Effect of production process and high-pressure processing on viability of Salmonella spp. in traditional Italian dry-cured coppa


Submitted: 25 July 2019
Accepted: 8 January 2020
Published: 19 August 2020
Abstract Views: 881
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Authors

  • Roberta Taddei Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.
  • Federica Giacometti Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano dell’Emilia (BO), Italy.
  • Lia Bardasi Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.
  • Paolo Bonilauri Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Reggio Emilia, Reggio nell’Emilia, Italy.
  • Mattia Ramini Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.
  • Maria Cristina Fonatana Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.
  • Patrizia Bassi Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.
  • Sara Castagnini Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.
  • Francesco Ceredi Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.
  • Maria Francesca Pelliconi Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.
  • Andrea Serraino Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano dell’Emilia (BO), Italy.
  • Federico Tomasello Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano dell’Emilia (BO), Italy.
  • Silvia Piva Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano dell’Emilia (BO), Italy.
  • Elisabetta Mondo Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano dell’Emilia (BO), Italy.
  • Giuseppe Merialdi Istituto Zooprofilattico Sperimentale delle Lombardia e dell’Emilia Romagna, Sede Territoriale di Bologna, Italy.

The aim of the study was to investigate the combined effect of the manufacturing process followed by HPP treatment on the inactivation of Salmonella spp. in artificially contaminated coppa samples, in order to verify the ability of the combined processes to achieve the objective of a 5-log reduction of Salmonella spp. needed for exportation to the U.S. Fresh anatomical cuts intended for coppa production were supplied by four different delicatessen factories located in Northern Italy. Raw meat underwent experimental contamination with Salmonella spp. using a mixture of 3 strains. Surface contamination of the fresh anatomical cuts was carried out by immersion into inoculum containing Salmonella spp. The conditions of the HPP treatment were: pressure 593 MPa, time 290 seconds, water treatment temperature 14°C. Surface and deep samples were performed post contamination (T0), end of the cold phase (T1), end of process (Tend), and after HPP treatment (postHPP) and Salmonella spp. Enumerated. The results of this study show a significant reduction of Salmonella spp. all through the production process (P<0.01) for all companies, followed by an additional reduction of bacterial counts due to HPP treatment (P<0.01), both in superficial and deep contaminations (P<0.01). The superficial overall reduction resulted of 1.58 to 5.04 log CFU/g during the production process. HPP treatment resulted in a significant (P<0.01) superficial and deep decrease in Salmonella spp. enumeration varying from 0.61 to 4.01 log and from 1.49 to 4.13 log. According to the data presented in this study, only the combined approach of coppa manufacturing process followed by HPP treatment always led to a 5-log reduction of Salmonella spp. required by USDA/FSIS guidelines.


Anthoula AA, Papadopoulou OS, Nisiotou A, Tassou CC, Chorianopoulos N, 2018. Effect of high-pressure processing on the survival of Salmonella enteritidis and shelf-life of chicken fillets. Food Microbiol 70, 55-64.

Black EP, Huppertz THM, Kelly AL, Fitzgerald GF, 2007. Baroprotection of vegetative bacteria by milk constituents: a study of Listeria innocua. Int Dairy J 17:104-110.

Black EP, Setlow P, Hocking AD, Stewart CM, Kelly AL, Hoover DG, 2007. Response of spores to high-pressure processing. Compr Rev Food Sci and Food Saf 6:103-119.

Bonilauri p, Grisenti MS, Daminelli P, Merialdi G, Ramini M, Bardasi L, Taddei R, Cosciani-Cunico E, Dalzini E, Frustoli MA, Giacometti F, Piva S, Serraino A. 2019. Reduction of Salmonella spp. populations in Italian salami during production process and High Pressure Processing treatment: validation of processes to export to the U.S. Meat Science 157: 107869.

Bover-Cid S, Belletti N, Aymerich T, Garriga M, 2017. Modelling the impact of water activity and fat content of dry-cured ham on the reduction of Salmonella enterica by high pressure processing. Meat Sci. 123:120-125.

Busconi M, Zacconi C, Scolari G. 2014. Bacterial ecology of PDO Coppa and Pancetta Piacentina at the end of ripening and after MAP storage of sliced product. Int. J. of Food Microbiol 172, 13-20

European Community. 2005. Commission Regulation (EC) No 2073/2005 of 15 November 2005 on microbiological criteria for foodstuffs.

European Food Safety Authority. 2010. Management of left-censored data in dietary exposure assessment of chemical substances. EFSA Journal. 8(3):1557.

FSIS, 2017. Salmonella Compliance Guidelines for Small and Very Small Meat and Poultry Establishments that Produce Ready-to-Eat (RTE) Products and Revised Appendix A June 2017.

Garriga M, Grebol N, Aymerich MT, Monfort JM, Hugas M, 2004. Microbial inactivation after high-pressure processing at 600 MPa in commercial meat products over its shelf life. nnov Food Sci Emerg Technol 5:451–457.

Garriga M, Marcos B, Aymerich T, Hugas M, 2003. Prospectiva de aplicación de altas presiones para la minimización de riesgos asociados a Salmonella y Listeria monocytogenes en embutidos madurados en frío. Eurocarne 121:6.

Hayman MM, Baxter I, O’Riordan PJ, Stewert CM, 2004. Effects of High-Pressure Processing on the Safety, Quality, and Shelf Life of Ready-to-Eat Meats. JFP 67(8):1709-18.

Hayman MM, Kouassi GK, Anantheswaran RC, Floros JD, Knabel SJ, 2008. Effect of water activity on inactivation of Listeria monocytogenes and lactate dehydrogenase during high pressure processing. Int J Food Microbiol 124:21-26.

Hugas M, Garriga M, Monfort JM, 2002. New mild technologies in meat processing: high pressure as a model technology. Meat Sci. 62:359-371.

ISO 21807:2004. Microbiology of food and animal feeding stuffs — Determination of water activity.

ISO 6579:2002/Cor1:2004. Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp.

Italian Ministry of Health, 2015. Export verso gli USA di prodotti a base di carne: rimosse le misure restrittive.

Lerasle M, Guillou S, Simonin H, Anthoine V, Chéret R, Federighi M, Membré JM, 2014. Assessment of Salmonella and Listeria monocytogenes level in ready-to-cook poultry meat: effect of various high-pressure treatments and potassium lactate concentrations. Int J Food Microbiol 186, 74-83.

Morales-Partera ÁM, Cardoso-Toset F, Jurado-Martos F, Astorga RJ, Huerta B, Luque I, Tarradas C, Gómez-Laguna J, 2017. Survival of selected foodborne pathogens on dry cured pork loins. Int J Food Microbiol 258:68-72.

Patterson MF, 2005. Microbiology of pressure-treated foods. J Appl Microbiol 98:1400-1409.

Pin C, Avendaño-Perez G, Cosciani-Cunico E, Gómez N, Gounadakic A, Nychas GJ, Skandamis P, Barker G, 2011. Modelling Salmonella concentration throughout the pork supply chain by considering growth and survival in fluctuating conditions of temperature, pH and aw. Int J Food Microbiol 145, S96-S102.

Reynolds AE, Harrison MA, Rose-Morrow R, Lyon CE, 2001. Validation of dry cured ham process for control of pathogens. J Food Sci 66:1373–1379.

Tananuwong K, Chitsakun T, Tattiyakul J, 2012. Effects of high-pressure processing on inactivation of Salmonella Typhimurium, eating quality, and microstructure of raw chicken breast fillets. J of Food Sci 77(11):E321-7.

Tao Y, Sun D, Hogan E, Kelly AL, 2016. High-Pressure Processing of Foods: An Overview. In Da-Wen Sun, 2nd ed. Emerging Technologies for Food Processing. Academic Press, pp3-24.

Todd ECD, 2004. Microbiological safety standards and public health goals to reduce foodborne disease. Meat Sci 66:33–43.

Yuste J, Pla R, Mor-Mur M, 2000. Salmonella enteritidis and aerobic mesophiles in inoculated poultry sausages manufactured with high-pressure processing. Lett Appl Microbiol 31(5):374-7.

Zanardi E, Novelli E, Ghiretti GP, ChizzoliniR. 2000. Oxidative stability of lipids and cholesterol in salame Milano, coppa and Parma ham: dietary supplementation with vitamin E and oleic acid. Meat Science, 55(2): 169-175.

1.
Taddei R, Giacometti F, Bardasi L, Bonilauri P, Ramini M, Fonatana MC, Bassi P, Castagnini S, Ceredi F, Pelliconi MF, Serraino A, Tomasello F, Piva S, Mondo E, Merialdi G. Effect of production process and high-pressure processing on viability of <em>Salmonella</em> spp. in traditional Italian dry-cured coppa. Ital J Food Safety [Internet]. 2020 Aug. 19 [cited 2024 Mar. 28];9(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/8445

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