Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

Submitted: 15 November 2016
Accepted: 10 April 2017
Published: 18 May 2017
Abstract Views: 2267
PDF: 1091
HTML: 391
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Blooms of cyanobacteria are an increasingly frequent phenomenon in freshwater ecosystems worldwide as a result of eutrophication. Many species can produce hepatotoxins that cause severe health hazards to humans. The aim of this study was to identify the bloom forming cyanobacteria species by molecular methods and to amplify genes responsible for hepatotoxin biosynthesis from the environmental samples and isolated strains of cyanobacteria from Küçükçekmece Lagoon, Sapanca, İznik, Manyas and Taşkısı Lakes. A total of 10 bloom samples and 11 isolated strains were examined and Microcystis spp., Planktothrix spp., Nodularia spumigena, Anabaenopsis elenkinii, Sphaerospermopsis aphanizomenoides, Cylindrospermopsis raciborskii were identified. Hepatotoxin genes were detected in 60% of the bloom samples and 45% of the strains. Two Microcystis strains were obtained from Küçükçekmece Lagoon. While the strain assigned to Microcystis flosaquae was non-toxic, Microcystis aeruginosa strain produced microcystin. According to PCR results, the M. aeruginosa and Planktothrix agardhii bloom samples of Küçükçekmece Lagoon contained the microcystin synthetase gene E (mcyE) indicative of microcystin production, however, no microcystin was detected by HPLC. The mcyE gene was also found in Microcystis wesenbergii isolated from Taşkısı Lake, and in all Planktothrix rubescens bloom samples from Sapanca Lake. To our knowledge, this is the first detailed study for identifiying different toxic cyanobacteria species and their hepatotoxin production from several waterbodies in Turkey using molecular methods.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Supporting Agencies

Istanbul University, University of New South Wales, CYANOCOST

How to Cite

Köker, L., Akçaalan, R., Albay, M., & Neilan, B. A. (2017). Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey. Advances in Oceanography and Limnology, 8(1). https://doi.org/10.4081/aiol.2017.6394