Effects of inhibition of lymphangiogenesis by the vascular endothelial growth factor receptor 3 (VEGFR-3) inhibitor, MAZ51 on full thickness wounds in mice

  • Kana Takada Department of Clinical Nursing, Graduate Course of Nursing Science, Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan.
  • Yukari Nakajima Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Tamae Urai School of Nursing, Toyama Prefectural University, Toyama, Japan.
  • Kanae Mukai Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Kimi Asano School of Nursing, Kanazawa Medical University, Uchinada, Japan.
  • Mayumi Okuwa Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
  • Junko Sugama Institute for Frontier Science Initiative, Kanazawa University, Kanazawa, Japan.
  • Toshio Nakatani | nakatosi@staff.kanazawa-u.ac.jp Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.

Abstract

We herein used MAZ51 to inhibit lymphangiogenesis and aimed to clarify the effect of inhibition of lymphangiogenesis on wound healing. BALB/c male mice were divided into two groups: the control group which was injected the dimethyl sulfoxide (DMSO), the experiment group was injected MAZ51 in the DMSO. All wounds were observed for 15 days and the wound areas were measured. Tissue samples were harvested on day 3, 7, 9, 11, 13 and 15, and subjected to immunostaining of blood vessels and lymphatic vessels. There are no significant differences between two groups in the wound area, the number of blood vessels and lymphatic vessels. The number of blood vessels peaked on day 7 in both groups as with previous studies, while the number of lymphatic vessels peaked on 13 or 15 in both groups. This result revealed delayed lymphangiogenesis in comparison with previous studies. The wound healing process in the control and experiment groups were similar, but both groups seemed delayed lymphangiogenesis comparing with previous studies. Injections of MAZ51 or/and DMSO did not affect angiogenesis, while they may affect lymphangiogenesis.

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References

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Published
2021-03-12
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Original Articles
Keywords:
Lymphangiogenesis, MAZ51, angiogenesis, VEGF-C/VEGFR-3 signaling.
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How to Cite
Takada, K., Nakajima, Y., Urai, T., Mukai, K., Asano, K., Okuwa, M., Sugama, J., & Nakatani, T. (2021). Effects of inhibition of lymphangiogenesis by the vascular endothelial growth factor receptor 3 (VEGFR-3) inhibitor, MAZ51 on full thickness wounds in mice. Veins and Lymphatics, 10(1). https://doi.org/10.4081/vl.2021.9385