Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning


Published: 24 May 2024
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Authors

  • Paul Dowling Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland.
  • Stephen Gargan Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland.
  • Margit Zweyer Department of Neonatology and Paediatric Intensive Care, Children’s Hospital, University of Bonn, Bonn, Germany; German Center for Neurodegenerative Diseases, Bonn, Germany.
  • Michael Henry National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland.
  • Paula Meleady National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland.
  • Dieter Swandulla Institute of Physiology, Medical Faculty, University of Bonn, Bonn, Germany. https://orcid.org/0000-0003-0923-7090
  • Kay Ohlendieck Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland. https://orcid.org/0000-0002-6266-4510

During the natural aging process, frailty is often associated with abnormal muscular performance. Although inter-individual differences exit, in most elderly the tissue mass and physiological functionality of voluntary muscles drastically decreases. In order to study age-related contractile decline, animal model research is of central importance in the field of biogerontology. Here we have analyzed wild type mouse muscle to establish a proteomic map of crude tissue extracts. Proteomics is an advanced and large-scale biochemical method that attempts to identify all accessible proteins in a given biological sample. It is a technology-driven approach that uses mass spectrometry for the characterization of individual protein species. Total protein extracts were used in this study in order to minimize the potential introduction of artefacts due to excess subcellular fractionation procedures. In this report, the proteomic survey of aged muscles has focused on organellar marker proteins, as well as proteins that are involved in cellular signaling, the regulation of ion homeostasis, bioenergetic metabolism and molecular chaperoning. Hence, this study has establish a proteomic reference map of a highly suitable model system for future aging research.


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Supporting Agencies

Kathleen Lonsdale Institute for Human Health Research, Science Foundation Ireland Infrastructure Award

Dowling, P., Gargan, S., Zweyer, M., Henry, M., Meleady, P., Swandulla, D., & Ohlendieck, K. (2024). Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning. European Journal of Translational Myology. https://doi.org/10.4081/ejtm.2024.12565

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