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Effects of altered gravity induced by clinorotation on the cholinesterase activity of the non-sentient model Paramecium primaurelia (Protozoa)

Francesca Sardi, Martina Rossi, Sara Ferrando, Maria Angela Masini, Federico Biggi, Andrea Amaroli
  • Francesca Sardi
    Laboratory of Protistology, Department of Earth Science, Environment and Life, University of Genoa, Italy
  • Martina Rossi
    Laboratory of Protistology, Department of Earth Science, Environment and Life, University of Genoa,
  • Sara Ferrando
    Laboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genoa, Italy
  • Maria Angela Masini
    Laboratory of Space Biology, Department of Sciences and Technology Innovation, University of Piemonte Orientale, Italy
  • Federico Biggi
    Laboratory of New Model Organism (NeMo LAB), Department of Earth, Environmental and Life Sciences, University of Genoa, Italy

Abstract

Compounds known as chemical mediators, including acetylcholine, have been found not only in humans and animals, but also in living organisms, like protozoa, which lack nervous system. In Paramecium primaurelia has been described a cholinergic system, which is proven to play an important role in cell-cell interactions during its developmental cycle. In our work we investigated the effects of exposure to simulated microgravity (3D Random Positioning Machine, 56 rpm, 10-6 g) on the cholinesterase activity of the eukaryote unicellular-organism alternative-model P. primaurelia. Our results show that the exposure of P. primaurelia to microgravity for 6 h, 24 h, 48 h affects the localization and the amount of cholinesterase activity compared to cells grown under Earth gravity conditions (1 g). However, these effects are transient since P. primaurelia restores its normal cholinesterase activity after 72 h under microgravity conditions, as well as cells exposed up to 72 h to microgravity and then placed under terrestrial gravity for 48 h.

Keywords

Microgravity; Simulated microgravity; Acetylcholinesterase; Neurotransmitters; Cholinergic system.

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Submitted: 2017-08-30 12:41:52
Published: 2018-05-23 16:53:52
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Copyright (c) 2018 Francesca Sardi, Martina Rossi, Sara Ferrando, Maria Angela Masini, Federico Biggi, Andrea Amaroli

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