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Effect of cadmium on anion exchange capability through Band 3 protein in human erythrocytes

Rossana Morabito, Alessia Remigante, Benedetta Arcuri, Angela Marino, Marco Giammanco, Giuseppina La Spada, Angela Marino
  • Rossana Morabito
    Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy
  • Alessia Remigante
    Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy
  • Benedetta Arcuri
    Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy
  • Angela Marino
    Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy
  • Marco Giammanco
    Department of Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy
  • Giuseppina La Spada
    Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy

Abstract

The efficiency of Band 3 protein, mediating HCO3-/Cl- exchange across erythrocytes membrane, is reduced by oxidative stress. The aim of the present study was to verify whether Band 3 protein efficiency is compromised by treatment with Cadmium (Cd2+), an extremely toxic heavy metal known to interfere with antioxidant enzymes, energy metabolism, gene expression and cell membranes. To this end, the rate constant for SO4= uptake through Band 3 protein (accounting for velocity of anion exchange) was measured along with membrane –SH groups, Malonyldialdehyde (MDA) and Band 3 protein expression levels in Cd2+ -treated human erythrocytes (300 µM, 1 mM). Our results show that Cd2+ reduced the rate constant for SO4= uptake, with a significant increase in MDA levels at both concentrations and with a reduction in –SH groups observed after 1 mM Cd2+ treatment, whereas Band 3 protein expression levels were unchanged in both conditions. In conclusion: i) Cd2+ reduces Band 3 protein efficiency via different mechanisms depending on metal concentration and with unchanged expression levels; ii) the assessment of Band 3 protein anion exchange capability is a good tool to assay the impact of heavy metals on cell homeostasis and, possibly, useful for diagnosis and monitoring of devalopment of Cd2+ toxicity-related pathologies.

Keywords

Cadmium; Oxidative stress; SO4= uptake; Band 3 protein; Erythrocyte.

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Submitted: 2017-11-27 14:58:33
Published: 2018-05-15 12:22:58
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Copyright (c) 2018 Rossana Morabito, Alessia Remigante, Benedetta Arcuri, Angela Marino, Marco Giammanco, Giuseppina La Spada, Angela Marino

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