Prevalence, characterization, antimicrobial susceptibility pattern and factors associated with group B streptococci (Streptococcus agalactiae) from clinical and non- clinical sources in South-East Nigeria
https://doi.org/10.4081/jbr.2022.9922
Accepted: May 9, 2022
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Many years ago most human infections caused by aerobic streptococci, were attributed to Lancefield groups A and D or to the so called non-groupable viridans streptococci. The first human isolates of the Group B Streptococci (GBS) were isolated from the vagina of post-partum women. This study is aimed at assessing GBS colonization of some clinical and common non-clinical surfaces. This study utilized a total of 615 samples (300 clinicals and 315 non-clinicals). Samples were from neonates and women who were respectively seen at the Institute of Child Health, of the University of Nigeria Teaching Hospital and Amblim Reference Laboratory in Enugu. The 300 clinical samples were cultured from 119 pregnant and 79 non-pregnant women, 52 neonates and 25 (7 primary and 18 secondary infertility) infertile couples (50 individuals). All samples were cultured on a modified Islam medium and identification carried out using standard bacteriological methods. Characterizations of the GBS isolates were carried out using sodium hippurate reactions, aesculin reactions, Christie, Atkin Muchin–Patterson (CAMP) test and definite hydrolysis on blood agar. The overall GBS isolation of 15.8% was observed in the clinical and non-clinical surfaces assessed. For the clinical subjects, GBS colonization of 11.9%, 7.6%, 9.6% and 17.0% were observed in the pregnant women, non-pregnant women, neonates and couples admitted for infertility. Prevalence of GBS isolation on inflamed cattle udder and milk products were 4.0% and 83.3%, respectively. Gestational period was associated with GBS colonization in pregnant women. All the GBS isolates were susceptible to penicillin and methicillin while all were resistant to streptomycin, tetracycline and sulphafurazole. GBS colonization of vagina was found and this poses a risk for neonatal sepsis.
Lancefield RC, Hare SJ. The serological differentiation of pathogenic and non-pathogenic strains of hemolytic streptococci from parturient women. J Exp Med. 1935; 61(3):335-49.
Fry RM. Fatal Infections by haemolytic Streptococcus group B. Lancet 1938;1:199-201.
Facklam RR, Smith PB. The gram positive cocci. Hum Pathol 1976;7:18.
Mu R, Kim BJ, Paco C, et al. Identification of a group B streptococcal fibronectin binding protein, SfbA, that contributes to invasion of brain endothelium and development of meningitis. Infect Immun 2014;82:2276-86.
Christie R, Atkins NE, Munch- Peterson E. A note on a lytic Phenomenon shown by group B streptococci. Aust J Exp Biol Med Sci 1944;23:197-200.
Khalil MR, Uldbjerg N, Thorsen PB, Møller JK. Intrapartum PCR assay versus antepartum culture for assessment of vaginal carriage of group B streptococci in a Danish cohort at birth. PLoS One 2017;12:e0180262.
Cools P, Jespers V, Hardy L, et al. A multi-country cross-sectional study of vaginal carriage of group B streptococci (GBS) and Escherichia coli in resource-poor settings: prevalences and risk factors. PloS One 2016;11:e0148052.
Anthony BF, Okada DM. The emergence of group B streptococci infections of the new born infant. Annu Rev Med 1977;28:355-69.
Lippincott WA, Strohl H, Rouse-Bruce DF. General classification of streptococci. Lippincott’s illustrated review microbiology. 2001; p.146.
Kosheleva NG, Zatsiorskaia SL. GBS, a bacterium that may cause devastating disease. Akush Gineko (Mosk) 1994;6:31-3.
Hoshina K, Kadoi N, Nishida H, Kaneko K, Matsuda S. Group B streptococcal infections in neonates. Nippon Sanka Fujinka Gakkai Zasshi 1994;46:497-502.
Baker C. 2000 Group B Streptococcal infections. In Streptococcal infections. D. Stevens. And Kaplan e (eds). New York: Oxford University Press. 2000; 222-37
Hickman ME, Rench MA, Ferrieri P, Baker CJ. Changing epidemiology of group B streptococcal colonization. Paediatric 2001;104:203-9.
Schrag SJ, Zell ER, Lynfield R. A population-based comparison of strategies to prevent early-onset group B streptococcal disease in neonates. NEJM 2002;347:233.
Cheesbrough, M. District Laboratory Practice in Tropical Countries, Part 2; Cambridge University Press. 1999.
Collins CH, Lyne PM. Microbial methods, Tenth edition. Arnold, Hodder Headline Group, London, 1999.
Merriit K, Jacobs NJ. Characterization and Incidence of Pigment Production by
Human Clinical Group B Streptococci. Journal of Clinical Microbiology. 1978;8(1); 105-7.
Wessel MR, Kasper DL. Group B Streptococcus In: Infectious disease. Philadelphia. 1997; p. 216.
Edwards MS, Nizet V, Baker CJ. Group B Streptococcal Infections. 6th ed. In: infectious diseases of the fetus and newborn infant, Phiiladelphia, 2006; p.403.
Onipede A, Adefusi O, Adeyemi A, et al. Group B streptococcus carriage during late pregnancy in Ile-Ife, Nigeria. Afr J Chin Exp Microbiol 2012;13:135-43.
Akinniyi Am, Adesiyun AG, Kolawale A, et al. The prevalence of asymptomatic group B streptococcal infection and antimalarial sensitivity pattern among parturients at Ahmadu Bello University Teaching Hospital, Zaria, Nigeria. Trop Obstet Gynaecol 2017;34:182-7.
Nwachukwu N, Utsalo SJ, Kanu I, Anyanwu E. Genital colonization of Group B streptococcus at term pregnancy in Calabar, Nigeria. J Pediatr Neonatol 2007:7:1-4.
Lu B, Li D, Sui W, Huang L, Lu X. Epidemiology of group B streptococcus isolated from pregnant women in Beijing, China. Chin Microbiol Infect 2014;20:370-3.
Woldu ZL, Teklehaimanot TG, Waji ST, Gebremanriam MY. The prevalence of Group B streptococcus rect-vaginal colonization and antimicrobial susceptibility pattern in pregnant mothers at two hospitals in Addis-Ababa, Ethiopia. Reproductive Health 2014;11:80.
Bolukaoto JY, Monyama CM, Chukwu MO, et al. Antibiotic resistance of Streptococcus agalactiae isolated from pregnant women in Garankuwa, South Africa. BMC Res Notes 2015;8:364.
Boyer KM, Gadazalla CA, Burd LI, et al. Selective intrapartum chemoprophylaxis of neonatal group B streptococcal early onset disease. I. Epidermiologic rationale. Infect Dis 1983;148:795-801.
Jisuvei SC, Osoti A, Njeri MA. Prevalence antimicrobial susceptibility patterns, serotypes and risk factors for group B streptococcus rectovaginal isolates among pregnant women at Kentalta National Hospital, Kenya; a cross-sectional study. BMC Infect Dis 2020;20:302.
Donbraye-Emmanuel OO, Okonko ID, Donbraye E, et al. Isolation and characterization of Group B streptococci and other pathogens among pregnant women in Ibadan Southwest Nigeria. J Appl Biosci 2010;29:1781-92.
Dzowela T, Komolafe OO, Igbigbi A. Prevalence of group B streptococcus colonization in antenatal women at Queen Elizabeth central Hospital, Blantyre – A preliminary Study. Malawi Med J 2005;17:97-9.
Kardos S, Tothpal A, Laub K, et al. High prevalence of group B streptococcus ST17 hypervirulente clone among non-pregnant patients from Hungarian Venerology clinic BCM Infect Dis 2019;19:1009.
Moraleda C, Benmessaoud R, Esteban J, et al. Prevalence, antimicrobial resistance and serotype distribution of group B streptococcus isolated among pregnant women and newborns in Rabat, Morocco. J Med Microbiol 2018;67:652-61.
Santhanam S, Jose R, Sahni RD, et al. Prevalence of group B streptococcal colonization among pregnant women and neonates in tertiary hospital India. J Turk Ger Gynecol Assoc 2017;18:181-4.
Huang J, Lin X, Zhu Y, Chen C. Epidemiology of Group B Streptotoccal infection in pregnant women and diseased infants in mainland China. Pediatrics Neonatol 2019;60:487-95.
Roberts DJ. Perinatal infection. In: Kradin RL (ed), Diagnostic pathology of infectious disease (2nd ed). Elsevier; 2018.
CDC. Prevention of perinatal group B streptococcal disease: a public health perspective. MMWR 1996;45:1-24.
Morinis J, Shah J, Murthy P, Fulford RL (ed), Diagnostic pathology of infectious disease (2nd ed). Elsevier; 2018.
Ross PW, Cumming CG: Group B Streptococci in women attending a sexually transmitted disease clinic. J Inf Secur 1982;4:161-6.
Manning SD, Neighbors K, Tallman PA, et al. Prevalence of group B streptococcus colonization and potential for transmission by casual contact in healthy young men and women. Clin Infect Dis 2004;39:380-8.
Ruggeri M, Cannas S, Cubeddu M, et al. Bacterial agents as cause of infertility in humans. New Microbiologica 2016;39:206-9.
Botelho CAN, Ferreiro AFM, Francalanzza SEL, et al. A perspective on the potential zoonotic role of Streptococcus agalactiae: Searching for a missing link in alternative transmission routes. Front Microbiol 2018;9:608.
Finch LA, Martin DR. Human and bovine group B streptococci: two distinct population. J Appl Bacteriol 1984;57:273-8.
Manning SD, Springman AC, Million AD, et al. Association of group B streptococcus colonization and bovine exposure: A prospective Cross-Sectional cohort study. PloS One 2010:5:8795.
Quiroga M, Pegel E, Oviedo P, et al. Antibiotic susceptibility patterns and prevalence of group B streptococcus isolated from pregnant women in Misione, Argentina. Brazilian J Microbiol 2008;39:245-50.
Nguyen F, Starosta AL, Arenz S, et al. Tetracyclin antibiotics and resistance mechanisms. Biol Chem 2014;395:559-75.
Springer B, Kidan YG, Prammanan T, et al. Mechanism of streptomycin resistance: selection of mutation in the 16S rRNA gene conferring resistance. Antimicrob Agents Chemother 2001;45(10):2877-84.
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