05 | Stimulation of muscle stem cells using side-streams from animal and plant production

N.T. Solberg¹, J. Matic¹, G.I. Borge², K. Aabye², V. Høst¹, S.G. Wubshet¹, Mona Elisabeth Pedersen¹ | ¹Nofima AS, Ås, Norway.

Current estimates predict that global food production needs to increase and adapt in response to population growth and dietary changes. Better utilization of animal and plantbased side-streams from food production will be necessary to achieve a circular economy while maintaining the nutritional needs of the global population. Muscle health and regenerative properties are important during aging, diabetes, and normal skeletal muscle homeostasis, and are highly affected by nutrient intake. Muscle stem cells, also known as satellite cells, play a key role in muscle repair and regeneration. Metabolic pathways involving glucose utilization are essential for the proper functioning of these stem cells. Muscle stem cells and their regenerative properties have been shown to be stimulated by small peptides and chemical compounds like phytochemicals, influencing proliferation, glucose uptake, and protein synthesis. In this study, we explore the potential of hydrolysates from side-streams of the chicken industry and phytochemical-rich extracts from plant sidestreams in glucose uptake and muscle regeneration. Our data showed that peptides generated from chicken hydrolysates and extracts from cauliflower, kale, and apple stimulated the proliferation of bovine muscle cells using the Incucyte live cell system. Further analysis revealed that chicken hydrolysate increased glucose uptake and protein synthesis in both bovine and rat muscle cells through regulation of the MEK and ERK signaling pathways. In conclusion, we observe bioactive compounds in rest-raw materials from both plants and chicken that can be promising nutraceutical supplements for muscle health.