Diabetes mellitus and prostate cancer metabolism: Is there a relationship?
Submitted: July 20, 2018
Accepted: July 27, 2018
Published: September 30, 2018
Accepted: July 27, 2018
Abstract Views: 2147
PDF: 852
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Authors
Urology and Transplantation Department, Coimbra Hospital and University Centre, Coimbra, Portugal.
CNC.IBILI, University of Coimbra, Coimbra; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty of Medicine University of Coimbra, Portugal.
Urology and Transplantation Department, Coimbra Hospital and University Centre, Coimbra, Portugal.
Urology and Transplantation Department, Coimbra Hospital and University Centre, Coimbra, Portugal.
CNC.IBILI, University of Coimbra, Coimbra; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty of Medicine University of Coimbra, Portugal.
CNC.IBILI, University of Coimbra, Coimbra; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra; CICS-UBI, Health Sciences Research Centre, University of Beira Interior, Covilhã; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty of Medicine University of Coimbra, Portugal.
CNC.IBILI, University of Coimbra, Coimbra; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty of Medicine University of Coimbra, Portugal.
Institute of Biophysics, Faculty of Medicine, University of Coimbra; Pathology Department, Coimbra Hospital and University Centre, Coimbra, Portugal.
Urology and Transplantation Department, Coimbra Hospital and University Centre, Coimbra; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty of Medicine University of Coimbra, Portugal.
Urology and Transplantation Department, Coimbra Hospital and University Centre, Coimbra; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty
of Medicine University of Coimbra, Portugal.
CNC.IBILI, University of Coimbra, Coimbra; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty of Medicine University of Coimbra, Portugal.
Urology and Transplantation Department, Coimbra Hospital and University Centre, Coimbra; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty
of Medicine University of Coimbra, Portugal.
CNC.IBILI, University of Coimbra, Coimbra; Institute of Biophysics, Faculty of Medicine, University of Coimbra, Coimbra; Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO) Faculty of Medicine University of Coimbra, Portugal.
Materials and methods: Two PCa cell lines (LNCap and PC3) were cultured in RPMI medium with different glucose concentrations [5mM (LG) and 25mM (HG)]. Expressions of androgen receptor, Her2/neu and glucose transporters (GLUT1, 3, 5 and 12) were evaluated by flow cytometry. Proliferation rate was assessed by colorimetric assay MTT and cellular characterization was performed by haematoxylin and eosin staining. Additionally, we performed a cross sectional analysis of 704 patients undergoing radical prostatectomy who were divided into two groups (diabetic and non-diabetic). An analysis of clinical and histological data seeking to identify the differences on tumor aggressiveness between the two groups was performed.
Results: In LNCaP cell line, when the glucose concentration in the medium increased, there was an increased in AR expression. Regarding expression of Her2/neu receptor, medium’s glucose concentration significantly changed the expression of this receptor in both PC3 and LNCaP cell lines. Growth rate was higher on the HG medium for both cell lines. The clinical study of patients undergoing radical prostatectomy revealed no relationship between the presence of diabetes and the development of more aggressive tumours. Diabetic patients had significantly higher prostatic volumes, however, no significant difference was found between the relapse risk classification or the ISUP classification between the two groups.
Conclusions: Our results showed that medium glucose concentration could influence prostate cancer cells growing but not the aggressiveness.
How to Cite
Antunes, H. P., Teixo, R., Carvalho, J. A., Eliseu, M., Marques, I., Mamede, A., Neves, R., Oliveira, R., Tavares-da-Silva, E., Parada, B., Abrantes, A. M., Figueiredo, A., & Botelho, M. F. (2018). Diabetes mellitus and prostate cancer metabolism: Is there a relationship?. Archivio Italiano Di Urologia E Andrologia, 90(3), 184–190. https://doi.org/10.4081/aiua.2018.3.184
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