Curcumin effects in inducing mRNA gene cathelidicin antimicrobial peptide in Balb/c mice infected with <em>Salmonella typhi</em>

Ami Febriza; Rosdiana Natzir; Mochammad Hatta; Cuno S.P.M Uiterwaal; Suryani As'ad; Budu; Gemini Alam; Vivien Novarina Kasim; Hasta Handayani Idrus

doi:10.4081/jbr.0.8942

https://doi.org/10.4081/jbr.0.8942

Ami Febriza | amifebriza@med.unismuh.ac.id Department of Physiology, Faculty of Medicine, University of Muhammadiyah Makassar, Makassar of Muhammadiyah Makassar, Indonesia.
Rosdiana Natzir Biochemistry Department, Indonesia.
Mochammad Hatta Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Cuno S.P.M Uiterwaal Julius Centre for Health Sciences and Primary Care, University Medical Center, Utrecht, Netherlands.
Suryani As'ad Clinical Nutritional Department, Indonesia.
Budu Department of Ophtalmology, Faculty of Medicine, Indonesia.
Gemini Alam Department of Pharmacognosy, Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia.
Vivien Novarina Kasim Faculty of Medicine, Gorontalo State University, Gorontalo, Indonesia. https://orcid.org/0000-0002-7908-3456
Hasta Handayani Idrus Department of Microbiology, Faculty of Medicine, University Muslim Indonesia, Makassar, Indonesia.

Abstract

Efforts to combine various herbal compounds are made in the wake of numerous cases of antibiotic resistance. Curcumin is an active compound found in herbal plants. It has an antimicrobial effect that can induce the expression of the mRNA Cathelidicin Antimicrobial Peptide (CAMP) gene and eradicate bacteria. Twenty-five adult BALB/c mice, aged 8–12 weeks and weighing 30–40 grams, were induced with Salmonella typhi at the intraperitoneal cavities. They were randomly allocated in equal blocks to receive CM200 (200 mg/kg of curcumin), CM400 (400 mg/kg of curcumin), CM200+vit D (200 mg/kg of curcumin with vitamin D), and positive control or negative control for 5 days. Mice were then maintained for 3 weeks to count the colonies in the post-intervention period and the level of the mRNA CAMP gene. Real-time PCR was used to measure the expression of the CAMP gene. The level of the mRNA CAMP gene expression significantly increased in CM200 (2.01±0.75) and CM400 (4.06±0.68), p_difference <0.0001. The highest increase of the CAMP gene expression was observed in CM200+vit D (5.47±0.53), p_difference <0.0001. Curcumin increased the expression of the mRNA CAMP gene.