Development of a new method for extracting histamine from marine fish flesh using the salting-out technique


Submitted: 22 November 2023
Accepted: 2 February 2024
Published: 19 February 2024
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A simple and practical method was developed to extract histamine from fish products using sodium chloride (NaCl). After obtaining a saline extract from fish samples, histamine was derivatized by a condensation reaction with ortho-phthalaldehyde. Fluorescence intensity was measured by a fluorimeter. The first part of this work concerned a solid-liquid extraction tested with samples from the food analysis performance assessment scheme. The best histamine extraction yield (97%) was obtained using an extraction time of 4 minutes, a temperature of 40°C, and a NaCl/water ratio of 41% (w/w). The second part focused on a liquid-liquid extraction carried out on standard solutions of histamine (45, 90, and 180 mg/kg). The use of NaCl (41%) and trichloroacetic acid [(TCA) 10%] did not show any significant difference in extraction yield. The yield obtained was 99.15-100.1% for TCA (10%) and 98.65-99.45% for NaCl (41%). The validation criteria (repeatability and reproducibility) were checked by evaluating the reliability of the method. Extraction using NaCl has proven to be an interesting alternative method for the extraction of histamine from fish, as it is reliable, inexpensive, and less hazardous.


AOAC, 2012. Method 977.13. Histamine in seafood: fluorometric method. In: Association of Official Analytical Chemists 16th ed. AOAC International, Gaithersburg, USA.

Biji KB, Ravishankar CN, Venkateswarlu R, Mohan CO, Srinivasa TK, 2016. Biogenic amines in seafood: a review. J Food Sci Technol 53:2210-8.

Bilge B, Hüseyin G, 2015. Determination of biogenic amines in fish products. Food Sci Biotechnol 24:1907-13.

Dai JY, Sun YQ, Xiu ZL, 2014. Separation of bio-based chemicals from fermentation broths by salting-out extraction. Eng Life Sci 14:108-17

Endo S, Pfennigsdorff A, Goss KU, 2012. Salting-out effect in aqueous NaCl solutions: trends with size and polarity of solute molecules. Environ Sci Technol 46:1496-503.

FDA, 2020. Fish and fishery products - hazards and controls guidance 4th ed. Food and Drug Administration, Gainesville, United States, pp 113-52.

Felisiak K, Szymczak M, Kołakowski E, 2019. Identification of non-protein nitrogen compounds separated by CZE without derivatization from TCA extract from salted herring meat. J Food Compos Anal 77:108-14.

Fu H, Sun Y, Teng H, Zhang D, Xiu Z, 2015. Salting-out extraction of carboxylic acids. Sep Purif Technol 139:36-42.

Fu H, Wang X, Sun Y, Yan L, Shen J, Wang J, Shang Y, Xiu Z, 2017. Effects of salting-out and salting-out extraction on the separation of butyric acid. Sep Purif Technol 180:44-50.

Grozdanic ND, Calado MS, Kijercanin M, Serbanovic SP, 2014. Aqueous nicotine solutions: pH-measurements and salting-out effects – analysis of the effective Gibbs energies of hydration and ionic strengths of the solutions. J Serb Chem Soc 79:829-42.

Hyde AM, Zultanski SL, Waldman JH, Zhong YL, Shevlin M, Peng F, 2017. General principles and strategies for salting-out informed by the hofmeister series. Org Process Res Dev 21:1355-70.

Innocente N, Biasutti M, Padovese M, Moret S, 2007. Determination of biogenic amines in cheese using HPLC technique and direct derivatization of acid extract. Food Chem 101:1285-89.

ISO, 2019. Accuracy (trueness and precision) of measurement results and methods. Part 2: basic methods for determining the repeatability and reproducibility of a standard measurement method. ISO Norm 5725-2:2019. International Standardization Organization ed., Geneva, Switzerland.

Janči T, Valinger D, Gajdoš KJ, Mikac L, Vidaček S, Ivanda M, 2017. Determination of histamine in fish by surface enhanced raman spectroscopy using silver colloid SERS substrates. Food Chem 224:48-54.

Jiang J, Xiong YL, Chen J, 2010. pH shifting alters solubility characteristics and thermal stability of soy protein isolate and its globulin fractions in different pH, salt concentration, and temperature conditions. J Agric Food Chem 58:8035-42.

Lerke PA, Bell LD, 1976. A rapid fluorometric method for the determination of histamine in canned tuna fish. J Food Sci 41:1282-84.

Li FF, Li Q, Wu SG, Tan ZJ, 2016. Salting-out extraction of allicin from garlic (Allium sativum L.) based on ethanol/ammonium sulfate in laboratory and pilot scale. Food Chem 217:91-7.

Majors RE, 2009. Salting-out liquid-liquid extraction (SALLE). LCGC N Am J 27:526-33.

Novak P, Havlíček V, 2016. Protein extraction and precipitation. In: Ciborowski P, Silberring J (ed). Proteomic Profiling and analytical chemistry: the crossroads. Elsevier, New York, USA, pp 52-62.

Patange SB, Mukundan MK, Ashok KK, 2005. A simple and rapid method for colorimetric determination of histamine in fish flesh. Food Control 16:465-72.

Prester L, 2011. Biogenic amines in fish products and shellfish. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 28:1547-60.

Qixing J, Zhengran M, Shuoshuo W, Yanshun X, Fengyu T, Xueqin X, Wenshui X, 2014. Effect of temperature on protein compositional changes of big head carp (Aristichthys nobilis) muscle and exudates. Food Sci Technol Res 20:655-61.

Rachidi A, Oualit N, Touti A, Duflos G, Nôel L, Caillaud P, 2011. Proficiency test scheme for histamine analysis in fishery products in Morocco using the fluorometric method and comparison of results with those obtained with the HPLC. Int J Metrol Qual Eng 2:91-100.

Ramos RM, Valente IM, Rodrigues JA, 2014. Analysis of biogenic amines in wines by salting-out assisted liquid-liquid extraction and high-performance liquid chromatography with fluorimetric detection. Talanta 124:146-51.

Sadeghi R, Jahani F, 2012. Salting-in and salting-out of water-soluble polymers in aqueous salt solutions. J Phys Chem B 116:5234-41.

Sagratini G, Fernández FM, De Berardinis F, Font G, Vittori G, Mañes J, 2012. Simultaneous determination of eight underivatized biogenic amines in fish by solid phase extraction and liquid chromatography–tandem mass spectrometry. Food Chem 132:537-43.

Sazali NH, Alshishani A, Saad B, Chew KY, Chong MM, Miskam M, 2019. Salting-out assisted liquid-liquid extraction coupled with high-performance liquid chromatography for the determination of vitamin D3 in milk samples. Roy Soc Open Sci 6:190952.

Sparidans RW, Hoppe VS, Rood JJM, Schinkel AH, Schellens JHM, Beijnen JH, 2016. Liquid chromatography-tandem mass spectrometric assay for the tyrosine kinase inhibitor afatinib in mouse plasma using salting-out liquid-liquid extraction. J Chromatogr B Analyt Technol Biomed Life Sci 1012-1013:118-23.

Tang YQ, Weng N, 2013. Salting-out assisted liquid-liquid extraction for bioanalysis. Bioanalysis 5:1583-98.

Tomé LI, Pinho S, Jorge M, Gomes, Coutinho J, 2013. Salting-in with a salting-out agent: explaining the cation specific effects on the aqueous solubility of amino acids. J Phys Chem B 117:6116-28.

Tu X, Sun F, Wu S, Liu W, Gao Z, Huang S, Chen W, 2018. Comparison of salting-out and sugaring-out liquid-liquid extraction methods for the partition of 10 -hydroxy-2-decenoic acid in royal jelly and their co-extracted protein content. J Chromatogr B Analyt Technol Biomed Life Sci 1073:90-5.

Vogel AI, Tatchell AR, Furnis BS, Hannaford AJ, Smith PWG, 1996. vogel’s textbook of practical organic chemistry. Longman Scientific & Technical, London, UK.

Xu Q, Hong H, Yu W, Jiang X, Yan X, Wu J, 2019. Sodium chloride suppresses the bitterness of protein hydrolysates by decreasing hydrophobic interactions. J Food Sci 84:86-91.

Zarei M, Fazlara A, Najafzadeh H, Zolfaghar K F, 2014. Efficiency of different extraction solvents on recovery of histamine from fresh, frozen and canned fish. J Food Qual Haz Control 1:72-6.

Zarei M, Hosseini A, Shekarforoush S, 2009. Generation of bioluminescent Morganella morganii strain and its potential usage in determination of growth limits and histamine production. J Food Saf 29:190-206.

1.
Aoua C, Yacoubi B, Zekhnini A. Development of a new method for extracting histamine from marine fish flesh using the salting-out technique. Ital J Food Safety [Internet]. 2024 Feb. 19 [cited 2024 Apr. 16];13(1). Available from: https://www.pagepressjournals.org/ijfs/article/view/12117

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