The effect of Vigna unguiculata on the estrogen receptor-α expression and the endometrial thickness in rats treated with depot medroxyprogesterone acetate (DMPA)
https://doi.org/10.4081/hls.2023.11766
Accepted: 18 October 2023
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Depot medroxyprogesterone acetate (DMPA) is a contraceptive method that prevents ovulation and reduces endometrial thickness. This study aimed to investigate the influence of Vigna unguiculata (VU) on estrogen receptor-α expression and endometrial thickness in rats subjected to DMPA treatment. The research utilized a true experimental design involving 25 female Wistar rats divided into 5 experimental groups. The DMPA+VU experiment lasted for 4 weeks, and data were analyzed using a Complete Randomized Design. Estrogen receptor-α expression in the endometrium was assessed through immunohistochemical techniques, and endometrial thickness was determined via Hematoxylin-eosin (HE) staining, observed under dot slide microscopes (Olympus XC10) at 400× magnification. The study found that estrogen receptor-α expression and endometrial thickness were significantly higher in both the DMPA+VU2 and DMPA+VU3 groups compared to the DMPA group and the DMPA+VU1 group (p<0.05). DMPA treatment reduced estrogen receptor-α expression and endometrial thickness. However, the administration of Vigna unguiculata extracts at 2.5 mg/kg and 5 mg/kg led to an increase in estrogen receptor-α expression and endometrial thickness. The study implies that VU extract may have a positive impact on estrogen receptor-α expression and endometrial thickness in rats treated with depot medroxyprogesterone acetate.
Novika RGH, Hutomo CS, Wahidah NJ, et al. The Effect of Apium graveolens L. in Progesterone-Induced Blocking Factor (PIBF) During Pregnancy. Res J Pharm Technol 2022;15:4463–4468. DOI: https://doi.org/10.52711/0974-360X.2022.00748
Putri KY, Srianto P, Lestari TD, et al. Reproductive efficiency and serum progesterone concentration on dairy cattle based on blood urea nitrogen (Bun) concentrations. Iraqi J Vet Sci 2018;32:143–148. DOI: https://doi.org/10.33899/ijvs.2019.153840
Bakry S, Merhi ZO, Scalise TJ, et al. Depot-medroxyprogesterone acetate: an update. Arch Gynecol Obstet 2008;278:1-12. DOI: https://doi.org/10.1007/s00404-007-0497-z
Elfrida RY, Sari GM, Dwiningsih SR, et al. The Comparison of Total Cholesterol Level in DMPA and Combination Injection Contraception Users. Pediomaternal Nurs J 2020;6:117–124. DOI: https://doi.org/10.20473/pmnj.v6i2.20352
Batlajery J, Hamidah H, Mardiana M. Penggunaan Metode Kontrasepsi Suntikan Dmpa Berhubungan Dengan Disfungsi Seksual Wanita Pada Akseptor Kb Suntik. J Ilmu dan Teknol Kesehat 2015;2:49–56.
Mahyudanil M, Bajamal AH, Sembiring RJ, et al. The effect of progesterone therapy in severe traumatic brain injury patients on serum levels of s-100β, interleukin 6, and aquaporin-4. Open Access Maced J Med Sci 2020;8:236–244. DOI: https://doi.org/10.3889/oamjms.2020.3974
Yulianto MR, Turchan A, Notobroto HB. The effect of estrogen, progesterone, and its combination on the expression of brain-derived neurotrophic factor medula spinalis in regeneration process of peripheral nerve. Indian J Forensic Med Toxicol 2020;14:3150–3155.
Simbar M, Tehrani FR, Hashemi Z, et al. A comparative study of Cyclofem® and depot medroxyprogesterone acetate (DMPA) effects on endometrial vasculature. J Fam Plan Reprod Heal Care 2007;33:271–276. DOI: https://doi.org/10.1783/147118907782101887
Fitrianingtyas R, Christiana I. Effect of Depo Medroxsi Progesteron Acetat (DMPA) on Indeks Apoptosis Ovarium in The Exposed Rated Endometrium (Rattus Novergicus). J Kesehat dr Soebandi 2022;10:98–105. DOI: https://doi.org/10.36858/jkds.v10i2.384
Wahdah R, Ellya H, Hairina H. Respon Viabilitas Benih Kacang Tunggak Nagara (Vigna unguiculata ssp cylindrica) Akibat Pemberian Konsentrasi Ekstrak Akar Eceng Gondok (Eichhornia crassipes). Rawa Sains J Sains STIPER Amuntai 2020;10:63–73. DOI: https://doi.org/10.36589/rs.v10i2.123
Wang W, Sun Y, Liu J, et al. Soy isoflavones administered to rats from weaning until sexual maturity affect ovarian follicle development by inducing apoptosis. Food Chem Toxicol 2014;72:51–60. DOI: https://doi.org/10.1016/j.fct.2014.07.010
Veri N, Aulia F, Ratnawati R, et al. Protective effect of green tea against ovarian and endometrial apoptoses in rats treated with depot medroxyprogesterone acetate. Biomarkers Genomic Med 2015;7:105–109. DOI: https://doi.org/10.1016/j.bgm.2015.04.002
Prastiya RA, Suprayogi TW, Debora AE, et al. Green tea extract addition into a Tris-based egg yolk extender improves Bali bull sperm quality. Anim Biosci 2023;36:209–217. DOI: https://doi.org/10.5713/ab.22.0184
Susilowati S, Mustofa I, Wurlina W, et al. Green Tea Extract in the Extender Improved the Post-Thawed Semen Quality and Decreased Amino Acid Mutation of Kacang Buck Sperm. Vet Sci 2022;9 DOI: https://doi.org/10.3390/vetsci9080403
:403.
Bakry S, Aseem N MN. Cytotoxicity and genotoxicity of DMPA on female rats. Toxicol Lett 2010;196:s156–s157. DOI: https://doi.org/10.1016/j.toxlet.2010.03.539
E. Pamuji DB dan MH. Assosiation between bleeding pattern and estradiol level of depot medroxy progestreon acetat users. Ber Kedokt Masy 2008;24:51–57.
Fitrianingtyas R, Anggreni E. Pengaruh Depo Medroksi Progesteron Asetat (DMPA) Terhadap Ekspresi Estrogen Receptor-Α (Er-Α) Pada Endometrium Tikus (Rattus Novergicus) Yang Dipapar Effect of Acetic Progesteron Depo Medical (DMPA) On Expression of Receptor- α (ER-α) Estro. J Ilm Kebidanan 2019;5:3–8. DOI: https://doi.org/10.33023/jikeb.v5i1.226
Mishell Jr DR, Kltzky OA, Brenner PF, et al. The effect of contraceptive steroida on hypotalamic pituitary function. Am J Obs Gynecol 2015;128:60–74. DOI: https://doi.org/10.1016/0002-9378(77)90295-2
Moyer DL, Felix JC. The effect of progesterone and progestines on endometrial prolireratoin. Contraception 1998;57:399–403. DOI: https://doi.org/10.1016/S0010-7824(98)00047-X
Michel KG, Huijbregts RPH, Richter HE, et al. Effect of depot medroxyprogesterone acetate on human β-defensin production and structural integrity of the human vaginal epithelium. Lancet 2013;382:S25. DOI: https://doi.org/10.1016/S0140-6736(13)62273-4
Bellanti F, Matteo M, Rollo T, et al. Sex hormones modulate circulating antioxidant enzymes: impact of estrogen therapy. Redox Biol 2013;1:340-6. DOI: https://doi.org/10.1016/j.redox.2013.05.003
Darmadi D, Nurdiana, Norahmawati E. Efek Ekstrak Kacang Tunggak terhadap Osteoblas dan Osteoklas pada Tikus dengan Ovarektomi The Effect of Blackeyed Peas on Osteoblast and Osteoclast of Rat with Ovarectomy. J Kedokt Brawijaya 2011;26:151–155. DOI: https://doi.org/10.21776/ub.jkb.2011.026.03.4
Lofamia EAA, Ramos GB, Mamon MAC, et al. Isoflavone maternal-supplementation during periconception period: Influence on the reproductive organs of the first generation (F1) murine weanling-stage offspring. Asian Pacific J Reprod 2014;3:268–274. DOI: https://doi.org/10.1016/S2305-0500(14)60038-5
Wiyasa I, Norahmawati E, Soehartono. Pengaruh Isoflavone Genistein dan Daidzein Ekstrak Tokbi (Pueraria lobata) strain Kangean terhadap Jumlah Osteoblas dan Osteoklas Rattus Novergiccus Wistar Hipoestrogenik. Maj Obs Ginekol Indones 2008;32:148–52.
Mibgels MJJ, Geels YP, Pijnenborg JMA, et al. Histipatologic assessement of the entire endometrium in asymtomatic women. Hum Pathol 2013;44:2293–2301. DOI: https://doi.org/10.1016/j.humpath.2013.05.011
Glazier, M Gina; Bowman MA. A review of teh evidence for the use of phytoestrogens as a replacement for traditional estrogen replacement therapy. Arch Intern Med 2001;1611:1161–72. DOI: https://doi.org/10.1001/archinte.161.9.1161
Ismiyati A, Wiyasa IW, Hidayati DY. Protective Effect of Vitamins C and E on Depot-Medroxyprogesterone Acetate-Induced Ovarian Oxidative Stress In Vivo. J Toxicol 2016;2016:3134105. DOI: https://doi.org/10.1155/2016/3134105
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