Wound healing and analgesic effects of Brocchia cinerea essential oil in experimental animals
https://doi.org/10.4081/jbr.2023.11346
Accepted: July 18, 2023
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
This study aimed to identify the main components of the essential oil extracted from Brocchia cinerea (Delile) Vis. via hydrodistillation and investigate its in vivo wound healing and analgesic properties. Thujone, santolina triene, camphor, and 1,8-cineole were among the compounds detected. Wounds were induced in mice and treated with essential oil, which resulted in accelerated wound healing and repair through topical application (88.1±1.1%). The study also evaluated the analgesic activity of the essential oil by administering intraperitoneal injections of acetic acid to mice. The results showed that B. cinerea essential oil at a dose of 400 mg/kg strongly inhibited pain, with a pain inhibition percentage of 95.5%. These findings indicate that the essential oil of B. cinerea has potential as a source of bioactive compounds that may have synergistic effects. Based on these results, the use of B. cinerea for therapeutic purposes in preventing pain and promoting wound healing is supported. These findings highlight the potential of B. cinerea in paving the way for future research aimed at the development of clinically valuable products.
Barkaoui M, Katiri A, Boubaker H, Msanda F. Ethnobotanical survey of medicinal plants used in the traditional treatment of diabetes in Chtouka Ait Baha and Tiznit (Western Anti-Atlas), Morocco. J Ethnopharmacol 2017;198:338-50. DOI: https://doi.org/10.1016/j.jep.2017.01.023
Ben Haj Yahia I, Jaouadi R, Trimech R, et al. Variation of chemical composition and antioxidant activity of essential oils of Mentha x rotundifolia (L.) Huds. (Lamiaceae) collected from different bioclimatic areas of Tunisia. Biochem Syst Ecol 2019;84:8-16. DOI: https://doi.org/10.1016/j.bse.2019.03.001
El-Hilaly J, Hmammouchi M, Lyoussi B. Ethnobotanical studies and economic evaluation of medicinal plants in Taounate province (Northern Morocco). J Ethnopharmacol 2003;86:149-58. DOI: https://doi.org/10.1016/S0378-8741(03)00012-6
Jamila F, Mostafa E. Ethnobotanical survey of medicinal plants used by people in Oriental Morocco to manage various ailments. J Ethnopharmacol 2014;154:76-87. DOI: https://doi.org/10.1016/j.jep.2014.03.016
Silalahi M, Nisyawat. The ethnobotanical study of edible and medicinal plants in the home garden of Batak Karo sub-ethnic in North Sumatra, Indonesia. Biodiversitas 2018;19:229-38. DOI: https://doi.org/10.13057/biodiv/d190131
Mrabti HN, Jaradat N, Kachmar MR, et al. Integrative herbal treatments of diabetes in Beni Mellal region of Morocco. J Integr Med 2019;17:93-9. DOI: https://doi.org/10.1016/j.joim.2019.01.001
Markouk M, Redwane A, Lazrek HB, et al. Antibacterial activity of Cotula cinerea extracts. Fitoterapia 1999;70:314-16. DOI: https://doi.org/10.1016/S0367-326X(99)00042-8
Djellouli M, Benmehdi H, Mammeri S, et al. Chemical constituents in the essential oil of the endemic plant Cotula cinerea (Del.) from the southwest of Algeria. Asian Pac J Trop Biomed 2015;5:870-3. DOI: https://doi.org/10.1016/j.apjtb.2015.06.007
Lakhdar M. Traditional uses, phytochemistry and biological activities of Cotula cinerea Del: A review. Trop J Pharm Res 2018;17:365-73. DOI: https://doi.org/10.4314/tjpr.v17i2.24
Adekenov SM. Sesquiterpene lactones from plants of the family Asteraceae in the Kazakhstan flora and their biological activity. Chem Nat Compd 1995;31:21-5. DOI: https://doi.org/10.1007/BF01167564
Ghouti D, Lazouni HA, Moussaoui A, Chabane Sari D. Chemical profile, in vitro antibacterial and antioxidant activities of Juniperus phoenicea L. and Cotula cinerea (Del.) essential oils from Southwestern Algeria. Phytothérapie 2018,16:S74-S83. DOI: https://doi.org/10.3166/phyto-2018-0061
Larbi BAM, Naima B, Elsharkawy ER, Salah N. Phytochemical characterization, in-vitro cytotoxic and antibacterial activity of Cotula cinerea (Delile) Vis essential oil. J Nat Remedies 2018;18. DOI: https://doi.org/10.18311/jnr/2018/22380
Guaouguaou F, Ahl Bebaha MA, Yadlapalli S, et al. Structural characterization of bioactive compounds in Cotula cinerea extracts by ultra-high-performance liquid chromatography with photodiode array and high-resolution time offlight mass spectrometry detectors (UHPLC-PDA-ESI-ToF/HRMS). Rapid Commun Mass Spectrom 2019;34:e8695. DOI: https://doi.org/10.1002/rcm.8695
Atef C, Boualem M, Cherif MM, et al. Chemical composition and antimicrobial activity of essential oils in Xerophytic plant Cotula cinerea Del (Asteraceae) during two stages of development: Flowering and fruiting. J Appl Pharm Sci 2015;5:29-34. DOI: https://doi.org/10.7324/JAPS.2015.50306
De Moura FBR, Ferreira BA, Deconte SR, et al. Wound healing activity of the hydroethanolic extract of the leaves of Maytenus ilicifolia Mart. Ex Reis. J Tradit Complement Med2021;11:446-56. DOI: https://doi.org/10.1016/j.jtcme.2021.03.003
Hilan C, Bouaoun D, Aoun J, et al. Antimicrobial properties and toxicity by LD50 determination of an essential oil of Prangosa sperula Boissier. Phytothérapie 2009;7:8-14. DOI: https://doi.org/10.1007/s10298-008-0357-4
Collier HO, Dinneen LC, Johnson CA, Schneider C. The abdominal construction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol Chemother 1968;32:295-310. DOI: https://doi.org/10.1111/j.1476-5381.1968.tb00973.x
Ekhlil B, Mohamed G, Satrani B, et al. Chemical quality, antibacterial and antifungal activities of Cotula cinerea essential oil from South Morocco. Environ Sci An Indian J 2016;12:209-16.
El Bouzidi L, Abbad A, Fattarsi K, et al. Chemical composition and anticandidal properties of the essential oil isolated from aerial parts of Cotula cinerea: A rare and threatened medicinal plant in Morocco. Nat Prod Commun 2011;6:1491-4. DOI: https://doi.org/10.1177/1934578X1100601021
Elsharkawy ER, Ed-Dra A, Alghanem S, Abdallah EM. Comparative studies of chemical compostion, antimicrobial and antioxidant activity of essential oil of some species from genus Artemisia. J Nat Remedies 2018;18:10-20. DOI: https://doi.org/10.18311/jnr/2018/20052
Ramos YJ, Machado D de B, Queiroz GA de, et al. Chemical composition of the essential oils of circadian rhythm and of different vegetative parts from Piper mollicomum Kunth - A medicinal plant from Brazil. Biochem Syst Ecol 2020;92:104-16. DOI: https://doi.org/10.1016/j.bse.2020.104116
Harkat-Madouri L, Asma B, Madani K, et al. Chemical composition, antibacterial and antioxidant activities of essential oil of Eucalyptus globulus from Algeria. Ind Crops Prod 2015;78:148-53. DOI: https://doi.org/10.1016/j.indcrop.2015.10.015
Barreto RS, Albuquerque-Júnior RL, Araújo AA, et al. A systematic review of the wound-healing effects of monoterpenes and iridoid derivatives. Molecules 2014;19:846-62. DOI: https://doi.org/10.3390/molecules19010846
Villegas LF, Marçalo A, Martin J et al. Epi-α-Bisbolol is the wound-healing principle of Peperomia. galioides: Investigation of the in vivo wound-healing activity of related terpenoids. J Nat Prod 2001;64:1357-9. DOI: https://doi.org/10.1021/np0102859
Dagne E, Bisrat D, Alemayehu M, Worku T. Essential oils of twelve Eucalyptus species from Ethiopia. J Essent Oil Res 2000;12:467-70. DOI: https://doi.org/10.1080/10412905.2000.9699567
Khalil Z, Pearce AL, Satkunanathan N, et al. Regulation of wheal and flare by tea tree oil: Complementary human and rodent studies. J Invest Dermatol 2004;123:683-90. DOI: https://doi.org/10.1111/j.0022-202X.2004.23407.x
Kawata J, Kameda M, Miyazawa M. Cyclooxygenase-2 inhibitory effects of monoterpenoids with a p-methane skeleton. Int J Essent Oil Ther 2008;2:145-8.
Hassan SB, Gali-Muhtasib H, Göransson H, Larsson R. Alpha terpineol: A potential anticancer agent which acts through suppressing NF-kappaB signalling. Anticancer Res 2010;30:1911-9.
Held S, Schieberle P, Somoza V. Characterization of alpha-terpineol as an anti-inflammatory component of orange juice by in vitro studies using oral buccal cells. J Agric Food Chem 2007;55:8040-6. DOI: https://doi.org/10.1021/jf071691m
Oliveira MG, Marques RB, De Santana MF, et al. α-terpineol reduces mechanical hypernociception and inflammatory response. Basic Clin Pharmacol Toxicol 2012;111:120-5. DOI: https://doi.org/10.1111/j.1742-7843.2012.00875.x
Park SN, Lim YK, Freire MO, et al. Antimicrobial effect of linalool and α-terpineol against periodontopathic and cariogenic bacteria. Anaerobe 2012;18:369-72. DOI: https://doi.org/10.1016/j.anaerobe.2012.04.001
Park MJ, Gwak KS, Yang I, et al. Effect of citral, eugenol, nerolidol and α-terpineol on the ultrastructural changes of Trichophyton mentagrophytes. Fitoterapia 2009;80:290-6. DOI: https://doi.org/10.1016/j.fitote.2009.03.007
Ehrnhöfer-Ressler MM, Fricke K, Pignitter M, et al. Identification of 1, 8-cineole, borneol, camphor, and thujone as anti-inflammatory compounds in a Salvia officinalis L. infusion using human gingival fibroblasts. J Agric Food Chem 2013;61:3451-9. DOI: https://doi.org/10.1021/jf305472t
Juergens UR. Anti-inflammatory properties of the monoterpene 1.8-cineole: Current evidence for co-medication in inflammatory airway diseases. Drug Res (Stuttg) 2014;64:638-46. DOI: https://doi.org/10.1055/s-0034-1372609
Juergens LJ, Racké K, Tuleta I, et al. Anti-inflammatory effects of 1,8-cineole (eucalyptol) improve glucocorticoid effects in vitro: A novel approach of steroid-sparing add-on therapy for COPD and asthma? Synergy 2017;5:1-8. DOI: https://doi.org/10.1016/j.synres.2017.08.001
Bicchi C, Rubiolo P, Ballero M, et al. HIV-1 inhibiting activity of the essential oil Ridolfia segetum and Oenanthe crocata plant medicine. J Chem and Mat Res 2009;75:1331-5. DOI: https://doi.org/10.1055/s-0029-1185546
Elsharkawy ER. Anticancer effect and seasonal variation in oil constituents of Santolina chamaecyparissus. Chem and Mat Res 2014;6:2224-3224.
Miller JA, Thompson PA, Hakim IA, et al. d-Limonene: a bioactive food component from citrus and evidence for a potential role in breast cancer prevention and treatment. Oncol Rev 2011;5:31-42. DOI: https://doi.org/10.4081/oncol.2011.14
Radulović NS, Genčić MS, Stojanović NM, et al. Toxic essential oils. Part V: Behaviour modulating and toxic properties of thujones and thujone-containing essential oils of Salvia officinalis L., Artemisia absinthium L., Thuja occidentalis L. and Tanacetum vulgare L. Food Chem Toxicol 2017;105:355-69. DOI: https://doi.org/10.1016/j.fct.2017.04.044
Sasikala P, Ganesan S, Jayaseelan T, et al. Phytochemicals and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Taraxacum officinale (L). J Drug Deliv Ther 2019;9:90-4. DOI: https://doi.org/10.22270/jddt.v9i1.2175
Sökmen G, Vandar-Ünlu M, Polissiou D, et al. Antimicrobial activity of essential oil and methanol extracts of Achillea sintenisii Hub. Mor. (Asteraceae). Phytother Res 2003;7:1005-10. DOI: https://doi.org/10.1002/ptr.1274
Martins MDR, Arantes S, Candeias F, et al. Antioxidant, antimicrobial and toxicological properties of Schinus molle L. essential oils. J Ethnopharmacol 2014;151:485-92. DOI: https://doi.org/10.1016/j.jep.2013.10.063
Chlif PN, Bouymajane A, Oulad El Majdoub Y, et al. Phenolic compounds, in vivo anti-inflammatory, analgesic and antipyretic activities of the aqueous extracts from fresh and dry aerial parts of Brocchia cinerea (Vis.). J Pharm Biomed Anal 2022;213:114695. DOI: https://doi.org/10.1016/j.jpba.2022.114695
Prabhu VV, Nalini G, Chidambaranathan N, Kisan NS. Evaluation of anti-inflammatory and analgesic activity of Tridax procumbens Linn against formalin, acetic acid and CFA induced pain models. Int J Pharm Pharm Sci 2011;3:126-30.
Copyright (c) 2023 the Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Downloads
Citations
