Gut microbiota shift of spangled emperor under pollution stress

  • Othman Baothman Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah; Microbial Toxins and Natural Products Centre, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Salem A-Zahrani Department of Biochemistry, Faculty of Science, Tabuk University, Tabuk, Saudi Arabia.
  • Hasan Al-Talhi | halttalhi@kau.edu.sa Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah; Microbial Toxins and Natural Products Centre, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia. https://orcid.org/0000-0003-1441-7942

Abstract

Hepatic antioxidant enzymes as oxidative stress biomarkers were investigated and correlated with the identified dominant gut microbial phyla. The results showed that while the antioxidant enzymes, Superoxide Dismutase (SOD), and Catalase (CAT) levels were reduced in the polluted PO site, significant elevation (*P ≥ 0.05) was observed at the clean reference CR site indicating negative correlation to pollution stress. On the other hand, among five significant bacterial genera, Lactobacillus and Vagococcus showed a positive relationship to the oxidative pollution stress between PO and CR sites. Diversity and bacterial richness had been observed in the PO site compared to the CR site. As a result, 429,346 sequences were obtained from the pooling of 20 samples identified into 10 phyla and 79 genera in which Firmicutes was dominant in both PO and CR sites. The number of common OTUs was 221 for both CR and PO samples. The results revealed that under the stressed environmental state, the homo-lactic Vagococcus genus is dominant over the hetero-lactic Lactobacillus, which uses less energy in the derived process.

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References

Nayak SK. Role of gastrointestinal microbiota in fish. Aquacult Res 2010;41:1553-73. DOI: https://doi.org/10.1111/j.1365-2109.2010.02546.x

Xia JH, Lin G, Fu GH, et al. The intestinal microbiome of fish under starvation. BMC Genomics 2014;15:266. DOI: https://doi.org/10.1186/1471-2164-15-266

Martínez-Álvarez RM, Morales AE, Sanz A. Antioxidant defenses in fish: biotic and abiotic factors. Rev Fish Biol Fisheries 2005;15:75-88. DOI: https://doi.org/10.1007/s11160-005-7846-4

Xu XH, Zhang YQ, Yan BL, et al. Immunological and histological responses to sulfide in the crab Charybdis japonica. Aquatic Toxicol 2014;150:144-50. DOI: https://doi.org/10.1016/j.aquatox.2014.03.006

Lawrence RA, Burk RF. Species, tissue and subcellular distribution of non Se-dependent glutathione peroxidase activity. J Nutr 1978;108:211-5. DOI: https://doi.org/10.1093/jn/108.2.211

Gomez GD, Balcazar JL. A review on the interactions between gut microbiota and innate immunity of fish. Fems Immunol Med Microbiol 2008;52:145-54. DOI: https://doi.org/10.1111/j.1574-695X.2007.00343.x

Yan M, Li Z, Xiong B, Zhu J. Effects of salinity on food intake, growth, and survival of pufferfish (Fugu obscurus). J Appl Ichthyol 2004;20:146-9. DOI: https://doi.org/10.1046/j.1439-0426.2003.00512.x

Pedrajas J, Peinado J, Lopez-Barea J. Oxidative stress in fish exposed to model xenobiotics. Oxidatively modified forms of Cu, Zn-superoxide dismutase as potential biomarkers. Chemico-Biolog Interact 1995;98:267-82. DOI: https://doi.org/10.1016/0009-2797(95)03651-2

Ali AA, Elazein EM, Alian MA. Investigation of heavy metals pollution in water, sediment and fish at Red Sea-Jeddah Coast-KSA at two different locations. J Appl Environ Biol Sci 2011;1:630-7.

Basaham A. Re-evaluation of the impact of sewage disposal on coastal sediments of the southern Corniche, Jeddah, Saudi Arabia. Marine Sci 2009;20. DOI: https://doi.org/10.4197/Mar.20-1.8

S Souza Md, Pinto FGdS, Fruet TK, et al. Water quality indicators for environmental and resistance profile of Escherichia coli strains isolated in Rio Cascavel, Paraná, Brazil. Engenharia Agrícola 2014;34:352-62. DOI: https://doi.org/10.1590/S0100-69162014000200016

Apha A. WPCF, Standard methods for the examination of water and wastewater. American Public Health Association, Washington, DC: 1995.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6

Foyer CH, Halliwell B. The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 1976;133:21-5. DOI: https://doi.org/10.1007/BF00386001

Rao M. Cellular detoxifying mechanisms determine the age dependent injury in tropical trees exposed to SO2. J Plant Physiol 1992;140:733-40. DOI: https://doi.org/10.1016/S0176-1617(11)81031-X

Aebi H. Catalase in vitro. Methods in enzymology. 105: Elsevier; 1984: p. 121-6. DOI: https://doi.org/10.1016/S0076-6879(84)05016-3

Kei S. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clinica Chimica Acta 1978;90:37-43. DOI: https://doi.org/10.1016/0009-8981(78)90081-5

Beyer Jr WF, Fridovich I. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Analytical Biochem 1987;161:559-66. DOI: https://doi.org/10.1016/0003-2697(87)90489-1

Almar M, Otero L, Santos C, Gallego JG. Liver glutathione content and glutathione‐dependent enzymes of two species of freshwater fish as bioindicators of chemical pollution. J Environ Sci Health Part B 1998;33:769-83. DOI: https://doi.org/10.1080/03601239809373177

Collier TK, Varanasi U. Hepatic activities of xenobiotic metabolizing enzymes and biliary levels of xenobiotics in English sole (Parophrys vetulus) exposed to environmental contaminants. Arch Environ Contamination Toxicol 1991;20:462-73. DOI: https://doi.org/10.1007/BF01065834

Stephensen E, Svavarsson J, Sturve J, et al. Biochemical indicators of pollution exposure in shorthorn sculpin (Myoxocephalus scorpius), caught in four harbours on the southwest coast of Iceland. Aquatic Toxicol 2000;48:431-42. DOI: https://doi.org/10.1016/S0166-445X(99)00062-4

Huang D, Zhang Y, Song G, et al. Contaminants-induced oxidative damage on the carp Cyprinus carpio collected from the upper Yellow River, China. Environ Monitoring Assessment 2007;128:483-8. DOI: https://doi.org/10.1007/s10661-006-9341-3

Van der Oost R, Lopes S, Komen H, et al. Assessment of environmental quality and inland water pollution using biomarker responses in caged carp (Cyprinus carpio): use of a bioactivation: detoxication ratio as a biotransformation index (BTI). Marine Environ Res 1998;46:315-9. DOI: https://doi.org/10.1016/S0141-1136(97)00096-2

Gilbert JA, Dupont CL. Microbial metagenomics: beyond the genome. Ann Rev Mar Sci 2011;3:347-71. DOI: https://doi.org/10.1146/annurev-marine-120709-142811

Ye L, Amberg J, Chapman D, et al. Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish. ISME J 2014;8:541. DOI: https://doi.org/10.1038/ismej.2013.181

Spor A, Koren O, Ley R. Unravelling the effects of the environment and host genotype on the gut microbiome. Nature Rev Microbiol 2011;9:279. DOI: https://doi.org/10.1038/nrmicro2540

Roeselers G, Mittge EK, Stephens WZ, et al. Evidence for a core gut microbiota in the zebrafish. ISME J 2011;5:1595. DOI: https://doi.org/10.1038/ismej.2011.38

Sugita H, Tokuyama K, Deguchi Y. The intestinal microflora of carp Cyprinus carpio, grass carp Ctenopharyngodon idella and tilapia Sarotherodon niloticus. Bull Jap Soc Sci Fish/Nissuishi 1985;51:1325-9. DOI: https://doi.org/10.2331/suisan.51.1325

Kandler O. Carbohydrate metabolism in lactic acid bacteria. Antonie van Leeuwenhoek 1983;49:209-24. DOI: https://doi.org/10.1007/BF00399499

Kandler O, Weiss N. Bergey’s manual of systematic bacteriology, Vol. 2 (PHA Sneath, NS Mair, and ME Sharpe, eds.). Williams and Wilkins, Baltimore. 1986:1209.

Omolo KM. Characterisation of Carbamate Degrading Aerobic Bacteria Isolated from Soils of Selected Horticultural Farms in Rift Valley and Central Kenya. Jomo Kenyatta University of Agriculture and Technology [Master Thesis]; 2013.

Gao J, Bao H-Y, Xin M-X, et al. Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31. J Zhejiang Univ Science B 2006;7:186-92. DOI: https://doi.org/10.1631/jzus.2006.B0186

Teles YV, de Castro LM, Junior ÉS, et al. Potential of Bacterial Isolates from a Stream in Manaus-Amazon to Bioremediate Chromium-Contaminated Environments. Water, Air, Soil Pollut 2018;229:266. DOI: https://doi.org/10.1007/s11270-018-3903-1

Published
2021-05-03
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Original Articles
Keywords:
Microbiota, Lethrinus nebulosus, antioxidant enzyme, homofermentative
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How to Cite
Baothman, O., A-Zahrani , S., & Al-Talhi, H. (2021). Gut microbiota shift of spangled emperor under pollution stress. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 94(1). https://doi.org/10.4081/jbr.2021.9519