Antioxidant and antifungal activities in vitro of essential oils and extracts of twelve Algerian species of Thymus against some mycotoxigenic Aspergillus genera

Submitted: November 25, 2021
Accepted: December 28, 2021
Published: January 11, 2022
Abstract Views: 2269
PDF: 541
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The aim of the study was to determine the phenolic and flavonoid content of Essential Oils (EOs), chloroform and ethanolic extracts of 12 Algerian Thymus species and evaluate their antioxidant and antifungal activities. EOs (1.73±0.30– 15.00±1.24μg/mg), chloroform extracts (33.8±2.42– 160.93±3.88μg/mg) and ethanol extracts (27.01±3.56 – 148.46±4.40μg/mg) showed considerable phenolic content. Flavonoids values of chloroform extracts ranged between 3.39±0.17 and 20.27±0.29μg/mL while ethanolic extracts values ranged between 2.81±0.11 and 26.64±0.18μg/mg. Results of DPPH showed that EOs, chloroform and ethanolic extracts exhibited strong radical scavenging activity (IC50=21.75±6.54– 338.22±2.99μg/mL, 22.91±5.59–90.93±1.36μg/mL, and 33.51±5.72–103.80±4.54μg/m, respectively). Inhibition of β-carotene bleaching was potentially performed by all EOs (66.48±2.41–94.06±2.68%), chloroform extracts (68.98±1.58– 95.30±1.99%), and ethanolic extracts (62.15±2.51–92.36±1.15%). The antifungal activity of EOs and extracts was tested using the Minimum Inhibitory Concentration (MIC) and minimum fungicidal concentration (MFC). The EOs (0.1±0.00mg/mL– 1.06±0.46mg/mL), chloroform (0.1±0.00 mg/mL– 1.06±0.46mg/mL) and ethanol (0.1±0.00mg/mL–1.6±0.00 mg/mL) showed remarkable antifungal activity against mycotoxigenic Aspergillus genera. The MFC of EOs (1.0±0.34mg/mL and >4.8mg/mL), chloroform (0.26±0.11mg/mL and >1.6 mg/mL) and ethanol (0.2±0.00mg/mL and >1.6 mg/mL) were fungicidal in nature higher than MICs. The findings of the study indicated that Thymus spp. EOs and extracts could be used as natural alternatives for food industry

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Samson RA, Hong SB, Frisvad JC. Old and new concepts of species differentiation in Aspergillus. Med Mycol 2006;44:133-48. DOI: https://doi.org/10.1080/13693780600913224
Burt S. Essential oils: Their antibacterial properties and potential applications in foodsea review. Int J Food Microbiol 2004;94:223-53. DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
Saad M, Fadlia M, Bouaziz A, et al. Anticandidal activity of the essential oils of Thymus maroccanus and Thymus broussonetii and their synergism with amphotericin B and fluconazole. Phytomedicine 2010;17:1057-60. DOI: https://doi.org/10.1016/j.phymed.2010.03.020
Hazzit M, Baaliouamer A. Composition of the essential oils of the leaves and flowers of Thymus pallescens de Noé and Origanum floribundum Munby from Algeria. J Essent Oil Res 2011;3:267-70. DOI: https://doi.org/10.1080/10412905.2009.9700166
Dewanto V, Wu X, Adom KK, et al. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agr Food Chem 2002;50:3010-4. DOI: https://doi.org/10.1021/jf0115589
Pękal A, Pyrzynska K. Evaluation of aluminium complexation reaction for flavonoid content assay. Food Anal Methods 2014;7:1776-82. DOI: https://doi.org/10.1007/s12161-014-9814-x
Nikhat F, Satynarayana D, Subhramanyam EVS. Isolation, charactrisation and screening of antioxidant activity of the roots of Syzygiumcuminii (L) Skeel. Asian J Res Chem 2009;2:218-21.
Miraliakbari H, Shahidi F. Antioxidant activity of minor components of tree nut oils. Food Chem 2008;111:421-27. DOI: https://doi.org/10.1016/j.foodchem.2008.04.008
Prakash B, Singh P, Kedia A, et al. Assessment of some essential oils as food preservatives based on antifungal, antiaflatoxin, antioxidant activities and in vivo efficacy in food system. Food Res Int 2012;49:201-8. DOI: https://doi.org/10.1016/j.foodres.2012.08.020
Ismaili H, Tortora S, Sosa S, et al. Topical anti-inflammatory activity of Thymus willdenowi. J Pharm Pharmacol 2001;53:1645. DOI: https://doi.org/10.1211/0022357011778250
Ballester-Costa C, Sendra E, Fernández-López J, et al. Assessment of antioxidant and antibacterial properties on meat homogenates of essential oils obtained from four Thymus species achieved from organic growth. Foods 2017;6:59. DOI: https://doi.org/10.3390/foods6080059
Amarti F, Satrani B, Ghanmi M, et al. Composition chimique et activité antimicrobienne des huiles essentielles de Thymus algeriensis Boiss. & Reut. et Thymus ciliatus (Desf.) Benth. du Maroc. Biotechnol Agron Soc Environ 2010;14:141-8.
Jukic M, Milos M. Catalytic oxidation and antioxidant propertic of thyme essential oils (Thymus vulgarae L.). Croat Chem Acta 2005;78:105-10.
Kulšic T, Dragovic-Uzelac V, Miloš M. Antioxidant activity of aqueous tea infusions prepared from oregano, thyme and wild thyme. Food Technol Biotechnol 2006;44:485-92.
Amić D, Davidović-Amić D, Bešlo D, et al. Structure–radical scavenging activity relashionships of flavonoids. Croat Chem Acta 2003;76:55-61.
Maggi F, Bramucci M, Cecchini C, et al. Composition and biological activity of essential oil of Achillea ligustica All. (Asteraceae) naturalized in central Italy: Ideal candidate for anti-cariogenic formulations. Fitoterapia 2009;80:313-19. DOI: https://doi.org/10.1016/j.fitote.2009.04.004
Deba F, Xuan T, Yasuda M, et al. Chemical composition and antioxi-dant, antibacterial and antifungal activities of the essential oils from Bidens pilosa Linn. var. Radiata. Food Contr 2008;19:346-52. DOI: https://doi.org/10.1016/j.foodcont.2007.04.011
Sandhar HK, Kumar B, Prasher S, et al. A Review of phytochemistry and pharmacology of flavonoids. Int Pharm Sci 2011;1:25-41.
Aligiannis N, Kalpotzakis E. Composition and antimicrobial activity of the essential oils of two Origanum species. J Agr Food Chem 2001;40:4168-70. DOI: https://doi.org/10.1021/jf001494m
Stević, T, Berić T, Šavikina K, et al. Antifungal activity of selected essential oils against fungi isolated from medicinal plant. Ind Crop Prod 2014;55:116-22. DOI: https://doi.org/10.1016/j.indcrop.2014.02.011
Dikbas N, Kotan R, Dadasoglu F, et al. Control of Aspergillus flavus with essential oil and methanol extract of Satureja hortensis. Int J Food Microbiol 2008;124:179-82. DOI: https://doi.org/10.1016/j.ijfoodmicro.2008.03.034
Lahmar I, Belghith H, Ben Abdallah F, et al. Nutritional composition and phytochemical, antioxidative, and antifungal activities of Pergularia tomentosa L. Biomed Res Int 2017;2017:6903817. DOI: https://doi.org/10.1155/2017/6903817
Zaïri A, Nouir S, Zarrouk A, et al. Phytochemical profile, cytotoxic, antioxidant, and allelopathic potentials of aqueous leaf extracts of Olea europaea. Int J Food Sci 2020;8:4805-13. DOI: https://doi.org/10.1002/fsn3.1755

How to Cite

Ben Miri, Y., Taoudiat, A. ., & Mahdid, M. . (2022). Antioxidant and antifungal activities <em>in vitro</em> of essential oils and extracts of twelve Algerian species of <em>Thymus</em> against some mycotoxigenic <em>Aspergillus</em> genera. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 95(1). https://doi.org/10.4081/jbr.2022.10299