI:
Barfroshan Y, Mottaghipisheh J, Maghsoodlou M-T, Dosti N, Beyzaei H, Moghaddam Manesh MR, Vitalini S, Iriti M, 2018. Comparative Study of Bioactivities and Chemical Constituents of Cymbopogon jwarancusa subsp. olivieri (Boiss.) Soenarko Harvested in Spring and Winter. Journal of Essential Oil Bearing Plants 21(4):1107-8. DOI:
Burt S, 2004. Essential oils: their antibacterial properties and potential applications in food – a review. Int J Food Microbiol 94:223-53. DOI:
Boveiri Dehsheikh A, Mahmoodi Sourestani M, Boveiri Dehsheikh P, Vitalini S, Iriti M, Mottaghipisheh J, 2019. A Comparative Study of Essential Oil Constituents and Phenolic Compounds of Arabian Lilac (Vitex Trifolia var. Purpurea): An Evidence of Season Effects. Foods 8(2):52. DOI:
Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W, 2016. Essential oils’ chemical characterization and investigation of some biological activities: a critical review. Medicines 3:25. DOI:
Dragoni I, Vallone L, 2009. Attività antifungina dell’olio essenziale di rosmarino nei confronti di specie di interesse alimentare. Rivista dell’Associazione Italiana Veterinari Igienisti 4:17-20.
EFSA 2017.
El-Alam I, Zgheib R, Iriti M, El Beyrouthy M, Hattouny P, Verdin A, Fontaine J, Chahine R, Lounès-Hadj Sahraoui A, Makhlouf H, 2019. Origanum syriacum Essential Oil Chemical Polymorphism According to Soil Type. Foods 8(3):90. DOI:
Fachini-Queiroz FC, Kummer R, Estevao-Silva CF, Carvalho MD, Cunha JM, 2012. Effects of thymol and carvacrol, constituents of Thymus vulgaris L. essential oil, on the inflammatory response. Evid Based Complement Alternat Med 2012:1-10. DOI:
Fernández-López J, Viuda-Martos M, 2018. Introduction to the special issue: application of essential oils in food systems. Foods 7:56. DOI:
FDA, 2017.
Fuenmayora CA, Mascheronia E, Cosio MS, Piergiovanni L, Benedetti S, Ortenzi M, Schiraldi A, Mannino S, 2013. Encapsulation of R-(+)-limonene in edible electrospun nanofibers. Chem Eng Trans 32:1771-6.
García-García R, López-Malo A, Palou E, 2011. Bactericidal action of binary and ternary mixtures of carvacrol, thymol, and eugenol against Listeria innocua. J Food Sci 76: M95-M100. DOI:
Giarratana F, Muscolino D, Ragonese C, Beninati C, Sciarrone D, Ziino G, Mondello L, Giuffrida A, Panebianco A, 2016. Antimicrobial activity of combined thyme and rosemary essential oils against Listeria monocytogenes in Italian mortadella packaged modified atmosphere. J Essent Oil Res 28:467-74. DOI:
Hyldgaard M, Mygind T, Meyer RL, 2012. Essential oils in food preservation: mode of action, synergies and interactions with food matrix components. Front Microbiol 3:12. DOI:
Jarić S, Mitrović M, Pavlović P, 2015. Review of ethnobotanical, phytochemical, and pharmacological study of Thymus serpyllum L. Evid Based Complement Alternat Med 2015: 1-10. DOI:
Koo OK, Eggleton M, O'Bryan CA, Crandall PG, Ricke SC, 2012. Antimicrobial activity of lactic acid bacteria against Listeria monocytogenes on frankfurters formulated with and without lactate/diacetate. Meat Sci 92:533-7. DOI:
Meloni D, 2015. Presence of Listeria monocytogenes in Mediterranean-style dry fermented sausages. Foods 4(1):34. DOI:
Paparella A, Serio A, Chaves Lòpez C, Mazzarrino G, 2013. Plant-based intervention strategies for Listeria monocytogenes control in foods. In A. Mendez-Vilas (Ed.), Microbial pathogens and strategies for combating them: Science, technology and education. Formatex Research Center, Zurbaran, 06002 Badajoz, Spain. PP: 1230-46.
Vitalini S, Madeo M, Tava A, Iriti M, Vallone L, Avato P, Cocuzza C, Simonetti P, Argentieri M, 2016. Chemical profile, antioxidant and antibacterial activities of Achillea moschata wulfen, an endemic species from the alps. Molecules 21(7):830. DOI:
Viuda-martos M, Ruiz-Navajas Y, Fernàndez-Lòpez J, Pèeèz-Alvarez JA, 2010. Effect of added citrus fibre and spice essential oils on quality characteritics and shelf-life of mortadella. Meat Sci 85:568-76. DOI:

How to Cite

Salvaneschi S, Iriti M, Vitalini S, Vallone L. <em>Thymus vulgaris</em> L. as a possible effective substitute for nitrates in meat products. Ital J Food Safety [Internet]. 2020 Aug. 19 [cited 2024 Jul. 15];9(2). Available from:

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