Effects of bosentan on collagen type I synthesis on in vitro culture of scleroderma skin fibroblasts

  • S. Soldano
  • P. Montagna
  • R. Brizzolara
  • B. Villaggio
  • C. Ferrone
  • A. Parodi
  • M. Cutolo | mcutolo@unige.it

Abstract

The present study evaluated the effects of a non-selective endothelin (ETA/B) receptors antagonist, on collagen type I (COLI) synthesis on in vitro culture of scleroderma (SSc) skin fibroblasts (Fb). Fb were obtained from skin biopsies of 6 female SSc patients (mean age 64. 1±6 years), after informed consent and Ethical Committee Approval. Cells were treated with endothelin-I [ET-I, 100nM] for 24 and 48 hrs, pre-treated for I hr with ETA/B receptors antagonist [10nM] alone or followed by ET-I for 24 and 48 hrs. Untreated Fb were used as controls. Immunocytochemistry and western blot analysis were performed to evaluate COLI synthesis. ET-I increased COLI synthesis both at 24 and 48 hrs when compared to controls. ETA/B receptor antagonost blocks the increased COLI synthesis ET-I-mediated both at 24 and 48 hrs vs. ET-I. Results showed that ET-I receptors blockage by ETA/B receptors antagonist might prevent the excessive synthesis of COLI, supporting its positive action in the management of skin fibrosis.

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Published
2011-01-30
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Abstract Book
Keywords:
endothelin-1, ETa/ETb receptor antagonist, fibrosis, systemic sclerosis
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How to Cite
Soldano, S., Montagna, P., Brizzolara, R., Villaggio, B., Ferrone, C., Parodi, A., & Cutolo, M. (2011). Effects of bosentan on collagen type I synthesis on in vitro culture of scleroderma skin fibroblasts. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 84(1). https://doi.org/10.4081/jbr.2011.4687