Poster
Vol. 87 No. 1 (2014): Proceedings of the 85th SIBS Congress
https://doi.org/10.4081/jbr.2014.2138

Magnesium intracellular content and distribution map in drug-resistant and -sensitive whole cells

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Received: 21 November 2013
Published: 10 January 2014
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magnesium, XRFM, AFM, drug-resistance

Authors

Emil Malucelli
emil.malucelli@unibo.it
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Stefano Lagomarsino
Department of Physics, Institute of Chemical and Physical processess, La Sapienza University, Roma, Italy.
Lucia Merolle
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Chiara Marraccini
Department of Life Sciences, University of Modena and Reggio Emilia, Italy.
Azzurra Sargenti
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Concettina Cappadone
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Giovanna Farruggia
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Alessia Cedola
Institute of Photonics and Nanotechnology – CNR, Roma, Italy.
Michela Fratini
Institute of Photonics and Nanotechnology – CNR, Roma, Italy.
Andrea Notargiacomo
Institute of Photonics and Nanotechnology – CNR, Roma, Italy.
Stefano Iotti
National Institute of Biostructures and Biosystems, Roma, Italy.
Magnesium (Mg) plays crucial structural and regulatory roles within cells. Despite the extensive amount of data about the biochemistry of Mg, a complete picture of its regulation and cellular homeostasis is lacking. Thanks to recent improvements in third generation synchrotron X-ray sources, X-ray fluorescence microscopy (XRFM) is becoming a highly sensitive method for mapping elemental distributions in cells. XRFM maps the element content but not the concentration, which is a relevant variable in a biological context. We tackled this issue by combining XRFM with atomic force microscopy that was used to obtain morphological information of the sample. The aim of the present study was to compare the content and the distribution of Mg in drug-resistant and -sensitive tumor cell lines. Our data has shown a massive increase of Mg in LoVo drug-resistant cells. Moreover, the map of intracellular Mg showed marked differences in the pattern distribution between sensitive and resistant cells.

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Magnesium intracellular content and distribution map in drug-resistant and -sensitive whole cells. (2014). Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2138

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