Frequency and seasonality of viable Helicobacter pylori in drinking water in Dhamar Governorate, Yemen


Submitted: 9 September 2022
Accepted: 29 August 2023
Published: 6 September 2023
Abstract Views: 451
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Authors

Helicobacter pylori is an important and common bacterial pathogen in humans. The accumulated evidence of the existence of H. pylori in water from different environmental sources suggests a water-borne transmission route. This study aimed to investigate the occurrence of H. pylori in different water sources used by human populations in Dhamar Governorate, Yemen. 250 samples were randomly collected from the municipal water supply network, wells, and springs. The samples were processed, plated onto modified campy-blood agar, and incubated under microaerobic conditions for 4-10 days. Bacterial identification was based on morphological properties and biochemical tests. Bacteriological analysis showed that 9.6% and 13.2% of tap and surface water samples were contaminated with H. pylori, respectively. Despite a higher frequency in samples from rural areas, these were not significantly (p=0.068) more contaminated than the samples from urban areas. Regarding the seasonal variations of H. pylori detection, 85.71% of positive samples were detected in the late winter and spring seasons (February to May). To conclude, H. pylori transmission through water is likely to occur in Dhamar Governorate. Further prospective studies are highly recommended to provide further evidence and a clearer picture of H. pylori transmission.


Abadi ATB, Kusters JG, 2016. Management of Helicobacter pylori infections. BMC Gastroenterol 16:94.

Abdel-Latif MMM, Abouzied MM, 2016. Molecular mechanisms of natural honey against H. pylori infection via suppression of NF-κB and AP-1 activation in gastric epithelial cells. Arch Med Res 47:340-8.

Ahmed KS, Khan AA, Ahmed I, Tiwari SK, Habeeb A, Ahi JD, Abid Z, Ahmed N, Habibullah CM, 2007. Impact of household hygiene and water source on the prevalence and transmission of Helicobacter pylori: a South Indian perspective. Singapore Med J 48:543-9.

Almashhadany DA, 2018. Application of stool antigen test for monitoring Helicobacter pylori among human in Erbil Governorate, Kurdistan Region, Iraq. Int J Pharm Pharm Sci 10:49-53.

Almashhadany DA, 2020. Epidemiology of Helicobacter pylori among human at Erbil Governorate/Kurdistan Region/Iraq. World J Pharm Pharm Sci 9:435-47.

Almashhadany DA, Ismael LQ, Zaki AM, 2018. Seroprevalence of Helicobacter pylori among human in Erbil governorate, Kurdistan region, Iraq. Res J Life Sci Bioinform Pharm Chem Sci 4:268-80.

Almashhadany DA, Mayass SM, 2017. Incidence of Helicobacter pylori in food and water in Dhamar governorate/Yemen. Int J Curr Res 9:45320-6.

Almashhadany DA, Mayass SM, 2018. Prevalence of Helicobacter pylori in human in Dhamar Governorate/Yemen. J Med Pharm Sci 2. DOI: 10.26389/AJSRP.S101217.

Almashhadany DA, Mayas SM, Ali NL, 2022. Isolation and identification of Helicobacter pylori from raw chicken meat in Dhamar Governorate, Yemen. Ital J Food Saf 11:10220.

Almashhadany DA, Zefenkey ZF, Zaki AM, 2022. Dental risk factors associated with oral Helicobacter pylori infection: a cross-sectional study based on saliva antigen test. J Infect Dev Ctries 16:516-21.

Al-Sulami AA, Al-Edani TAA, Al-Abdula AA, 2012. Culture method and PCR for the detection of Helicobacter pylori in drinking water in Basrah Governorate Iraq. Gastroenterol Res Pract 2012:245167.

Ashbolt NJ, 2004. Microbial contamination of drinking water and disease outcomes in developing regions. Toxicology 198:229-38.

Azevedo NF, Guimaraes N, Figueiredo C, Keevil CW, Vieira MJ, 2007. A new model for the transmission of Helicobacter pylori: role of environmental reservoirs as gene pools to increase strain diversity. Crit Rev Microbiol 33:157-69.

Aziz RK, Khalifa MM, Sharaf RR, 2015. Contaminated water as a source of Helicobacter pylori infection: a review. J Adv Res 6:539-47.

Bellack NR, Koehoorn MW, MacNab YC, Morshed MG, 2006. A conceptual model of water’s role as a reservoir in Helicobacter pylori transmission: a review of the evidence. Epidemiol Infect 134:439-49.

Boehnke KF, Brewster RK, Sánchez BN, Valdivieso M, Bussalleu A, Guevara M, Saenz CG, Alva SO, Gil E, Xi C, 2018. An assessment of drinking water contamination with Helicobacter pylori in Lima, Peru. Helicobacter 23:e12462.

Castillo-Rojas G, Mazarí-Hiriart M, López-Vidal Y, 2004. Helicobacter pylori: focus on CagA and VacA major virulence factors. Salud Publica Mex 46:538-48.

Chen MC, Hu CT, Wang LY, Lin HH, Sheu BS, Kao AW, Cheng HC, Hunag SF, Chen TW, Lu CC, Wu JJ, 2005. The efficacy of Helicobacter pylori eradication related to CYP2C19 metabolism. Aliment Pharmacol Ther 22:274-5.

Chmiela M, Kupcinskas J, 2019. Review: pathogenesis of Helicobacter pylori infection. Helicobacter 24:e12638.

Domínguez-Bello M, Beker B, Guelrud M, 2002. Short report: socioeconomic and seasonal variations of Helicobacter pylori infection in patients in Venezuela. Am J Trop Med Hyg 66:49-51.

Doyle ME, 2012. Helicobacter spp. Food or Waterborne Pathogens? Available from: https://fri.wisc.edu/files/Briefs_File/FRI_Brief_Helicobacter_Feb2012.pdf.

Enroth H, Engstrand L, 1995. Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens. J Clin Microbiol 33:2162-5.

Hegarty JP, Dowd MT, Baker KH, 1999. Occurrence of Helicobacter pylori in surface water in the United States. J Appl Microbiol 87:697-701.

Hooi JKY, Lai WY, Ng WK, Suen MMY, Underwood FE, Tanyingoh D, Malfertheiner P, Graham DY, Wong VWS, Wu JCY, Chan FKL, Sung JJY, Kaplan GG, Ng SC, 2017. Global prevalence of Helicobacter pylori infection: systematic review and meta-analysis. Gastroenterology 153:420-9.

Hulten K, Han SW, Enroth H, Klein PD, Opekun AR, Gilman RH, Evans DG, Engstrand L, Graham DY, El-Zaatari FAK, 1996. Helicobacter pylori in the drinking water in Peru. Gastroenterology 110:1031-5.

Kayali S, Manfredi M, Gaiani F, Bianchi L, Bizzarri B, Leandro G, Mario F Di, De’angelis GL, 2018. Helicobacter pylori, transmission routes and recurrence of infection: state of the art. Acta Biomed 89:72-6.

Khalifa MM, Sharaf RR, Aziz RK, 2010. Helicobacter pylori: a poor man’s gut pathogen? Gut Pathog 2:2.

Khan A, Farooqui A, Kazmi SU, 2012. Presence of Helicobacter pylori in drinking water of Karachi, Pakistan. J Infect Dev Ctries 6:251-5.

Klein PD, Graham DY, Gaillour A, Opekun AR, Smith EO, 1991. Water source as risk factor for Helicobacter pylori infection in Peruvian children. Gastrointestinal physiology working group. Lancet 337:1503-6.

Kusters JG, Vliet AHM Van, Kuipers EJ, 2006. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev 19:449-90.

Momtaz H, Dabiri H, Souod N, Gholami M, 2014. Study of Helicobacter pylori genotype status in cows, sheep, goats and human beings. BMC Gastroenterol 14:61.

Moreno YP, JL A, González AM, 2007. Survival and viability of Helicobacter pylori after inoculation into chlorinated drinking water. Water Res 41:3490-6.

Niknam R, Seddigh M, Fattahi MR, Dehghanian A, Mahmoudi L, 2014. Prevalence of Helicobacter pylori in patients with dyspepsia. Jundishapur J Microbiol 7:e12676.

Paradowska A, Sieja A, Braksator M, 2010. Helicobacter pylori infection and oral cavity. Gastroenterologia Polska 17:423-6.

Plonka M, Targosz A, Brzozowski T, 2014. Can drinking water serve as a potential reservoir of Helicobacter pylori? Evidence for water contamination by Helicobacter pylori. In: Roesler BM. Trends Helicobacter pylori Infection. London, UK: IntechOpen.

Ranjbar R, Khamesipour F, Jonaidi-Jafari N, Rahimi E, 2016. Helicobacter pylori isolated from Iranian drinking water: vacA, cagA, iceA, oipA and babA2 genotype status and antimicrobial resistance properties. FEBS Open Bio 6:433-41.

Talaei R, Souod N, Momtaz H, Dabiri H, 2015. Milk of livestock as a possible transmission route of Helicobacter pylori infection. Gastroenterol Hepatol Bed Bench 8:30:S30-6.

Vesga FJ, Moreno Y, Ferrús MA, Campos C, Trespalacios AA, 2018. Detection of Helicobacter pylori in drinking water treatment plants in Bogotá, Colombia, using cultural and molecular techniques. Int J Hyg Environ Health 221:595-601.

Whale A, Said B, Owen B, Watson C, Drobniewski F, Nichols G, Lee J V, Weldon L, Edwards R, Lai S, 2003. Further studies on the Incidence of Mycobacterium avium Complex (MAC) in drinking water supplies (including the detection of Helicobacter pylori in water and biofilm samples). Available from: https://dwi-content.s3.eu-west-2.amazonaws.com/wp-content/uploads/2020/10/27110801/DWI70-2-146_mac1.pdf.

WHO, 2022. Drinking-water: key facts. Available from: https//www.who.int/news-room/fact-sheets/detail/drinking-water.

Yahaghi E, Khamesipour F, Mashayekhi F, Dehkordi FS, Sakhaei MH, Masoudimanesh M, Khameneie MK, 2014. Helicobacter pylori in vegetables and salads: genotyping and antimicrobial resistance properties. Biomed Res Int 2014:757941.

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Almashhadany DA, Mayas SM, Omar JA, Muslat TAM. Frequency and seasonality of viable <i>Helicobacter pylori</i> in drinking water in Dhamar Governorate, Yemen. Ital J Food Safety [Internet]. 2023 Sep. 6 [cited 2024 Jun. 13];12(3). Available from: https://www.pagepressjournals.org/ijfs/article/view/10855

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