Antibacterial and anti-inflammatory effects of lime (citrus aurantifolia) peel extract in mice balb/c induced salmonella typhi


Lime (Citrus aurantifolia) is a traditional plant that is widely used as antibacterial. This study to prove the effect of lime peel extract (LPE) on the growth of bacterial colonization of S. Typhi medited by activity of IL-6. True experimental pre-post test design, mice were divided into; LPE 510 mg/kgbw, LPE 750 mg/kgbw, positive and negative control. The examination was carried out 3 times, the 5th day before the intervention, the 10th day after the intervention and the 30th day after maintenance. Intervention LPE for 5 days can decreased the number of S. Typhi colonies, even maintenance for 20 days after the intervention showed no bacterial growth . IL-6 pro-inflammatory cytokine activity increased on examination day 5 after S.Typhi injection and decreased after intervention on day 10, significantly different between pre and post at all groups except negative controls (p=0.15). The speed of decrease in IL-6 levels was greatest at the LPE 750 mg/kgbw (velocity=-5.64%). LPE decreased serum levels of IL-6 and inhibit the growth of S. Typhi colony in mice Balb/c. LPE have potential as antibacterial and anti-inflammatory.



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1. Roka G, Pandaya S, Ferdous MR, et al. Susceptibility to patterns of ciprofloxacin among nalidixic acid-resistant salmonella isolates collected in Banepa, Nepal from enteric fever patients. J Biol Res - Boll della Soc Ital di Biol Sper. 2016;89:47–50.
2. Rani N, Bharti S, Krishnamurthy B, et al. Pharmacological properties and therapeutic potential of naringenin: a citrus flavonoid of pharmaceutical promise. Curr Pharm Des. 2016;22:4341–59.
3. Pathan R khan, Gali PR, Pathan P, In vitro antimicrobial activity of citrus aurantifolia and its phytochemical screening. Asian Pacific J Trop Dis. 2012;2:S328–3.
4. Loizzo MR, Tundis R, Bonesi M, et al. Evaluation of citrus aurantifolia peel and leaves extracts for their chemical composition, antioxidant and anti-cholinesterase activities. J Sci Food Agric. 2012;92:2960–7.
5. Lemes RS, Alves CCF, Estevam EBB, et al. Chemical composition and antibacterial activity of essential oils from citrus aurantifolia leaves and fruit peel against oral pathogenic bacteria. An Acad Bras Cienc. 2018;90:1285–92.
6. Tao X, Sun X, Xu L, et al. Total flavonoids from rosa laevigata michx fruit ameliorates hepatic ischemia/reperfusion injury through inhibition of oxidative stress and inflammation in rats. Nutrients. 2016;8:418.
7. Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005;26:343–56.
8. Abbas AK, Lichtman AH. Celluler and moleculer immunology. 8th ed. Philadelphia: W.B. Saunders Company; 2014.
9. Harley, John & Prescott L. Laboratory exercises in microbiology. 50th ed. Lab Exerc Microbiol:The McGraw-Hill Companies; 2002.
10. Sandoval-Montemayor NE, García A, Elizondo-Treviño E, et al. Chemical composition of hexane extract of citrus aurantifolia and anti-mycobacterium tuberculosis activity of some of its constituents. Molecules. 2012;17:11173–84.
11. Kwambana-Adams B, Darboe S, Nabwera H, et al. Salmonella infections in the Gambia, 2005–2015. Clin Infect Dis. 2015;61:S354–62.
12. Kaarthikeyan G, Balakrishnan A, Jayakumar ND. The link between the genetic polymorphisms of the innate immune signaling molecular factors with periodontitis. J Biol Res - Boll della Soc Ital di Biol Sper. 2018;91:53–5.
13. Pérez-Cano F, Massot-Cladera M, Rodríguez-Lagunas M, Castell M. Flavonoids affect host-microbiota crosstalk through tlr modulation. Antioxidants. 2014;3:649–70.
14. Ghasemi K, Sciences SA, Ghasemi Y, Ebrahimzadeh MA. Antioxidant activity , phenol and flavonoid contents of 13 Citrus species peels and tissues. Pak J Pharm Sci. 2009;22: 277-81.
15. Tejada S, Pinya S, Martorell M, et al. Potential anti-inflammatory effects of hesperidin from the genus citrus. Curr Med Chem. 2018;25:4929–45.
16. Amorim JL, Simas DLR, Pinheiro MMG, et al. Anti-inflammatory properties and chemical characterization of the essential oils of four citrus species. PLoS One. 2016;11:e0153643.
Original Articles
Lime peel extract, Citrus aurantifolia, Interleukin 6, antibacterial, anti-inflammatory
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How to Cite
Kasim, V. N., Hatta, M., Natzir, R., Hadju, V., Hala, Y., Budu, B., Alam, G., As’ad, S., Febriza, A., & Idrus, H. H. (2020). Antibacterial and anti-inflammatory effects of lime (citrus aurantifolia) peel extract in mice balb/c induced salmonella typhi. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale.

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