Prevalence and antimicrobial resistance profiles of Salmonella spp. in poultry meat

Submitted: 30 December 2022
Accepted: 7 February 2023
Published: 8 June 2023
Abstract Views: 1369
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The spread of multidrug resistant (MDR) Salmonella strains, along the poultry supply chain, can represent a relevant threat to human health. This study aimed to evaluate the prevalence and antimicrobial resistance of Salmonella spp. isolated from poultry meat for human consumption. Between 2019 and 2021, 145 samples were analyzed according to ISO 6579-1:2017. The strains isolated were identified by using biochemical-enzymatic assays and serotyping, according to the Kauffmann-White-Le Minor scheme. The antibiotic susceptibility tests were determined using the Kirby-Bauer method. Forty Salmonella spp. strains were isolated and serotyping showed Salmonella Infantis to be predominant. 80% of the isolated strains were MDR and identified as S. Infantis. This study confirms the circulation of MDR Salmonella isolated from poultry meat and highlights the predominance of the S. Infantis serovar, which represents an emerging risk factor under the One Health holistic approach.

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Citations

CLSI, 2021. M100 – performance standards for antimicrobial susceptibility testing. 31st ed. Malvern (PA), USA: Clinical and Laboratory Standards Institute.
EFSA, 2021. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2018/2019. EFSA J 19:6490. DOI: https://doi.org/10.2903/j.efsa.2021.6490
EUCAST, 2017. Guideline for the detection of resistance mechanisms and specific resistances of clinical and/or epidemiological importance, version 2.0. Available from: https://www.eucast.org/resistance_mechanisms/. Accessed: 14 December 2021.
European Commission, 2005. Regulation of the European Parliament and of the Council of 15 November 2005 on microbiological criteria for foodstuffs, 2073/2005/CE. In: Official Journal, L 338, 22/12/2005.
European Medicine Agency, 2020. Categorisation of antibiotics used in animals promotes responsible use to protect public and animal health. Available online: https://www.ema.europa.eu/en/documents/press-release/categorisation-antibiotics-used-animals-promotes-responsible-use-protect-public-animal-health_en.pdf. Accessed: 19 October 2020.
Franco A, Leekitcharoenphon P, Feltrin F, Alba P, Cordaro G, Iurescia M, Tolli R, D'Incau M, Staffolani M, Di Giannatale E, Hendriksen RS, Battisti A, 2015. Emergence of a clonal lineage of multidrug-resistant esbl-producing salmonella infantis transmitted from broilers and broiler meat to humans in Italy between 2011 and 2014. PLoS One 10:e0144802. DOI: https://doi.org/10.1371/journal.pone.0144802
Gambino D, Gargano V, Butera G, Sciortino S, Pizzo M, Oliveri G, Cardamone C, Piraino C, Cassata G, Vicari D, Costa A, 2022. Food is reservoir of MDR Salmonella: prevalence of ESBLs profiles and resistance genes in strains isolated from food. Microorganisms 10:780. DOI: https://doi.org/10.3390/microorganisms10040780
Gargano V, Sciortino S, Gambino D, Costa A, Agozzino V, Reale S, Alduina R, Vicari D, 2021. Antibiotic susceptibility profile and tetracycline resistance genes detection in Salmonella spp. strains isolated from animals and food. Antibiotics (Basel) 10:809. DOI: https://doi.org/10.3390/antibiotics10070809
Hassena AB, Siala M, Guermazi S, Zormati S, Gdoura R, Sellami H, 2019. Occurrence and phenotypic and molecular characterization of antimicrobial resistance of Salmonella isolates from food in Tunisia. J Food Prot 82:1166-75. DOI: https://doi.org/10.4315/0362-028X.JFP-18-607
Hindermann D, Gopinath G, Chase H, Negrete F, Althaus D, Zurfluh K, Tall BD, Stephan R, Nüesch-Inderbinen M, 2017. Salmonella enterica serovar Infantis from food and human infections, Switzerland, 2010-2015: poultry-related multidrug resistant clones and an emerging ESBL producing clonal lineage. Front Microbiol 8:1322. DOI: https://doi.org/10.3389/fmicb.2017.01322
Holmes AH, Moore LS, Sundsfjord A, Steinbakk M, Regmi S, Karkey A, Guerin PJ, Piddock LJ, 2016. Understanding the mechanisms and drivers of antimicrobial resistance. Lancet 387:176-87. DOI: https://doi.org/10.1016/S0140-6736(15)00473-0
Hoque N, Mohiuddin RB, Khan MMH, Hannan A, Alam J, 2019. Outbreak of Salmonella in poultry of Bangladesh and possible remedy. J Adv Biotechonol Exp Ther 2:87-97. DOI: https://doi.org/10.5455/jabet.2019.d30
ISO, 2017. Microbiology of the food chain - horizontal method for the detection, enumeration and serotyping of salmonella - part 1: detection of salmonella spp. ISO norm 6579-1:2017. Geneva: International Organization for Standardization Publications.
Lauteri C, Festino AR, Conter M, Vergara A, 2022. Prevalence and antimicrobial resistance profile in Salmonella spp. isolates from swine food chain. Ital J Food Saf 11:9980. DOI: https://doi.org/10.4081/ijfs.2022.9980
Page SW, Gautier P, 2012. Use of antimicrobial agents in livestock. Rev Sci Tech 31:145-88. DOI: https://doi.org/10.20506/rst.31.1.2106
Parvin MS, Hasan MM, Ali MY, Chowdhury EH, Rahman MT, Islam MT, 2020. Prevalence and multidrug resistance pattern of Salmonella carrying extended-spectrum β-lactamase in frozen chicken meat in Bangladesh. J Food Prot 83:2107-21. DOI: https://doi.org/10.4315/JFP-20-172
Peruzy MF, Capuano F, Proroga YTR, Cristiano D, Carullo MR, Murru N, 2020. Antimicrobial susceptibility testing for Salmonella serovars isolated from food samples: five-year monitoring (2015-2019). Antibiotics (Basel) 9:365. DOI: https://doi.org/10.3390/antibiotics9070365
Proietti PC, Stefanetti V, Musa L, Zicavo A, Dionisi AM, Bellucci S, Mensa A, Menchetti L, Branciari R, Ortenzi R, Franciosini MP, 2020. Genetic profiles and antimicrobial resistance patterns of Salmonella Infantis strains isolated in Italy in the food chain of broiler meat production. Antibiotics (Basel) 9:814. DOI: https://doi.org/10.3390/antibiotics9110814
Sin M, Yoon S, Kim YB, Noh EB, Seo KW, Lee YJ, 2020. Molecular characteristics of antimicrobial resistance determinants and integrons in Salmonella isolated from chicken meat in Korea. J Appl Poult Res 29:502-14. DOI: https://doi.org/10.1016/j.japr.2019.12.010
Zhao X, Hu M, Zhang Q, Zhao C, Zhang Y, Li L, Qi J, Luo Y, Zhou D, Liu Y, 2020. Characterization of integrons and antimicrobial resistance in Salmonella from broilers in Shandong, China. Poult Sci 99:7046-54. DOI: https://doi.org/10.1016/j.psj.2020.09.071

How to Cite

1.
Castello A, Piraino C, Butera G, Alio V, Cardamone C, Oliveri G, Cascone G, Ciravolo C, Costa A. Prevalence and antimicrobial resistance profiles of <i>Salmonella</i> spp. in poultry meat. Ital J Food Safety [Internet]. 2023 Jun. 8 [cited 2024 Jul. 17];12(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/11135