Dissemination and persistence of Pseudomonas spp. in small-scale dairy farms


Submitted: 29 November 2015
Accepted: 8 February 2016
Published: 2 May 2016
Abstract Views: 1627
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This study was aimed at collecting data on presence, dissemination and persistence of Pseudomonas in small-scale dairy farms. Six farms (located in Piedmont) were visited three times over 2014: 116 waters (wells and different faucets/pipes) and 117 environmental samples (milking equipments and drains) were collected. Enumeration of Pseudomonadaceae was performed, 3-5 colonies/samples were selected for identification via 16SrDNA/oprI polymerase chain reaction (PCR), and typed by enterobacterial-repetitive- intergenic-consensus (ERIC)-PCR. Pseudomonadaceae were detected in 77% of samples. No statistical differences were found among proportions of positives across farms, sample typologies and seasons. Most isolates were Pseudomonas fluorescens (45%), and ERIC-PCR showed 32 persistent types diffused across farms. All in all, Pseudomonas spp. represents a challenge, considering its presence over time in water as well as in teat cups, indicating a continuous source of contamination. Moreover, persistency of strains may indicate biofilm-formation and/or sanitisers resistance, therefore emphasising the role of primary production for preventing milk contamination by Pseudomonas spp.

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Regione Piemonte

1.
Nucera DM, Lomonaco S, Morra P, Ortoffi MF, Giaccone D, Grassi MA. Dissemination and persistence of Pseudomonas spp. in small-scale dairy farms. Ital J Food Safety [Internet]. 2016 May 2 [cited 2024 Jun. 13];5(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/5652

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