Field monitoring and genetic diversity of the large copper butterfly Lycaena dispar (Lepidoptera: Lycaenidae)

Submitted: February 21, 2024
Accepted: April 29, 2024
Published: May 21, 2024
Abstract Views: 122
PDF: 85
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The large copper butterfly Lycaena dispar (Haworth, 1803) (Lepidoptera: Lycaenidae), which is found across Europe and Asia, has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. The species has been infrequently observed outside its traditional range in the northwestern region, but no nationwide survey has yet been conducted. In addition, the population genetic data of L. dispar, which is informative to evaluate its vulnerability, remains unknown. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species. Mitochondrial cytochrome oxidase subunit I (COI) was also partially sequenced for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.



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IUCN (International Union for Conservation of Nature). The IUCN Red List of Threatened Species. Version 2023-1. 2023; Accessed on 10 January 2024.
NIBR (National Institute of Biological Resources). Red Data Book of Endangered Insects in Korea I. Paju, Korea: Nature and Ecology, Pub. Co.; 2012. pp 144-5.
Soulé ME. Conservation Biology: the science of scarcity and diversity. Sunderland, MA, USA: Sinauer Associates; 1986. pp 584.
Garner BA, Hoban S, Luikart G. IUCN Red List and the value of integrating genetics. Conserv Genet 2020;21:795-801. DOI:
Canteri E, Fordham DA, Li S, et al. IUCN Red List protects avian genetic diversity. Ecography 2021;44:1808-11. DOI:
Kim MJ, Cho Y, Wang AR, et al. Population genetic characterization of the black-veined white, Aporia crataegi (Lepidoptera: Pieridae), using novel microsatellite markers and mitochondrial DNA gene sequences. Conserv Genet 2020;21:359-71. DOI:
Kim MJ, Jeong SY, Kim S-S, et al. Population genetic characterization of the endangered dung beetle Copris tripartitus (Coleoptera: Scarabaeidae) using novel microsatellite markers. J Asia Pac Entomol 2022;25:101899. DOI:
Hoffmann AA, Sgro CM. Climate change and evolutionary adaptation. Nature 2011;470;479-85. DOI:
Lanfear R, Kokko H, Eyre-Walker A. Population size and the rate of evolution. Trends Ecol Evol 2014;29:33-41. DOI:
Gimenez DM. Lycaena dispar. The IUCN Red List of Threatened Species 1996. 1996; Accessed on 17 March 2023. DOI:
Pullin AS, Bálint Z, Balletto E, et al. The status, ecology and conservation of Lycaena dispar (Lycaenidae: Lycaenini) in Europe. Nota Lipid 1998;21:94-100.
Park SJ, Park DS, Kim NR. Habitat distributional change of the large copper, Lycaena dispar (Lepidoptera: Lycaenidae), on the Korean Peninsula: Studies along the Geum River. J Asia Pac Entomol 2014;17:245-50. DOI:
Lai BCG, Pullin AS. Phylogeography, genetic diversity and conservation of the large copper butterfly Lycaena dispar in Europe. J Insect Conserv 2004;8:27-36. DOI:
Martin LA, Pullin AS. Host-plant specialisation and habitat restriction in an endangered insect, Lycaena dispar batavus (Lepidoptera: Lycaenidae) I. Larval feeding and oviposition preferences. Eur J Entomol 2004;101:51-6. DOI:
Strausz M, Fiedler K, Franzén M, Wiemers M. Habitat and host plant use of the large copper butterfly Lycaena dispar in an urban environment. J Insect Conserv 2012;16:709-21. DOI:
Seok J. The distribution maps of butterflies in Korea. Seoul, Korea: Bojinjae; 1973. pp 517.
Kim S-S, Lee CM, Kwon T-S, et al. Korean butterfly atlas [1996–2011]. Research Note 461. Korea Forest Research Institute. Seoul, Korea: Korea Disabled Human Good Life Pub. Co.; 2012. pp 146-7.
Bae YJ, Lee BH. Human impacts on stream ecosystems and freshwater arthropods in Korea. Korean J Appl Entomol 2001;31:63-76.
Hajibabaei M, Singer GA, Hebert PD, Hickey DA. DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. Trends Genet 2007;23:167-72. DOI:
Wahlberg N, Saccheri I. The effects of Pleistocene glaciations on the phylogeography of Melitaea cinxia (Lepidoptera: Nymphalidae). Eur J Entomol 2007;104:675-84. DOI:
Jeong HC, Kim JA, Im HH, et al. Mitochondrial DNA sequence variation of the swallowtail butterfly, Papilio xuthus, and the cabbage butterfly, Pieris rapae. Biochem Genet 2009;47:165-78. DOI:
Jeong JS, Kim MJ, Park JS, et al. Tracing the invasion characteristics of the yellow-legged hornet, Vespa velutina nigrithorax (Hymenoptera: Vespidae), in Korea using newly detected variable mitochondrial DNA sequences. J Asia Pac Entomol 2021;24:135-47. DOI:
Hebert PDN, Penton EH, Burns J, et al. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly, Astraptes fulgerator. PNAS 2004;101:14812-7. DOI:
Swofford DL. PAUP* Phylogenetic Analysis Using Parsimony (*and Other Method) Version 4. 10. Sunderland, MA, USA: Sinauer Associates; 2002.
Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 2015;32:268-74. DOI:
Dinca V, Dapporto L, Somervuo P, et al. High resolution DNA barcode library for European butterflies reveals continental patterns of mitochondrial genetic diversity. Commun Biol 2021;4:315. DOI:
Minh BQ, Nguyen MAT, von Haeseler A. Ultrafast approximation for phylogenetic bootstrap. Mol Biol Evol 2013;30:1188-95. DOI:
Guindon S, Dufayard JF, Lefort V, et al. New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. Syst Biol 2010;59:307-21. DOI:
Leigh JW, Bryant D. POPART: full-feature software for haplotype network construction. Methods Ecol Evol 2015;6:1110-6. DOI:
Nei M. Molecular evolutionary genetics. Columbia University Press. New York, NY, USA: 1987. DOI:
Excoffier L, Lischer HEL. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 2010;10:564-7. DOI:
Corander J, Tang J. Bayesian analysis of population structure based on linked molecular information. Math Biosci 2007;205:19-31. DOI:
Bink FA. Acid stress in Rumex hydrolapathum (Polygonaceae) and its influence on the phytophage Lycaena dispar (Lepidoptera; Lycaenidae). Oecologia 1986;70:447-51. DOI:
Hume L, Cavers PB. Geographic variation in a widespread perennial weed, Rumex crispus. The relative amounts of genetic and environmentally induced variation among populations. Canad J Bot 1982;60:1928-37. DOI:
MOLIT (Ministry of Land Infrastructure and Transport). 4 Rivers Guide. 2010; Accessed 7 February 2024.
Im RY, Kim JY, Choi JY, et al. Changes of river morphology in the mid-lower part of Nakdong River basin after the 4 Large River Project, South Korea. Korean J Ecology and Environment 2015;48:188-94. DOI:
Jeong SY, Kim MJ, Park JS, et al. Population genetic characterization of the endangered silver skipper, Leptalina unicolor (Lepidoptera: Hesperiidae), using novel microsatellite markers and mitochondrial DNA gene sequences. J Asia Pac Entomol 2020;23:781-90. DOI:
Neigel JE, Avise JC. Application of a random walk model to geographic distributions of animal mitochondrial DNA variation. Genetics 1993;135:1209-20. DOI:
Watterson GA, Guess HA. Is the most frequent allele the oldest? Theor Popul Biol 1977;11:141-60. DOI:

Supporting Agencies

National Institute of Biological Resources

How to Cite

Park, J. S., Lee, S. H., Pyo, J.-Y., Jeong, H. C., Kim, S.-S., & Kim, I. (2024). Field monitoring and genetic diversity of the large copper butterfly <i>Lycaena dispar</i> (Lepidoptera: Lycaenidae). Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 97(1).