Grape seed oil protects against phenylhydrazine-induced hemolysis in rats

Hemolytic anemia leads to mortality and morbidity if untreated. We evaluated the potential of Grape Seed Oil (GSO) against phenyl hydrazine (PHZ)-induced hemolytic anemia in rats. In this study, thirty male albino rats aging four weeks were randomly allocated to five groups with six rats per group: control (Group I), received a single dose of 0.1 mol/L citrate buffer. Group II, normal rats received GSO orally daily for 30 days. Groups (III-V): Rats received PHZ dissolved in dimethyl sulfoxide (DMSO) i.p, at dose of 10 mg/kg for 4 days. Rats in group IV and V were treated orally daily with GSO starting from day 6 for one month at 2 mL/kg body weight (bw) or 4mL/kg bw, respectively. The analysis of GSO by gas chromatography/mass spectrometry (GC/MS ) showed the presence of 1-Eicosanol, Behenic alcohol, 2-Piperidinone, N-[4-bromo-n-butyl]-, 2,6-Octadiene, Pentanoic acid, 10-undecenyl ester, 1-Hexacosanol and Undec-10-ynoic acid, dodecyl ester. In addition, different amount of fatty alcohol as  1-Heptacosanol. It was concluded that GSO ameliorates PHZ-induced anemia in rats. The effect of GSO was due to its phytochemical components which enhance antioxidant capacity and anti-inflammatory effect. In addition, GSO increased ferritin storage and transferrin. We recommend the isolation of the compounds and performing mechanistic studies of their mode of action.

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