Original Articles
Vol. 98 No. 2 (2025)
https://doi.org/10.4081/jbr.2025.13600
Impact of naringenin on the inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: 8 January 2025
Accepted: 26 May 2025
Published: 8 July 2025
Accepted: 26 May 2025
Published: 8 July 2025
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Protein glycation is one of the most serious issues in diabetes playing a critical role in the development of many cellular dysfunction as aging, cardiovascular diseases and neural disorders. This study investigated the impact of naringenin on the glycation rate of glomerular basement membrane protein in diabetic rats to prevent nephropathy as a complication of diabetes. Fifty male Wistar rats were sorted into 2 main groups, Gr I (normal; n=10) and Gr II (Diabetic, n=40). Diabetes was induced in rats by injection of a single dose of 60 mg/kg, i.p. streptozocin (STZ). Diabetic rats were allocated to Gr IIa: (not treated), Gr IIb: treated with naringenin (50 mg/kg body weight - bw), Gr IIc: Diabetic treated with naringenin (100 mg/kg bw), Gr IId: Diabetic treated with metformin (100 mg/kg bw). Serum was utilized for the determination of malondialdehyde (MDA), glycated hemoglobin (HA1c), fructosamine, and total antioxidant activity. Kidney tissue was utilized for the determination of oxidative stress and Advanced Glycated End products (AGEs). Obtained data showed that naringenin supplementation in diabetic rats reduced the levels of serum MDA (p=0.0111), HA1c (p=0.0112), fructosamine (p=0.011), and improved antioxidant capacity (p=0.032). In addition, it reduced the level of AGEs (p=0.0008), and enhanced antioxidant enzymes (p=0.01134) compared with untreated. Diabetic rats showed a significant elevation in inflammatory markers tumor necrosis factor- α (TNF-α) and interleukin-6 (IL-6), AGEs and decreased reduced glutathione (GSH), Superoxide Dismutase (SOD), Catalase (CAT) activity in kidney tissue versus control. The effect of naringenin was dose dependent, and improved these alterations versus untreated (p=0.0111). It was concluded that the inhibition of the formation of AGEs by naringenin in diabetics contributed to the protection against complications. Therefore, naringenin and its mechanisms of action is promising for the development of safety and effective new antidiabetic agent.
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Impact of naringenin on the inhibition of protein glycation in diabetic rats as a protection mechanism against nephropathy. (2025). Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 98(2). https://doi.org/10.4081/jbr.2025.13600
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