Antimicrobial activity evaluation of pure compounds obtained from Pseudoalteromonas haloplanktis against Listeria monocytogenes: Preliminary results
https://doi.org/10.4081/ijfs.2022.10320
Accepted: 11 April 2022
HTML: 7
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
L. monocytogenes is a foodborne pathogen responsible for a serious disease with a high mortality rate, particularly in vulnerable consumers. Recently, the scientific community has shown increasing attention to the search for new natural molecules with antimicrobial activity, aimed at preventing the spread of foodborne diseases. Extremophilic microorganisms, typical of extreme temperature environments, are a valuable source of these molecules. The present work aimed to study the antibacterial activity of four pure compounds derived from a molecule, the pentadecanal, produced by the Antarctic bacterium Pseudoalteromonas haloplanktis, against two different pathotypes of L. monocytogenes. Growth assays were performed in 96-well polystyrene plates with serial dilutions of the tested compounds at different concentrations (0.6, 0.3, 0.15, 0.07 mg/mL). The plates were incubated at 37°C for 24 h, with a spectrophotometric reading at OD 600 nm. Preliminary results of this study showed that pentadecanal inhibits the growth of L. monocytogenes, with a MIC (Minimum Inhibitory Concentration) of 0.6 mg/mL. Acetal, carboxylic acid, and ester did not demonstrate antibacterial activity at the concentrations tested. These findings suggest the possibility of using pentadecanal as a natural antibacterial to improve safety standards along the food supply chain.
Ambrosio RL, Gratino L, Mirino S, Cocca E, Pollio A, Anastasio A, Palmieri G, Balestrieri M, Genovese A, Gogliettino M, 2020. The Bactericidal Activity of Protein Extracts from Loranthus europaeus Berries: A Natural Resource of Bioactive Compounds. Antibiotics 9:47. DOI: https://doi.org/10.3390/antibiotics9020047
Bisignano G, Laganà MG, Trombetta D, Arena S, Nostro A, Uccella N, Mazzanti G, Saija A, 2001. In vitro antibacterial activity of some aliphatic aldehydes from Olea europaea L. FEMS Microbiol Lett 198:9-13. DOI: https://doi.org/10.1111/j.1574-6968.2001.tb10611.x
Borucki MK, Peppin JD, White D, Loge F, Call DR, 2003. Variation in biofilm formation among strains of Listeria monocytogenes. Appl Environ Microbiol 69:7336-42. DOI: https://doi.org/10.1128/AEM.69.12.7336-7342.2003
Casillo A, Papa R, Ricciardelli A, Sannino F, Ziaco M, Tilotta M, Selan L, Marino G, Corsaro MM, Tutino ML, Artini M, Parrilli E, 2017. Anti-biofilm activity of along-chain fatty aldehyde from Antarctic Pseudoalteromonas haloplanktis TAC125 against Staphylococcus epidermidis biofilm. Front Cell Infect Microbiol 7:46. DOI: https://doi.org/10.3389/fcimb.2017.00046
Ceruso, M, Clement JA, Todd MJ, Zhang F, Huang Z, Anastasio A, Pepe T, Liu Y, 2020. The Inhibitory Effect of Plant Extracts on Growth of the Foodborne Pathogen, Listeria monocytogenes. Antibiotics 9:319. DOI: https://doi.org/10.3390/antibiotics9060319
Ceruso M, Liu Y, Gunther NW, Pepe T, Anastasio A, X Qi P, Tomasula MT, John A. Renye Jr JA, 2021. Anti-listerial activity of thermophilin 110 and pediocin in fermented milk and whey. Food Control 125:107941. DOI: https://doi.org/10.1016/j.foodcont.2021.107941
EFSA, ECDC, 2021. The European Union One Health 2019 Zoonoses Report. EFSA J 19:6406. DOI: https://doi.org/10.2903/j.efsa.2021.6406
EFSA, ECDC, 2021. The European Union One Health 2020 Zoonoses Report. EFSA J 19:6971. DOI: https://doi.org/10.2903/j.efsa.2021.6971
EFSA, 2018. Listeria monocytogenes contamination of ready-to-eat foods and the risk for human health in the EU. EFSA J16:e05134. DOI: https://doi.org/10.2903/j.efsa.2018.5134
Festa R, Ambrosio RL, Lamas A, Gratino L, Palmieri G, Franco CM, Cepeda A, Anastasio A, 2021. A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica. Foods 10:1372. DOI: https://doi.org/10.3390/foods10061372
Jamali H, Paydar M, Ismail S, Looi CY, Wong WF, Radmehr B, Abedini A, 2015. Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets. Bmc Microbiol 15:1-7. DOI: https://doi.org/10.1186/s12866-015-0476-7
Liu Y, Ceruso M, Gunther NW, Pepe T, Cortesi ML, Fratamico P, 2012. Construction of Listeria monocytogenes Mutants with In-Frame Deletions in Putative ATP-Binding Cassette (ABC) Transporters and Analysis of Their Growth under Stress Conditions. J Microb Biochem Technol 4:141-6. DOI: https://doi.org/10.4172/1948-5948.1000085
Liu Y, Ceruso M, Jiang Y, Datta AR, Pepe T, Anastasio A, Fratamico P, 2013. Construction of Listeria monocytogenes mutants with in-frame deletions in the phosphotransferase transport system (PTS) and analysis of their growth under stress conditions. J Food Sci 78:M1392-8. DOI: https://doi.org/10.1111/1750-3841.12181
Pacheco LA, Ethur EM, Sheibel T, Buhl B, Weber AC, Kauffmann C, Marchi MI, Freitas EM, Hoehne L, 2020. Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes. Braz J Biol 81:69-76. DOI: https://doi.org/10.1590/1519-6984.219889
Papa R, Parrilli E, Sannino F, Barbato G, Tutino ML, Artini M, Selan L, 2013. Anti-biofilm activity of the Antarctic marine bacterium Pseudoalteromonas haloplanktis TAC125. Res Microbiol 164:450–6. DOI: https://doi.org/10.1016/j.resmic.2013.01.010
Parrilli E, Papa R, Carillo S, Tilotta M, Casillo A, Sannino F, Cellini A, Artini M, Selan L, Corsaro MM, Tutino ML, 2015. Anti-biofilm activity of Pseudoal-teromonas haloplanktis TAC125 against Staphylococcus epidermidis biofilm: evidence of a signal molecule involvement. Int J Immunopathol Pharmacol 28:104–13. DOI: https://doi.org/10.1177/0394632015572751
Pepe T, De Dominicis R, Esposito G, Ventrone I, Fratamico PM, Cortesi ML, 2009. Detection of Campylobacter from poultry carcass skin samples at slaughter in Southern Italy. J Food Prot 72:1718-21. DOI: https://doi.org/10.4315/0362-028X-72.8.1718
Pieta L, Guzman F, Moya MLE, Campos FS, Frazzon APG, Frazzon, J, 2018. Comparative genomics suggests differences related to resistance and virulence between food-isolated Listeria monocytogenes serotypes 1/2a and 4b. EC Microbiol 14:755-66.
Ricciardelli A, Casillo A, Papa R, Monti DM, Imbimbo P, Vrenna G, Artini M, Selan L, Corsaro MM, Tutino ML, Parrilli E, 2018. Pentadecanal inspired molecules as new anti-biofilm agents against Staphylococcus epidermidis. Biofouling 34:1110-20. DOI: https://doi.org/10.1080/08927014.2018.1544246
Ricciardelli A, Casillo A, Corsaro MM, Tutino ML, Parrilli E, C van der Mei HC, 2020. Pentadecanal and pentadecanoic acid coatings reduce biofilm formation of Staphylococcus epidermidis on PDMS. Pathog Dis 78:ftaa012. DOI: https://doi.org/10.1093/femspd/ftaa012
Yuan W, Lee HW, Yuk HG, 2017. Antimicrobial efficacy of Cinnamomum javanicum plant extract against Listeria monocytogenes and its application potential with smoked salmon. Int J Food Microbiol 260:42-50. DOI: https://doi.org/10.1016/j.ijfoodmicro.2017.08.015
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Downloads
Citations
