Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

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Francesca Sardi
Martina Rossi
Sara Ferrando
Maria Angela Masini
Federico Biggi
Andrea Amaroli *
(*) Corresponding Author:
Andrea Amaroli | andrea.amaroli.71@gmail.com

Abstract

Compounds known as chemical mediators, including acetylcholine, have been found not only in humans and animals, but also in living organisms, like protozoa, which lack nervous system. In Paramecium primaurelia has been described a cholinergic system, which is proven to play an important role in cell-cell interactions during its developmental cycle. In our work we investigated the effects of exposure to simulated microgravity (3D Random Positioning Machine, 56 rpm, 10-6 g) on the cholinesterase activity of the eukaryote unicellular-organism alternative-model P. primaurelia. Our results show that the exposure of P. primaurelia to microgravity for 6 h, 24 h, 48 h affects the localization and the amount of cholinesterase activity compared to cells grown under Earth gravity conditions (1 g). However, these effects are transient since P. primaurelia restores its normal cholinesterase activity after 72 h under microgravity conditions, as well as cells exposed up to 72 h to microgravity and then placed under terrestrial gravity for 48 h.


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Author Biographies

Francesca Sardi, Laboratory of Protistology, Department of Earth Science, Environment and Life, University of Genoa


Sara Ferrando, Laboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genoa


Federico Biggi, Laboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genoa


Andrea Amaroli, Laboratory of Protistology, Department of Earth Science, Environment and Life; Department of Surgical Sciences and Integrated Diagnostic, University of Genoa