The effect of Citrullus colocynthis extracts on Streptococcus mutans, Candida albicans, normal gingival fibroblast and breast cancer cells


Submitted: April 3, 2019
Accepted: May 29, 2019
Published: June 21, 2019
Abstract Views: 1903
PDF: 929
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Authors

  • Hossein Seifi Kafshgari Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran, Islamic Republic of.
  • Mohsen Yazdanian Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran, Islamic Republic of.
  • Reza Ranjbar Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran, Islamic Republic of.
  • Elaheh Tahmasebi Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran, Islamic Republic of.
  • Seyed Rahim Ghazi Mirsaeed Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran, Islamic Republic of.
  • Hamid Tebyanian Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran, Islamic Republic of.
  • Mohammad Ali Ebrahimzadeh Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of.
  • Hamid Reza Goli Department of Medical Microbiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran, Islamic Republic of.

Tooth decay is one of the most common chronic diseases in humans. The aim of this study was to investigate the inhibitory effects of Citrullus colocynthis extracts on the growth of Streptococcus mutans and Candida albicans and their cytotoxic effects on normal gingival fibroblast cells and breast cancer cells. The aqueous and alcoholic extracts of C. colocynthis fruit were evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, minimum inhibitory concentration (MICs) and minimal bactericidal concentration (MBCs)/minimal fungicidal concentration (MFCs) tests. The MICs and MBCs/MFCs were obtained from the aqueous extract (MIC 0.37 mg/mL and MBCs 1.5 mg/mL against S. mutans and MIC 0.37 mg/mL and MFCs 3.0 mg/mL against C. albicans) and ethanolic extract (MIC 0.75 mg/mL and MBCs 1.5 mg/mL against S. mutans and MIC 3.0 mg/mL and MFCs 12.0 mg/mL against C. albicans). The growth of S. mutans and C. albicans were effectively inhibited by extracts of C. colocynthis. The LC50 values of C. colocynthis on HGF1-PI cells were 4589.19 μg/mL and 3933.84 μg/mL by aqueous and ethanolic extracts, respectively. The LC50 values of C. colocynthis on MCF-7 cells were 4589.19 μg/mL and 3933.84 μg/mL by aqueous and ethanolic extracts, respectively. The extracts of C. colocynthis significantly decreased the growth of breast cancer and normal gingival fibroblast cells. The results of the study showed that the extracts may be used to treat oral mucosal diseases and prevent dental caries but future research is needed.


Supporting Agencies

Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran

Seifi Kafshgari, H., Yazdanian, M., Ranjbar, R., Tahmasebi, E., Mirsaeed, S. R. G., Tebyanian, H., Ebrahimzadeh, M. A., & Goli, H. R. (2019). The effect of Citrullus colocynthis extracts on Streptococcus mutans, Candida albicans, normal gingival fibroblast and breast cancer cells. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 92(1). https://doi.org/10.4081/jbr.2019.8201

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