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Molecular typing of Staphylococcus aureus isolate responsible for staphylococcal poisoning incident in homemade food

Guerrino Macori, Alberto Bellio, Daniela Manila Bianchi, Silvia Gallina, Daniela Adriano, Fabio Zuccon, Francesco Chiesa, Pier Luigi Acutis, Francesco Casalinuovo, Lucia Decastelli
  • Guerrino Macori
    Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy | guerrino.macori@izsto.it
  • Alberto Bellio
    Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy
  • Daniela Manila Bianchi
    Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy
  • Silvia Gallina
    Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy
  • Daniela Adriano
    Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy
  • Fabio Zuccon
    Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy
  • Francesco Chiesa
    Department of Animal Pathology, University of Turin, Grugliasco (TO), Italy
  • Pier Luigi Acutis
    Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy
  • Francesco Casalinuovo
    Institute for Experimental Veterinary Medicine of Southern Italy, Catanzaro, Italy
  • Lucia Decastelli
    Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin; Italian Reference Laboratory for Coagulase Positive Staphylococci including S. aureus, Institute for Experimental Veterinary Medicine of Piedmont, Liguria and Aosta Valley, Turin, Italy

Abstract

In October 2012, two persons fell ill with symptoms consistent with staphylococcal food poisoning after eating home-canned tuna fish and tomatoes. Laboratory investigation detected the enterotoxins in the home-canned tuna and molecular analysis of the isolated Staphylococcus aureus confirmed it carried toxin genes. Qualitative enzyme-linked immunosorbent assay and enzime linked fluorescent assay methods and quantitative assay identified the enterotoxins in the food leftovers, specifically staphylococcal enterotoxins type A (SEA) and D (SED), respectively 0.49 and 2.04 ng/g. The laboratory results are discussed considering the relation to the fish in oil, survival and heat resistance of S. aureus, and presumptive microbial contamination due to improper handling during home-canning procedures. This is the first reported cluster of foodborne illnesses due to staphylococcal enterotoxins in tuna in Italy. In this study, we reported cases described and analysed for their spa-type. Showing a high heterogeneity of isolates, spa-type t13252 is correlated in a node of the minimum spanning tree and it has never been reported as responsible for foodborne outbreak. This case underlines the importance of risk communication and dissemination of home-canning guidelines to reduce the incidence of foodborne outbreaks caused by homemade conserves.

Keywords

Staphylococcus aureus; Foodborne pathogen; Outbreak investigation; Molecular typing; Spa-typing

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Submitted: 2016-01-12 12:39:39
Published: 2016-06-03 14:38:39
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Copyright (c) 2016 Guerrino Macori, Alberto Bellio, Daniela Manila Bianchi, Silvia Gallina, Daniela Adriano, Fabio Zuccon, Francesco Chiesa, Pier Luigi Acutis, Francesco Casalinuovo, Lucia Decastelli

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