Anti-inflammatory, antibacterial and antioxidant activities of the medicinal species <i>Atractylis cancellata</i>

Sara Mouffouk; Chaima Mouffouk; Soumia Mouffouk; Ahmed Hachem Mekki; Abderrahman Moumen Messaoud; Hamada Haba

doi:10.4081/jbr.2023.11096

Authors

Sara Mouffouk Laboratory of Cellular and Molecular Physio-Toxicology-Pathology and Biomolecules (LPTPCMB), Department of Biology of Organisms, Faculty of Natural and Life Sciences, University of Batna 2 Mustapha Benboulaid, Batna, Algeria.
Chaima Mouffouk Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Matter Sciences, University of Batna 1, Batna, Algeria; Department of Pharmacy, Faculty of Medicine, University of Batna 2 Mustapha Benboulaid, 05000 Batna, Algeria. https://orcid.org/0000-0003-1436-8685
Soumia Mouffouk Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Matter Sciences, University of Batna 1, Batna, Algeria.
Ahmed Hachem Mekki Central Laboratory of Medical Biology (CLMB)–Center for the Fight Against Cancer (CFAC) Batna, Algeria. https://orcid.org/0009-0000-1347-8810
Abderrahman Moumen Messaoud Central Laboratory of Medical Biology (CLMB)–Center for the Fight Against Cancer (CFAC) Batna, Algeria.
Hamada Haba
hamada.haba@univ-batna.dz
Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Matter Sciences, University of Batna 1, Batna, Algeria. https://orcid.org/0000-0002-7979-5007

This research is focused on the estimation of total bioactive contents and the evaluation of in vitro pharmacological activities of crude extracts (petroleum ether, ethyl acetate and n-butanol) obtained from the species Atractylis cancellata. The antioxidant activity was assessed by three different techniques. The antibacterial activity was determined using the agar disk diffusion assay against five bacterial strains. Furthermore, the anti-inflammatory activity was evaluated by the ovalbumin method. According to the results, A. cancellata extracts are rich in several classes of secondary metabolites, especially steroids, triterpenoids, flavonoids, and alkaloids. In addition, the tested extracts showed very interesting antioxidant activities in DPPH (2,2-Diphenyl-1-picrylhydrazyl) and FRAP assays and important correlation coefficients between the results of antioxidant activities and total phenolic and flavonoid contents were found. Moreover, all the tested extracts displayed an antibacterial effect at least against three bacterial strains. The petroleum ether extract inhibited the growth of all the tested bacteria in a dose-dependent manner except Escherichia coli ATCC 25922 and it revealed a strong anti-inflammatory activity (81.77±0.05%). We conclude that A. cancellata could be an important source of natural pharmacological candidates against oxidative stress, inflammatory and microbial diseases.

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Mouffouk, S., Mouffouk, C., Mouffouk, S., Mekki, A. H., Moumen Messaoud, A., & Haba, H. (2023). Anti-inflammatory, antibacterial and antioxidant activities of the medicinal species <i>Atractylis cancellata</i>. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 96(1). https://doi.org/10.4081/jbr.2023.11096