The effect of vitamin D in vitro supplementation on sperm deoxyribonucleic acid fragmentation

Submitted: August 2, 2024
Accepted: August 26, 2024
Published: October 29, 2024
Abstract Views: 186
PDF: 42
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Objective: This study aimed to identify the direct effect of vitamin D on sperm DNA integrity after swim-up preparation.
Materials and methods: Normozoospermia samples were gathered from 12 men and assessed for their baseline characteristics, including DNA Fragmentation Index (DFI). Each sample was then prepared using the swim-up method. Half of the samples were incubated with vitamin D, while the other half were incubated with a standard sperm-washing medium.
Results: Vitamin D significantly reduced the DFI compared to the baseline (5.5 ± 3.4% versus 17.6 ± 4.2%; p<0.001) and the swim-up-only group (5.5 ± 3.4% versus 12.0 ± 4.2%; p< .001). Microscopic examination reflected these results, showing a reduction in the number of small halos and no halos with an increased appearance of large to medium-sized halos.
Conclusions: These results suggest that vitamin D incubation is valuable in protecting sperm from DNA damage that develops during sperm preparation. However, additional investigation is warranted to explore other preparation methods and to elucidate the underlying mechanisms.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Agarwal A, Baskaran S, Parekh N, et al. Male infertility. Lancet 2021; 397:319-33. DOI: https://doi.org/10.1016/S0140-6736(20)32667-2
Turner KA, Rambhatla A, Schon S, et al. Male Infertility is a Women’s Health Issue-Research and Clinical Evaluation of Male Infertility Is Needed. Cells 2020; 9:990. DOI: https://doi.org/10.3390/cells9040990
World Health Organization. 2020. Infertility. accessed May 2024. Available at: https://www.who.int/news-room/fact-sheets/detail/infertility
Asadi A, Ghahremani R, Abdolmaleki A, Rajaei F. Role of sperm apoptosis and oxidative stress in male infertility: A narrative review. Int J Reprod Biomed 2021; 19:493-504. DOI: https://doi.org/10.18502/ijrm.v19i6.9371
Agarwal A, Selvam MK, Baskaran S, Cho CL. Sperm DNA damage and its impact on male reproductive health: a critical review for clinicians, reproductive professionals and researchers. Expert Rev Mol Diagn 2019; 19:443-57. DOI: https://doi.org/10.1080/14737159.2019.1614916
Ribas-Maynou J, Yeste M. Oxidative Stress in Male Infertility: Causes, Effects in Assisted Reproductive Techniques, and Protective Support of Antioxidants. Biology (Basel) 2020; 9:77. DOI: https://doi.org/10.3390/biology9040077
Gualtieri R, Kalthur G, Barbato V, et al. Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development. Antioxidants (Basel) 2021; 10.1025 DOI: https://doi.org/10.3390/antiox10071025
Dutta S, Majzoub A, Agarwal A. Oxidative stress and sperm function: A systematic review on evaluation and management. Arab J Urol 2019; 17:87-97. DOI: https://doi.org/10.1080/2090598X.2019.1599624
Marzano G, Chiriacò MS, Primiceri E, et al. Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities. Biotechnol Adv 2020; 40:107498. DOI: https://doi.org/10.1016/j.biotechadv.2019.107498
Aitken RJ, Drevet JR. The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword. Antioxidants 2020; 9:111. DOI: https://doi.org/10.3390/antiox9020111
Natali, I. Sperm Preparation Techniques for Artificial Insemination - Comparison of Sperm Washing, Swim Up, and Density Gradient Centrifugation Methods. In: Manafi, M, eds. Artificial Insemination in Farm Animals. IntechOpen; 2011; Available from: https://www.intechopen.com/chapters/16102 DOI: https://doi.org/10.5772/17026
Wildy ML, Boyd L, Fourie J, et al. The Development of a Simplified Swim-up Method for Sperm Processing. Journal of Infertility and Reproductive Biology 2021; 9:160-7.
World Health Organization. WHO laboratory manual for the examination and processing of human semen. Geneva: World Health Organization 2021; 6:1-276.
Ozkavukcu S, Hughes G, Barratt CL. Sperm Preparation Techniques and Advanced Sperm Selection for Intracytoplasmic Sperm Injection. In: Textbook of Assisted Reproductive Techniques: Volume 1: Laboratory Perspectives, Sixth Edition. 2023; 1:58-69. DOI: https://doi.org/10.1201/9781003268598-7
Xue X, Wang WS, Shi JZ, et al. Efficacy of swim-up versus density gradient centrifugation in improving sperm deformity rate and DNA fragmentation index in semen samples from teratozoospermic patients. J Assist Reprod Genet 2014; 31:1161-6. DOI: https://doi.org/10.1007/s10815-014-0287-z
Raad G, Bakos HW, Bazzi M, et al. Differential impact of four sperm preparation techniques on sperm motility, morphology, DNA fragmentation, acrosome status, oxidative stress, and mitochondrial activity: A prospective study. Andrology 2021; 9:1549-59. DOI: https://doi.org/10.1111/andr.13038
Beygi Z, Forouhari S, Mahmoudi E, et al. Role of Oxidative Stress and Antioxidant Supplementation in Male Fertility. Curr Mol Med 2021; 21:265-82. DOI: https://doi.org/10.2174/1566524020999200831123553
Güngör K, Güngör ND, Başar MM, et al. Relationship between serum vitamin D levels semen parameters and sperm DNA damage in men with unexplained infertility. Eur Rev Med Pharmacol Sci 2022; 26:499-505.
Jensen MB, Bjerrum PJ, Jessen TE, et al. Vitamin D is positively associated with sperm motility and increases intracellular calcium in human spermatozoa. Hum Reprod 2011; 26:1307-17. DOI: https://doi.org/10.1093/humrep/der059
Wiseman H. Vitamin D is a membrane antioxidant. Ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS Lett 1993; 326:285-8. DOI: https://doi.org/10.1016/0014-5793(93)81809-E
Zmijewski MA. Nongenomic Activities of Vitamin D. Nutrients 2022; 14:5104. DOI: https://doi.org/10.3390/nu14235104
Hanel A, Carlberg C. Vitamin D and evolution: Pharmacologic implications. Biochem Pharmacol 2020; 173:113595. DOI: https://doi.org/10.1016/j.bcp.2019.07.024
Gasperini B, Falvino A, Piccirilli E, et al. Methylation of the Vitamin D Receptor Gene in Human Disorders. Int J Mol Sci 2024;25:107. DOI: https://doi.org/10.3390/ijms25010107
Yang H, Li G, Jin H, et al. The effect of sperm DNA fragmentation index on assisted reproductive technology outcomes and its relationship with semen parameters and lifestyle. Transl Androl Urol 2019; 8:356. DOI: https://doi.org/10.21037/tau.2019.06.22
Blaseg E, Wald TV, Hansen KA. Vitamin D levels and human sperm DNA fragmentation: a prospective, cohort study. Basic Clin Androl 2022; 32:14. DOI: https://doi.org/10.1186/s12610-022-00166-8
Banks N, Sun F, Krawetz SA, et al. Male vitamin D status and male factor infertility. Fertil Steril 2021; 116:973-9. DOI: https://doi.org/10.1016/j.fertnstert.2021.06.035
Maghsoumi-Norouzabad L, Zare Javid A, Mansoori A, et al. Evaluation of the effect of vitamin D supplementation on spermatogram, seminal and serum levels of oxidative stress indices in asthenospermia infertile men: a study protocol for a triple-blind, randomized controlled trial. Nutrition Journal 2021; 20:1-11 DOI: https://doi.org/10.1186/s12937-021-00711-7
Zeqiraj A, Beadini S, Beadini N, et al. Sperm DNA Fragmentation, Determined Using the Sperm Chromatin Dispersion (SCD) Test, A Study in Republic of Kosovo Population. Int J Biol 2018; 10:14-8. DOI: https://doi.org/10.5539/ijb.v10n3p14
Cito G, Cocci A, Micelli E, et al. Vitamin D and Male Fertility: An Updated Review. World J Mens Health 2020; 38:164-77. DOI: https://doi.org/10.5534/wjmh.190057
Adamczewska D, Słowikowska-Hilczer J, Walczak-Jedrzejowska R. The Association between Vitamin D and the Components of Male Fertility: A Systematic Review. Biomedicines 2022; 11:90. DOI: https://doi.org/10.3390/biomedicines11010090
Amorini AM, Listorti I, Bilotta G, et al. Antioxidant-Based Therapies in Male Infertility: Do We Have Sufficient Evidence Supporting Their Effectiveness? Antioxidants 2021; 10:220. DOI: https://doi.org/10.3390/antiox10020220
Babakhanzadeh E, Nazari M, Ghasemifar S, Khodadadian A. Some of the Factors Involved in Male Infertility: A Prospective Review. Int J Gen Med 2020; 13:29-41. DOI: https://doi.org/10.2147/IJGM.S241099
Halim B, Girsang E, Nasution SLR, Manalu P. Hambatan Akses Pelayanan Infertilitas pada Pasien dari Kawasan Urban dan Rural yang Berobat di Klinik Bayi Tabung Halim Fertility Center RSIA Stella Maris. Media Kesehatan Masyarakat Indonesia 2020; 19:272-8. DOI: https://doi.org/10.14710/mkmi.19.4.272-278
Pakpahan C, Rezano A, Margiana R, et al. The Association Between Lipid Serum and Semen Parameters: a Systematic Review. Reprod Sci 2023; 30:761-71. DOI: https://doi.org/10.1007/s43032-022-01040-8
Karimi E, Heshmati J, Shirzad N, et al. The effect of synbiotics supplementation on anthropometric indicators and lipid profiles in women with polycystic ovary syndrome: a randomized controlled trial. Lipids Health Dis 2020; 19:60. DOI: https://doi.org/10.1186/s12944-020-01244-4
Barbarosie C, Agarwal A, Henkel R. Diagnostic value of advanced semen analysis in evaluation of male infertility. Andrologia 2021; 53:e13625. DOI: https://doi.org/10.1111/and.13625
Ayad BM, Oyeyipo IP, Van der Horst G, Du Plessis SS. Cementing the relationship between conventional and advanced semen parameters. Middle East Fertil Soc J 2021; 26:1-10. DOI: https://doi.org/10.1186/s43043-021-00086-z
Aitken RJ. Impact of oxidative stress on male and female germ cells: implications for fertility. Reproduction 2020;159:R189-201 DOI: https://doi.org/10.1530/REP-19-0452
Halim B, Angellee J, Lubis HP, Bachsinar B. Sperm Quality and Deoxyribonucleic Acid Fragmentation after 5 and 10 min Centrifugation with Swim-Up Processing Technique: A Prospective Cohort Study. Open Access Maced J Med Sci. 2021; 9:626-30. DOI: https://doi.org/10.3889/oamjms.2021.6458
Agarwal A, Farkouh A, Saleh R, et al. Technical Aspects and Clinical Limitations of Sperm DNA Fragmentation Testing in Male Infertility: A Global Survey, Current Guidelines, and Expert Recommendations. World J Mens Health 2024; 42:202-15. DOI: https://doi.org/10.5534/wjmh.230076
Moghadam MT, Fard YA, Saki G, Nikbakht R. Effect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation. Iran J Basic Med Sci 2019; 22:1036.
Moghadam MT, Hamidian O, Mansouri E, Nikbakht R. Effects of vitamin D3 on the level of heat shock protein 70 and oxidative stress in human sperm: a pilot study. Middle East Fertil Soc J 2020;25:1-8. DOI: https://doi.org/10.1186/s43043-020-00036-1
Andri Rezano, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, West Java

Andrology Study Program, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia

Tjahjo Djojo Tanojo, Andrology Study Program, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java

Andrology Clinic, Dr. Soetomo Academic Hospital, Surabaya, East Java, Indonesia

Maria P.B.D. Pramesti, Andrology Study Program, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java

Department of Biomedical Sciences, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia

How to Cite

Rezano, A., Rezkiawan, D., Lie, V., Srisadono, A., Rivaldo, R. M., Purba, A. R., Adha, M. J., Tanojo, T. D., & Pramesti, M. P. (2024). The effect of vitamin D in vitro supplementation on sperm deoxyribonucleic acid fragmentation. Archivio Italiano Di Urologia E Andrologia. https://doi.org/10.4081/aiua.2024.12891