Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix


Published: 24 May 2024
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Authors

  • Paul Dowling Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland.
  • Stephen Gargan Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland.
  • Margit Zweyer Department of Neonatology and Paediatric Intensive Care, Children’s Hospital, University of Bonn, Bonn, Germany; German Center for Neurodegenerative Diseases, Bonn, Germany.
  • Michael Henry National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland. https://orcid.org/0000-0001-5312-4961
  • Paula Meleady National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland.
  • Dieter Swandulla Institute of Physiology, Medical Faculty, University of Bonn, Bonn, Germany. https://orcid.org/0000-0003-0923-7090
  • Kay Ohlendieck Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland; Kathleen Lonsdale Institute for Human Health Research, Maynooth University, Maynooth, Co. Kildare, Ireland. https://orcid.org/0000-0002-6266-4510

Sarcopenia of old age is characterized by the progressive loss of skeletal muscle mass and concomitant decrease in contractile strength. Age-related skeletal muscle dysfunctions play a key pathophysiological role in the frailty syndrome and can result in a drastically diminished quality of life in the elderly. Here we have used mass spectrometric analysis of the mouse hindlimb musculature to establish the muscle protein constellation at advanced age of a widely used sarcopenic animal model. Proteomic results were further analyzed by systems bioinformatics of voluntary muscles. In this report, the proteomic survey of aged muscles has focused on the expression patterns of proteins involved in the contraction-relaxation cycle, membrane cytoskeletal maintenance and the formation of the extracellular matrix. This includes proteomic markers of the fast versus slow phenotypes of myosin-containing thick filaments and actin-containing thin filaments, as well as proteins that are associated with the non-sarcomeric cytoskeleton and various matrisomal layers. The bioanalytical usefulness of the newly established reference map was demonstrated by the comparative screening of normal versus dystrophic muscles of old age, and findings were verified by immunoblot analysis.


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Supporting Agencies

Science Foundation Ireland, Kathleen Lonsdale Institute for Human Health Research

Dowling, P., Gargan, S., Zweyer, M., Henry, M., Meleady, P., Swandulla, D., & Ohlendieck, K. (2024). Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins located in the sarcomere, cytoskeleton and the extracellular matrix. European Journal of Translational Myology. https://doi.org/10.4081/ejtm.2024.12564

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