Thematic section: Natural compounds
Vol. 98 No. 2 (2025)
https://doi.org/10.4081/jbr.2025.12720
Cytotoxic activity of the crude extract and derived fractions from the sea anemone Telmatactis panamensis against cancer cell lines
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: 12 June 2024
Accepted: 26 November 2024
Published: 22 April 2025
Accepted: 26 November 2024
Published: 22 April 2025
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Sea anemones are considered a source of bioactive compounds with important pharmacological properties. In this study, we assessed the cytotoxic activity of Telmatactis panamensis and derived gel filtration fractions against the MCF-7 breast cancer and C6 rat glioma cell lines. The crude extract induced a concentrationand time-dependent response, reducing the viability of C6 cells from 38.76 to 1.90% with concentrations of 50–500 μg/mL at 24 h and from 11.81 to 0.73% at 48 h. In MCF-7 cells, the extract reduced viability from 61.71 to 20% and from 12.07 to 0.61% at 24 and 48 h, respectively. Fraction 1 provoked the highest cytotoxic activity in the C6 cell line, followed by fraction 3 and fraction 2. Lower sensitivity to the fractions was shown in MCF-7 cells, with only fraction 3 reducing viability by up to 50%. Both the extract and the fractions displayed low or no cytotoxicity in the normal breast cell line Hs 578Bst, suggesting that they present selectivity towards breast cancer cells over normal cells. The results support that T. panamensis represents a potential source for the discovery of biologically active compounds against tumor cell lines.
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Madio B, King GF, Undheim EAB. Sea anemone toxins: a structural overview. Mar Drugs 2019;17:325. DOI: https://doi.org/10.3390/md17060325
Frazão B, Vasconcelos V, Antunes A. Sea anemone (Cnidaria, Anthozoa, Actiniaria) toxins: an overview. Mar Drugs 2012;10:1812-51. DOI: https://doi.org/10.3390/md10081812
Lanio ME, Morera V, Alvarez C, et al. Purification and characterization of two hemolysins from Stichodactyla helianthus. Toxicon 2001;39:187-94. DOI: https://doi.org/10.1016/S0041-0101(00)00106-9
Marino A, Morabito R, La Spada G. Factors altering the haemolytic power of crude venom from Aiptasia mutabilis (Anthozoa) nematocysts. Comp Biochem Physiol A Mol Integr Physiol 2009;152:418-22. DOI: https://doi.org/10.1016/j.cbpa.2008.11.016
Cline EI, Wiebe LI, Young JD, Samuel J. Toxic effects of the novel protein UpI from the sea anemone Urticina piscivora. Pharmacol Res 1995;32:309-14. DOI: https://doi.org/10.1016/S1043-6618(05)80020-9
Díaz A, Tejuca M, Alvarez C, et al. Anticoagulant effect of total extract and some fractions obtained from the sea anemone Stichodactyla helianthus. Revista Iberoamericana de Trombosis y Homeostasis 1992;5:8-11.
Thangaraj S, Bragadeeswaran S, Suganthi K, Kumaran NS. Antimicrobial properties of sea anemone Stichodactyla mertensii and Stichodactyla gigantea from Mandapam coast of India. Asian Pac J Trop Biomed 2011;1:S43–6. DOI: https://doi.org/10.1016/S2221-1691(11)60120-2
Tejuca M, Anderluh G, Maček P, et al. Antiparasite activity of sea-anemone cytolysins on Giardia duodenalis and specific targeting with anti-Giardia antibodies. Int J Parasitol 1999;29:489-98. DOI: https://doi.org/10.1016/S0020-7519(98)00220-3
Soletti RC, de Faria GP, Vernal J, et al. Potentiation of anticancer-drug cytotoxicity by sea anemone pore-forming proteins in human glioblastoma cells. Anticancer Drugs 2008;19:517-25. DOI: https://doi.org/10.1097/CAD.0b013e3282faa704
Fedorov S, Dyshlovoy S, Monastyrnaya M, et al. The anticancer effects of actinoporin RTX-A from the sea anemone Heteractis crispa (=Radianthus macrodactylus). Toxicon 2010;55:811-7. DOI: https://doi.org/10.1016/j.toxicon.2009.11.016
Ramezanpour M, da Silva KB, Sanderson BJ. The effect of sea anemone (H. magnifica) venom on two human breast cancer lines: death by apoptosis. Cytotechnology 2014;66:845-52. DOI: https://doi.org/10.1007/s10616-013-9636-5
Tejuca M, Anderluh G, Dalla Serra M. Sea anemone cytolysins as toxic components of immunotoxins. Toxicon 2009;54:1206-14. DOI: https://doi.org/10.1016/j.toxicon.2009.02.025
Suput D. In vivo effects of cnidarian toxins and venoms. Toxicon 2009;54:1190-200. DOI: https://doi.org/10.1016/j.toxicon.2009.03.001
Bray F, Laversanne M, Sung H, et al. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2024;74:229-63. DOI: https://doi.org/10.3322/caac.21834
Nobili S, Lippi D, Witort E, et al. Natural compounds for cancer treatment and prevention. Pharmacol Res 2009;59:365-78. DOI: https://doi.org/10.1016/j.phrs.2009.01.017
Barreca M, Spanò V, Montalbano A, et al. Marine anticancer agents: An overview with a particular focus on their chemical classes. Mar Drugs 2020;18:619. DOI: https://doi.org/10.3390/md18120619
Patra S, Praharaj PP, Panigrahi DP, et al. Bioactive compounds from marine invertebrates as potent anticancer drugs: the possible pharmacophores modulating cell death pathways. Mol Biol Rep 2020;47:7209-28. DOI: https://doi.org/10.1007/s11033-020-05709-8
Avila AD, de Acosta CM, Lage A. A new immunotoxin built by linking a hemolytic toxin to a monoclonal antibody specific for immature T lymphocytes. Int J Cancer 1988;42:568-71. DOI: https://doi.org/10.1002/ijc.2910420417
Pederzolli C, Belmonte G, Serra MD, et al. Biochemical and cytotoxic properties of conjugates of transferrin with equinatoxin II, a cytolysin from a sea anemone. Bioconjug Chem 1995;6:166-73. DOI: https://doi.org/10.1021/bc00032a003
Soto C, Bergado G, Blanco R, et al. Sticholysin II-mediated cytotoxicity involves the activation of regulated intracellular responses that anticipates cell death. Biochimie 2018;148:18-35. DOI: https://doi.org/10.1016/j.biochi.2018.02.006
Acuña FH, González-Muñoz R, Garese A, Díaz-Ferguson E. First records of Anthopleura nigrescens (Verrill, 1928) and Telmatactis panamensis (Verrill, 1869) (Cnidaria, Anthozoa, Actiniaria) from Parque Nacional Coiba, Pacifc coast of Panama. Check List 2022;18:1141–6. DOI: https://doi.org/10.15560/18.5.1141
Prentis PJ, Pavasovic A, Norton RS. Sea anemones: quiet achievers in the field of peptide toxins. Toxins 2018;10:36. DOI: https://doi.org/10.3390/toxins10010036
Verrill, AE. Review of the polyps of the west coast of America. Trans Conn Acad Arts Sci 1869;1:377-558. DOI: https://doi.org/10.5962/bhl.title.38651
Kem WR, Parten B, Pennington MW, et al. Isolation, characterization, and amino acid sequence of a polypeptide neurotoxin occurring in the sea anemone Stichodactyla helianthus. Biochemistry 1989;28:3483-9. DOI: https://doi.org/10.1021/bi00434a050
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. DOI: https://doi.org/10.1006/abio.1976.9999
Chiba K, Kawakami K, Tohyama K. Simultaneous evaluation of cell viability by neutral red, MTT and crystal violet staining assays of the same cells. Toxicol In Vitro 1998;12:251-8. DOI: https://doi.org/10.1016/S0887-2333(97)00107-0
Repetto G, del Peso A, Zurita JL. Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc 2008;3:1125-31. DOI: https://doi.org/10.1038/nprot.2008.75
Librizzi M, Martino C, Mauro M, et al. Natural anticancer peptides from marine animal species: evidence from in vitro cell model systems. Cancers 2023;16:36. DOI: https://doi.org/10.3390/cancers16010036
Mariottini GL, Pane L. Cytotoxic and cytolytic cnidarian venoms. A review on health implications and possible therapeutic applications. Toxins 2013;6:108-51. DOI: https://doi.org/10.3390/toxins6010108
Horváth S. Cytotoxicity of drugs and diverse chemical agents to cell cultures. Toxicology 1980;16:59-66. DOI: https://doi.org/10.1016/0300-483X(80)90110-9
Ramezanpour M, Burke da Silva K, Sanderson B. Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms. J Venom Anim Toxins Incl Trop Dis 2012;18:157–63. DOI: https://doi.org/10.1590/S1678-91992012000200005
Monroy-Estrada HI, Chirino YI, Soria-Mercado IE, et al. Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells. J Venom Anim Toxins Incl Trop Dis 2013;19:12. DOI: https://doi.org/10.1186/1678-9199-19-12
Cutignano A, Nuzzo G, Ianora A, et al. Development and application of a novel SPE-method for bioassay-guided fractionation of marine extracts. Mar Drugs 2015;13:5736-49. DOI: https://doi.org/10.3390/md13095736
Riccio G, Martinez KA, Martín J, et al. Jellyfish as an alternative source of bioactive antiproliferative compounds. Mar Drugs 2022;20:350. DOI: https://doi.org/10.3390/md20060350
Moghadasi Z, Shahbazzadeh D, Jamili S, et al. Significant anticancer activity of a venom fraction derived from the Persian Gulf sea anemone, Stichodactyla haddoni. Iran J Pharm Res 2020;19:402-20.
Freshney RI. Culture of animal cells: a manual of basic technique and specialized. 6th ed. Hoboken, NJ: John Wiley & Sons; 2010. DOI: https://doi.org/10.1002/9780470649367
Hendrich AB, Michalak K. Lipids as a target for drugs modulating multidrug resistance of cancer cells. Curr Drug Targets 2003;4:23-30. DOI: https://doi.org/10.2174/1389450033347172
How to Cite
Cytotoxic activity of the crude extract and derived fractions from the sea anemone Telmatactis panamensis against cancer cell lines. (2025). Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 98(2). https://doi.org/10.4081/jbr.2025.12720
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