https://doi.org/10.4081/jbr.2019.7939
'Drink and sleep like a fish': goldfish as a behavior model to study pharmaceutical effects in freshwater ecosystems
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Published: 11 January 2019
Behavior is a mechanism through which organisms react to internal and external stimuli to best cope with challenges in an ever-changing environment. The study of animal behavior patterns in response to environmental stress/threats, is a relatively new and unexplored topic. The aim of this study is to offer a modest contribution in explaining the effects of pharmaceutical pollutants found in freshwater ecosystems, using the behavior patterns and physiology of Carassius auratus. Behavior changes were evaluated through swimming patterns, opercula and pectoral response, and rheological aggressivity. Animals were exposed for 5 weeks to water (as control), ethanol (EtOH, 0.25 and 1%, v/v), fluoxetine (FLX, 100 μg/L) and caffeine (CAF, 50 mg/L) and their short-term responses were recorded. The video has been analyzed using the open-sourced software program Track3D and EthoVision XT, which objectively quantified swimming and social behaviors. In all treatments, fish showed significantly (P<0.01) high level of stress, aggressivity and hyperactivity, compared to control. An interesting fact was that for each pollutant, fish exhibited different swimming patterns, from the normal one. These changes in the nervous system such as stressed behavior, irregular swimming patterns, hyperactivity and aggression, are consequences of pharmaceutical pollution in freshwater bodies and as such they can be used as suitable early physiological response biomarkers to environmental stress. Monitoring of altered behavior is a great early indicator of water pollution, which can easily be applied in the best aquaculture and fishery practices.
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Barry MJ. 2013. Effects of fluoxetine on the swimming and behavioral responses of the Arabian killifish. Ecotoxicology; 22(2):425-32. Doi: 10.1007/s10646-012-1036-7.
Dulawa SC, Holick KA, Gundersen B, Hen R. 2004. Effects of chronic fluoxetine in animal models of anxiety and depression. Neuropsychopharmacology; 29:1321–30.
Ebele A.J, Abdallah E.M.A., Harrad S. 2017. Pharmaceuticals and personal care products (PPCPs) in the freshwater aquatic environment. Emerging Contaminants; 3(1): 1-16.
Egan RJ, Bergner CL, Hart PC, Cachat JM, Canavello PR, Elegante MF, Elkhayat SI, Bartels BK, Tien AK, Tien DH, Mohnot S, Beeson E, Glasgow E, Amri H, Zukowska Z, Kalueff AV. 2009. Understanding behavioral and physiological phenotypes of stress and anxiety in zebrafish. Behavioral Brain Research; 205(1): 38–44. Doi: 10.1016/j.bbr.2009.06.022.
Fent, K., Weston, A.A., Caminada, D., 2006. Ecotoxicology of human pharmaceuticals. Aquatic Toxicology; 76: 122–159.
Gerlai R, Lahav M, Guo S, Rosenthal A. 2000. Drinks like a fish: Zebra fish (Danio rerio) as a behavior genetic model to study alcohol effects. Pharmacol. Biochem. Behav.; 67:773–782.
Gerlai R, Lee V, Blaser R. 2006. Effects of acute and chronic ethanol exposure on the behavior of adult zebrafish (Danio rerio). Pharmacol Biochem Behav.; 85: 752–761.
Lu Z., Xu Z., Buscher WJ. (2003). Acoustic response properties of lagena nerve fibers in sleeper Goby Dormitator Latiforns. Journal of Comparative Physiology: A. Neuroethology, Sensory, Neural and Behaviour Physiology; 89/ 12.
Malina E Hale, Ryan D. Day, Dean H Thorsen and Mark W Westneat. 2006. Pectoral fin coordination and gait transitions in steadily swimming juvenile reef fish. Journal of experimental biology; 209: 3708-3718.
Muir D., Simmons D., Wang X., Peart, T., Villella M., Miller J., Jim Sh. 2017. Bioaccumulation of pharmaceuticals and personal care product chemicals in fish exposed to wastewater effluent in an urban wetland. Scientific Reports; 7 (1) DOI: 10.1038/s41598-017-15462-x
Navarez V., Jhon F., Jimenez C., Claudio L. 2012. Pharmaceutical products in the environment: sources, effects and risks. Vitae; 19/1: 93-108.
Nikolaou A., Meric S., Fatta D. 2007. Occurrence patterns of pharmaceuticals in water and wastewater environments. Anal. Bioanal. Chem.; 387: 1225-1234.
Nuro A., Pine O., Shëngjergji D., Dama A., Napuce A., Borshi Xh., Muharemi E. 2016. Determination of some antibiotics in water samples using HPLC/DAD. Optime; 2: 65-75. ISSN 2220 – 461X.
Pannia E., Tran E., Rampersad M., Gerlai, R. 2014. Acute ethanol exposure induces behavioral differences in two zebrafish (Danio rerio) strains: A time course analysis. Behav. Brain Res.; 259: 174–185. Doi: [10.1016/j.bbr.2013.11.006]
Shrivastava Sh., Thakur U., Shrivastava, L. 2011. Behavioural Responses of Tilapia mossambica to Water Polluted with Fly Ash from Coal: A Laboratory Study. International Journal of Biology; 3/1: 153-160.
Simmons D. B. D., McCallum E. S., Balshine S., Chandramouli B., Cosgrove J., Sherry J. P. 2017. Reduced anxiety is associated with the accumulation of six serotonin reuptake inhibitors in wastewater treatment effluent exposed goldfish Carassius auratus. Scientific Reports; 7 (1) DOI: 10.1038/s41598-017-15989-z
Singer M., Oreschak K., Rhinehart Z., Robinson BD. 2016. Anxiolytic effects of fluoxetine and nicotine exposure on exploratory behavior in zebrafish. PeerJ; 4: e2352. Doi: 10.7717/peerj.2352
Stephen J. Walsh. (2004). Fish behaviour in exploted ecosystem; ICES journal of marine science: Journal du Conseil; 61 (7): 1030-1035; DOI: 10.1016/j.icesjms.2004.08.006(C).
Sudakov SK, Medvedeva OF, Rusakova IV, Figurina IB. 2001. Effect of short-term and chronic caffeine intake on rats with various anxiety level. Bull Exp Biol Med; 132:1177–9.
Tran S, Gerlai R. 2013. Time-course of behavioral changes induced by ethanol in zebrafish (Danio rerio) Behavioral Brain Research; 252: 204–213.
Zenki KM., Mussulini BHM., PachecoRico E., deOliveira DL., Rosemberg BD. 2014. Effects of ethanol and acetaldehyde in zebrafish brain structures: An in vitro approach on glutamate uptake and on toxicity-related parameters. Toxicology in Vitro; 28/5: 822-828.
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