Review Articles
Vol. 98 No. 2 (2025)
Therapeutic potential of Withania somnifera (Ashwagandha): a comprehensive review of its phytochemical, pharmacological, and clinical applications
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: 16 April 2025
Accepted: 13 November 2025
Accepted: 13 November 2025
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Withania somnifera, commonly known as Ashwagandha or “Indian ginseng”, is one of the most important medicinal plants in Ayurvedic tradition, historically used as a rejuvenating tonic (rasayana) to promote vitality, stress resilience, and longevity. In recent years, scientific interest in Ashwagandha has grown significantly, particularly for its potential applications in managing stress, chronic fatigue, cognitive decline, and various inflammatory and metabolic disorders. Several preclinical and clinical studies suggest that Withania somnifera exhibits adaptogenic, anti-inflammatory, antioxidant, immunomodulatory, and neuroprotective activities, primarily attributed to the presence of withanolides, alkaloids, and flavonoids. However, despite encouraging findings, much of the current evidence stems from small-scale studies with heterogeneous methodologies, variable dosages, and inconsistencies in the standardization of extracts. Moreover, in some cases, clinical efficacy appears to fall short of expectations, with outcomes not always reaching statistical significance compared to placebo. This review critically examines the pharmacological properties and proposed mechanisms of action of Withania somnifera's bioactive compounds, while highlighting both its therapeutic potential and the methodological limitations present in current research. The review was conducted using databases such as PubMed, ScienceDirect, HerbMed, and Google Scholar. Although Ashwagandha remains a promising candidate in the context of integrative medicine, larger and longer-term clinical trials are needed to confirm its effectiveness and to better define its safety profile.
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1. Singh N, Bhalla M, de Jager P, Gilca M. An overview on ashwagandha: arasayana of ayurveda. Afr. J. Tradit. Complement. Altern Med 2011;8:208–13. DOI: https://doi.org/10.4314/ajtcam.v8i5SS.9
2. Mikulska P, Malinowska M, Ignacyk M, et al. Ashwagandha (Withania somnifera) current research on the health-promoting activities: a narrative review. Pharmaceutics 2023;15:1057. DOI: https://doi.org/10.3390/pharmaceutics15041057
3. Langade D, Kanchi S, Salve J, et al. Efficacy and safety of ashwagandha (Withania somnifera) root extract in insomnia and anxiety: a double-blind, randomized, placebo-controlled study. Cureus 2019;11:5797. DOI: https://doi.org/10.7759/cureus.5797
4. Połumackanycz M, Forencewicz A, Wesołowski M, Viapiana A. Ashwagandha (Withania somnifera L.) the plant with proven health-promoting properties. Farm Pol 2020;76:442–7. DOI: https://doi.org/10.32383/farmpol/127824
5. Mirjalili MH, Moyano E, Bonfill M, et al. Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules 2009;14:2373–93. DOI: https://doi.org/10.3390/molecules14072373
6. John J. Therapeutic potential of Withania somnifera: a report on phyto-pharmacological properties. Int J Pharm Sci Res 2014;5:2131–48.
7. Spears AB, Cabey KA, Soumyanath A, Wright KM. Effects of Withania somnifera on stress and stress related neuropsychiatric disorders anxiety, depression and insomnia. Neuropharmaceutical Curr 2021;19:1468-95. DOI: https://doi.org/10.2174/1570159X19666210712151556
8. Majeed M, Nagabhushanam K, Mundkur L. A standardized ashwagandha root extract alleviates stress, anxiety, and improves quality of life in healthy adults by modulating stress hormones: results from a randomized, double-blind, placebo-controlled study. Medicine (Baltimore) 2023;102:e35521. DOI: https://doi.org/10.1097/MD.0000000000035521
9. Panossian AG, Efferth T, Shikov AN, et al. Evolution of the adaptogenic concept from traditional use to medical systems: pharmacology of stress and aging related diseases. Med Res Rev 2021;41:630–703. DOI: https://doi.org/10.1002/med.21743
10. Smith SJ, Lopresti AL, Fairchild TJ. Explore the efficacy and safety of a new standardized ashwagandha (Withania somnifera) root extract (Witholytin®) in adults suffering from high stress and fatigue in a randomized, double-blind, placebo-controlled study. J Psychopharmacy 2023;37:1091-104. DOI: https://doi.org/10.1177/02698811231200023
11. Lopresti AL, Smith SJ, Malvi H, Kodgule R. An investigation into the stress-relieving and pharmacological actions of an ashwagandha (Withania somnifera) extract: a randomized, double-blind, placebo-controlled study. Medicine (Baltimore) 2019;98:e17186. DOI: https://doi.org/10.1097/MD.0000000000017186
12. Wankhede S, Langade D, Joshi K, et al. Examining the effect of Withania somnifera supplementation on muscle strength and recovery: a randomized controlled trial. J Int Soc Sports Nutr 2015;12:43. DOI: https://doi.org/10.1186/s12970-015-0104-9
13. Tiwari S, Gupta SK, Pathak AK. A double-blind, randomized, placebo-controlled trial on the effect of ashwagandha (Withania somnifera dunal.) root extract in improving cardiorespiratory endurance and recovery in healthy athletic adults. J Ethnopharmacol 2021;272:113929. DOI: https://doi.org/10.1016/j.jep.2021.113929
14. Gregory J, Vengalasetti YV, Bredesen DE, Rao RV. Neuroprotective herbs for the management of alzheimer’s disease. Biomolecules 2021;11.4:543. DOI: https://doi.org/10.3390/biom11040543
15. Wiciński M, Fajkiel-Madajczyk A, Kurant Z, et al. Ashwagandha’s multifaceted effects on human health: impact on vascular endothelium, inflammation, lipid metabolism, and cardiovascular outcomes - a review. Nutrients 2024;16:2481. DOI: https://doi.org/10.3390/nu16152481
16. Salve J, Pate S, Debnath K, Langade D. Adaptogenic and anxiolytic effects of ashwagandha root extract in healthy adults: a double-blind, randomized, placebo-controlled clinical study. Cureus 2019;11:e6466. DOI: https://doi.org/10.7759/cureus.6466
17. Kushwaha S, Betsy A, Chawla P. Effect of ashwagandha (Withania somnifera) root powder supplementation in treatment of hypertension. Stud Ethno-Med 2012;6.2:111-15. DOI: https://doi.org/10.1080/09735070.2012.11886427
18. Singh M, Jayant K, Singh D. Withania somnifera (L.) Dunal (ashwagandha) for the possible therapeutics and clinical management of SARS-CoV-2 infection: plant-based drug discovery and targeted therapy. Front Cell Infect Microbiol 2022;12:933824. DOI: https://doi.org/10.3389/fcimb.2022.933824
19.Wiciński M, Fajkiel-Madajczyk A, Kurant Z, et al. Can ashwagandha benefit the endocrine system?- a review. Int J Mol Sci 2023;24:16513. DOI: https://doi.org/10.3390/ijms242216513
20. Sharma AK, Basu I, Singh S. Efficacy and safety of ashwagandha root extract in subclinical hypothyroid patients: a randomised, double-blind, placebo-controlled study. J Altern Complement Med 2018;24:243-48. DOI: https://doi.org/10.1089/acm.2017.0183
21. Chauhan S, Srivastava MK, Pathak AK. Effect of standardized root extract of ashwagandha (Withania somnifera) on well-being and sexual performance in adult males: A randomized controlled trial. Health Sci Rep 2022;5:e74. DOI: https://doi.org/10.1002/hsr2.741
22. Boroujeni, SN, Malamiri, FA, Bossaghzadeh F, et al. The most important medicinal plants affecting sperm and testosterone production: a systematic review. JBRA Assist Reprod 2022;26:522-30. DOI: https://doi.org/10.5935/1518-0557.20210108
23. Baghel K, Srivastava R. Photoperiod dependent expression of estrogen receptor alpha in testes of japanese quail: Involvement of Withania somnifera in apoptosis amelioration. Biochem Biophys Res Commun 2021;534:957-65. DOI: https://doi.org/10.1016/j.bbrc.2020.10.064
24. Narra K, Naik SK, Ghatge AS. A study of efficacy and safety of ashwagandha (Withania somnifera) lotion on facial skin in photoaged healthy adults. Cureus 2023;15:e36168. DOI: https://doi.org/10.7759/cureus.36168
25. Love A, Bjornsdottir TB, Olafsson S, et al. Low prevalence of hepatitis E in iceland: a seroepidemiological study. Scand J Gastroenterol 2018;53:293-96. DOI: https://doi.org/10.1080/00365521.2017.1420218
26. Bessone F, Hernandez N, Lucena MI, et al. The Latin American DILI registry experience: a successful ongoing collaborative strategic initiative. Int J Mol Sci 2016;17:313. DOI: https://doi.org/10.3390/ijms17030313
27. Björnsson HK, Björnsson ES, Avula B, et al. Ashwagandha-induced liver injury: a series of cases from Iceland and the U.S. Liver Int 2020;40:825-29. DOI: https://doi.org/10.1111/liv.14393
28. Siddiqui S, Ahmed N, Goswami M, et al. DNA damage by withanone as a potential cause of liver toxicity observed for herbal products of Withania somnifera (ashwagandha). Curr Res Toxicol 2021;2:72-81. DOI: https://doi.org/10.1016/j.crtox.2021.02.002
How to Cite
Therapeutic potential of Withania somnifera (Ashwagandha): a comprehensive review of its phytochemical, pharmacological, and clinical applications. (2025). Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 98(2). https://doi.org/10.4081/jbr.2025.13901
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