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Probiotic-fortified Solanum lycopersicum (tomato) juice: free or encapsulated Lactobacillus plantarum and Lactobacillus delbrueckii

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Received: 28 April 2025
Published: 20 October 2025
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Recent consumer perception of a nutritious diet improves demand for functional and safety products such as probiotics. The present research aims to investigate enriching Solanum lycopersicum (tomato) juice, including free and encapsulated probiotic bacteria. Initially, physicochemical attributes of encapsulations were evaluated. Then tomato juice samples, including a control without bacteria, free or encapsulated Lactobacillus plantarum (L. plantarum, T1 and T2), Lactobacillus delbrueckii (L. delbrueckii, T3 and T4), and a mixture (T5 and T6), were prepared over 28 days of shelf life. Several assays were performed, such as pH, lycopene, turbidity, stability, antioxidant, probiotic viability, sensory, and structure. Physicochemical functions of encapsulation illustrated that the results were in the suitable range. The pH of all treatments declined, and free L. plantarum demonstrated a greater effect on reduction. The control and encapsulated L. plantarum samples exhibited the lowest lycopene, ranging from 0.64 to 0.35 μL/mL, while the highest ranged from 0.64 to 0.50 μL/mL during the shelf life. Encapsulated dual bacteria indicated higher turbidity, stability, and antioxidant features compared to the control throughout shelf life. The control maintained greater transparency than others, and microbial analysis indicated that probiotic populations were elevated until the 14th day and then reduced. The encapsulated dual-bacteria illustrated the maximum viability and sensory, while the control had the minimum ratings. Morphological analysis confirmed a homogeneous structure for encapsulated bacteria. Overall results depicted that treatments containing encapsulated bacteria are considered the preferred option to promote nutritious juice.

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Ahmadmoradi M, Alishahi M, Soltanian S, Shahriari A, Yektaseresht A, 2024. Effects of encapsulation of Lactobacillus plantarum on probiotic potential and reducing lead toxicity in rainbow trout (Oncorhynchus mykiss Walbaum). Aquac Int 32:337-59.

Annan NT, Borza AD, Hansen LT, 2008. Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal conditions. Int Food Res 41:184-93.

Budiati T, Suryaningsih W, Bethiana TN, 2022. Antimicrobial of tropical fruit and vegetable waste extract for food-borne pathogenic bacteria. Ital J Food Safety 11:10510.

Castello A, Cascio GL, Ferraro C, Pantano L, Costa A, Butera G, Oliveri G, Rizzuto ML, Alduina R, Cardamone C, 2023. Food risk associated with vegetable consumption, exposure to antimicrobial-resistant strains and pesticide residues. Ital J Food Safety 12:11134.

Chung HJ, Lee H, Na G, Jung H, Kim DG, Shin SI, Jung SE, Choi ID, Lee JH, Sim JH, Choi HK, 2020. Metabolic and lipidomic profiling of vegetable juices fermented with various probiotics. Biomolecules 10:725-35.

Da Silva SÂ, Batista LD, Diniz DS, Nascimento SS, Morais NS, de Assis CF, Passos TS, De Sousa Júnior FC, 2023. Microencapsulation of probiotics by oil-in-water emulsification technique improves cell viability under different storage conditions. Foods 12:252-64.

Dzandu B, Chotiko A, Sathivel S, 2022. Antioxidant activity and viability of Lacticaseibacillus rhamnosus, Lacticaseibacillus casei, and co-culture in fermented tomato juice during refrigerated storage. Food Biosci 50:102085.

Ephrem E, Najjar A, Charcosset C, Greige-Gerges H, 2018. Encapsulation of natural active compounds, enzymes, and probiotics for fruit juice fortification, preservation, and processing: an overview. J Funct Foods 48:65-84.

Fernandes N, Achemchem F, Gonzales-Barron U, Cadavez V, 2024. Biopreservation strategies using bacteriocins to control meat spoilage and foodborne outbreaks. Ital J Food Safety 13:12558.

Gan C, Langa E, Valenzuela A, Ballestero D, Pino-Otín MR, 2023. Synergistic activity of thymol with commercial antibiotics against critical and high WHO priority pathogenic bacteria. Plants 12:1868-78.

Ghafari S, Ansari S, 2018. Microbial viability, physico-chemical properties and sensory evaluation of pineapple juice enriched with Lactobacillus casei, Lactobacillus rhamnosus and inulin during refrigerated storage. J Food Meas Charact 12:1-9.

Ghobadi Dana M, Rashnavadi T, 2016. Investigation on the influence of two layer microencapsulation by calcium alginate on survivability of Lactobacillus acidophilus in tomato juice during shelf life. Innov Food Technol 4:151-60.

Giordano I, Abuqwider J, Altamimi M, Di Monaco R, Puleo S, Mauriello G, 2022. Application of ultrasound and microencapsulation on Limosilactobacillus reuteri DSM 17938 as a metabolic attenuation strategy for tomato juice probiotication. Heliyon 8:10969.

Goderska K, Dombhare K, Radziejewska-Kubzdela E, 2022. Evaluation of probiotics in vegetable juices: tomato (Solanum lycopersicum), carrot (Daucus carota subsp. sativus) and beetroot juice (Beta vulgaris). Arch Microbiol 204:300-15.

Isnaini AA, Trimulyono G, 2024. Aktivitas antibakteri coconut yoghurt terhadap Streptococcus mutans secara in Vitro. LenteraBio: Berkala Ilmiah Biologi 13:393-401. [Article in Indonesian].

Kaur S, Kaur HP, Grover J, 2016. Fermentation of tomato juice by probiotic lactic acid bacteria. Int J Adv Pharm Biol Chem 5:212-9.

Liu Y, Chen H, Chen W, Zhong Q, Zhang G, Chen W, 2018. Beneficial effects of tomato juice fermented by Lactobacillus plantarum and Lactobacillus casei: antioxidation, antimicrobial effect, and volatile profiles. Molecules 23:2366-79.

Ma F, Ma R, Zhao L, 2025. Effects of antimicrobial peptides on antioxidant properties, non-specific immune response and gut microbes of tsinling lenok trout (Brachymystax lenok tsinlingensis). Biochem Genet 63:85-103.

Mantzourani I, Terpou A, Alexopoulos A, Bezirtzoglou E, Bekatorou A, Plessas S, 2019. Production of a potentially synbiotic fermented Cornelian cherry (Cornus mas L.) beverage using Lactobacillus paracasei K5 immobilized on wheat bran. Biocatal Agric Biotechnol 17:347-51.

Martin MJ, Lara-Villoslada F, Ruiz MA, Morales ME, 2013. Effect of unmodified starch on viability of alginate-encapsulated Lactobacillus fermentum CECT5716. LWT 53:480-6.

Naga SS, Ramesh B, Umamahesh K, Vijaya SRO, 2016. Probiotication of tomato and carrot juices for shelf-life enhancement using micro-encapsulation. J Food Biosci Technol 6:13-22.

Nikolaou A, Mitropoulou G, Nelios G, Kourkoutas Y, 2023. Novel functional grape juices fortified with free or immobilized Lacticaseibacillus rhamnosus OLXAL-1. Microorganisms 11:646-58.

Othman NB, Roblain D, Chammen N, Thonart P, Hamdi M, 2009. Antioxidant phenolic compounds loss during the fermentation of Chétoui olives. Food Chem 116:662-9.

Özdemir N, 2022. A multi-functional survey of the properties of Lacticaseibacillus paracasei subsp. tolerans NOC-122, Levilactobacillus parabrevis NOC-111 and Latilactobacillus curvatus NOC-110. Microbiology 168:001239.

Pereira N, Farrokhi M, Vida M, Lageiro M, Ramos AC, Vieira MC, Alegria C, Gonçalves EM, Abreu M, 2023. Valorisation of wasted immature tomato to innovative fermented functional foods. Foods 12:1532-45.

Rengadu D, Gerrano AS, Mellem JJ, 2021. Microencapsulation of Lactobacillus casei and Bifidobacterium animalis enriched with resistant starch from vigna unguiculata. Starch‐Stärke 73:2000247.

Wang W, Xu H, Ye Q, Tao F, Wheeldon I, Yuan A, Hu Y, Wu J, 2022. Systemic immune responses to irradiated tumours via the transport of antigens to the tumour periphery by injected flagellate bacteria. Nat Biomed Eng 6:44-53.

Yang F, Wang YP, Zhao H, 2019. Quality enhancement of fermented vegetable juice by probiotic through fermented yam juice using Saccharomyces cerevisiae. Food Sci Technol 40:26-35.

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1.
Probiotic-fortified Solanum lycopersicum (tomato) juice: free or encapsulated Lactobacillus plantarum and Lactobacillus delbrueckii. Ital J Food Safety [Internet]. 2025 Oct. 20 [cited 2026 Apr. 29];14(4). Available from: https://www.pagepressjournals.org/ijfs/article/view/13932