Development of nanostructured bioplastic material for wound healing

Submitted: 6 October 2020
Accepted: 11 November 2020
Published: 5 February 2021
Abstract Views: 2015
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The development of new biomaterials whose characteristics are as close as possible to the properties of living human tissues is one of the most promising areas of regenerative medicine. This work aimed at creating a bioplastic material based on collagen, elastin and hyaluronic acid and studying its structure and properties to assess the prospects for further use in clinical practice. Bioplastic material was obtained by mixing collagen, hyaluronic acid and elastin in predetermined proportions with distilled water. We treated the material with photochemical crosslinking to stabilize biofilm in a liquid medium and form a nanostructured scaffold. A commercial human skin fibroblast cell culture was used to assess the biomaterial cytotoxicity and biocompatibility. The visualization and studies of the biomaterial structure were performed using light and scanning electron microscopy. It has been shown that the obtained biomaterial is characterized by high resilience; it has also a high porosity. The co-culturing of the bioplastic material and human fibroblasts did not reveal any of its cytotoxic effects on cells in culture. It was shown that the biomaterial samples could maintain physical properties in the culture medium for more than 10 days, while the destruction of the matrix was observed 3–4 weeks after the beginning of incubation. Thus, the created biomaterial can be used on damaged skin areas due to its physical properties and structure. The use of the developed biomaterial provides effective conditions for good cell proliferation, which allows us to consider it as a promising wound cover for use in clinical practice.

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Citations

Frykberg RG, Banks J. Challenges in the treatment of chronic wounds. Adv Wound Care (New Rochelle) 2015;4:560-82. DOI: https://doi.org/10.1089/wound.2015.0635
XuJ,ZhengS,HuX,LiL,LiW,ParungaoR, Wang Y, Nie Y, Liu T, Song K. Advances in the research of bioinks based on natural collagen, polysaccharide and their derivatives for skin 3d bioprinting. Polymers (Basel) 2020 May 29;12(6):1237. DOI: https://doi.org/10.3390/polym12061237
Kotenko K, Eremin I, Moroz B, Bushmanov AJu, Nadezhina NM, Galstjan IA; Grinakovskaja OS, Aksenenko AV, Deshevoj JuB, Lebedev VG, Slobodina TS, Zhgutov JuA, Lauk-Dubickij SE. Eremin PS. Cell technologies in the treatment of radiation burns: Experience burnasyan federal medical biophysical centre. Cellular Transplantation & Tissue Engineering 2012;7:97- 102.
Tottoli EM, Dorati R, Genta I, Chiesa E, Pisani S, Conti B. Skin wound healing process and new emerging technologies for skin wound care and regeneration. Pharmaceutics 2020;12. DOI: https://doi.org/10.3390/pharmaceutics12080735
Sheikh Z, Najeeb S, Khurshid Z, Verma V, Rashid H, Glogauer M. Biodegradable materials for bone repair and tissue engineering applications. Materials (Basel) 2015;8:5744-94. DOI: https://doi.org/10.3390/ma8095273
Shtil’man MI, Mezhuev YO. Implanted and unimplanted medical and biological polymers. Fibre Chemistry (English Translation of Khimicheskie Volokna) 2016;48:249-52. DOI: https://doi.org/10.1007/s10692-016-9778-2
Guan J, Sacks MS, Beckman EJ, Wagner WR. Synthesis, characterization, and cytocompatibility of elastomeric, biodegradable poly(ester- urethane)ureas based on poly(caprolactone) and putrescine. J Biomed Mater Res 2002;61:493-503. DOI: https://doi.org/10.1002/jbm.10204
Yu Z, Lili J, Tiezheng Z, Li S, Jianzhuang W, Haichao D, Kedong S, Tianqing L. Development of decellularized meniscus extracellular matrix and gelatin/chitosan scaffolds for meniscus tissue engineering. Biomed Mater Eng 2019;30:125-32. DOI: https://doi.org/10.3233/BME-191038
Reis RL, Neves N, Mano JF, Gomes ME, Marques Ap. Natural-based polymers for biomedical applications. Natural-Based Polymers For Biomedical Applications. Woodhead Publishing Series in Biomaterials. 2008:XXIII-XXV.
Azimi B, Maleki H, Zavagna L, De la Ossa JG, Linari S, Lazzeri A, Danti S. Bio-based electrospun fibers for wound healing. J Funct Biomater 2020;11. DOI: https://doi.org/10.3390/jfb11030067
Song R, Murphy M, Li C, Ting K, Soo C, Zheng Z. Current development of biodegradable polymeric materials for biomedical applications. Drug Des Devel Ther 2018;12:3117-45. DOI: https://doi.org/10.2147/DDDT.S165440
Mao AS, Mooney DJ. Regenerative medicine: Current therapies and future directions. Proc Natl Acad Sci U S A. 2015;112:14452-9. DOI: https://doi.org/10.1073/pnas.1508520112
Carruthers CA, Dearth CL, Reing JE, Kramer CR, Gagne DH, Crapo PM, Garcia O Jr, Badhwar A, Scott JR, Badylak SF. Histologic characterization of acellular dermal matrices in a porcine model of tissue expander breast reconstruction. Tissue Eng Part A 2015;21:35-44. DOI: https://doi.org/10.1089/ten.tea.2014.0095
Haugland RP, Gregory J, Spence MTZ, Johnson I D. Handbook of fluorescent probes and research products: Molecular Probes. Pennsylvania State University. 2002.
Chantre CO, Gonzalez GM, Ahn S, Cera L, Campbell PH, Hoerstrup SP, Parker KK. ACS Appl Mater Interfaces. 2019 Dec 11;11(49):45498- 45510. DOI: https://doi.org/10.1021/acsami.9b17322
Uludag H, Pandit A, Kuhn L. Editorial: Enabling biomaterials for new biomedical technologies and clcinical therapies. Front Bioeng Biotechnol 2020 Jun 5;8:559. DOI: https://doi.org/10.3389/fbioe.2020.00559
Tetsuka H, Shin SR. Materials and technical innovations in 3d printing in biomedical applications. J Mater Chem B 2020 Apr 21;8:2930- 2950. DOI: https://doi.org/10.1039/D0TB00034E
Chan WW, Yeo DCL, Tan V, Singh S, Choudhury D, Naing MW. Additive biomanufacturing with collagen inks. Bioengineering (Basel) 2020 Jul 1;7:66. DOI: https://doi.org/10.3390/bioengineering7030066

How to Cite

Gilmutdinova, I. R., Kostromina, E., Yakupova, R. D., & Eremin, P. S. (2021). Development of nanostructured bioplastic material for wound healing. European Journal of Translational Myology, 31(1). https://doi.org/10.4081/ejtm.2021.9388