Arcobacter spp. in raw milk from vending machines in Piedmont and occurrence of virulence genes in isolates

  • Amaranta Traversa | amarantatraversa@gmail.com Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Silvia Gallina Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Francesca Martucci Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Cvetelina Boteva Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Elisa Baioni Epidemiology and Epidemiological Observatory complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Cristiana Maurella Epidemiology and Epidemiological Observatory complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Laura Chiavacci Epidemiology and Epidemiological Observatory complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Elisa Benvenuto Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Irene Ferrero Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Elena Ferrero Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Federica Giacometti Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, Italy.
  • Silvia Piva Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, Italy.
  • Francesco Chiesa Department of Veterinary Sciences, University of Torino, Grugliasco, Italy.
  • Daniela Manila Bianchi Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.
  • Andrea Serraino Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia, Italy.
  • Lucia Decastelli Food Control and Production Hygiene complex unit, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino, Italy.

Abstract

Arcobacter spp. has been recognized as an emerging foodborne pathogen and a hazard to human health. In the dairy chain, it has been isolated from different sources, nevertheless data on Arcobacter occurrence in raw milk provided by vending machines are few. This study aimed to identify potentially pathogenic Arcobacter spp. in raw milk intended for human consumption sold through vending machines located in Piedmont. In an 8-month period, 37 raw milk samples were collected from 24 dairy farms: 12 (32,4%) were collected directly in farm from bulk tank milk and 25 (67,6%) from vending machines. Eight (21,6%) out of the 37 milk samples and 7 (29,2%) out of the 24 dairy farms were positive for Arcobacter spp. by culture examination. Four (16%) out of the 25 samples from vending machines and 4 (33,3%) out of the 12 samples from bulk tank milk were positive. All 8 isolates were identified as A. butzleri both by MALDI-TOF MS and multiplex end-point PCR. According to the detection of virulence genes, a total of four Patho-types were highlighted: 5 isolates in P-type 1 and only one isolate for each of the P-types 2-3-4. A. butzleri isolates carrying encoding virulence factors genes were isolated from raw milk intended for human consumption: these findings strengthen the compulsory consumption after boiling as required by current legislation and suggest the need of enlarging the analytical investigations to other microorganisms not yet included in the food safety criteria.

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Published
2019-12-05
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Original Articles
Supporting Agencies
This project was funded by the Ministry of Health (Project Code IZS PLV 05/14 RC)
Keywords:
Arcobacter spp., Raw milk, Vending machine, Virulence genes
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How to Cite
Traversa, A., Gallina, S., Martucci, F., Boteva, C., Baioni, E., Maurella, C., Chiavacci, L., Benvenuto, E., Ferrero, I., Ferrero, E., Giacometti, F., Piva, S., Chiesa, F., Bianchi, D., Serraino, A., & Decastelli, L. (2019). Arcobacter spp. in raw milk from vending machines in Piedmont and occurrence of virulence genes in isolates. Italian Journal of Food Safety, 8(4). https://doi.org/10.4081/ijfs.2019.7859

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