Culture conditions influence satellite cell activation and survival of single myofibers

Alessandra Renzini; Anna Benedetti; Marina Bouché; Leopoldo Silvestroni; Sergio Adamo; Viviana Moresi

doi:10.4081/ejtm.2018.7567

Original Articles Open access

Culture conditions influence satellite cell activation and survival of single myofibers

Authors

Alessandra Renzini – articles in this journal http://orcid.org/0000-0002-4265-7188 DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Anna Benedetti – articles in this journal http://orcid.org/0000-0003-2450-3705 DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Marina Bouché – articles in this journal http://orcid.org/0000-0002-0938-5360 DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Leopoldo Silvestroni – articles in this journal http://orcid.org/0000-0002-2197-7019 Department of Fundamental and Basic Sciences for Engineering, Sapienza University of Rome, Italy.
Sergio Adamo – articles in this journal Corresponding author Email http://orcid.org/0000-0002-1409-0452 DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Viviana Moresi – articles in this journal http://orcid.org/0000-0003-1912-0339 DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.

Published: 29 May 2018
Issue: Vol. 28 No. 2 (2018)
Section: Original Articles
DOI: 10.4081/ejtm.2018.7567

Abstract

Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved. This technique is an indispensable tool by which the muscle regeneration process can be recapitulated and studied in each specific phase, from satellite cell activation to proliferation, from differentiation to fusion. This study aims to clarify the effect of different culture conditions on single myofibers, their associated satellite cells, and the physiological behavior of the satellite cells upon long term culture. By direct observations of the cultures, we compared different experimental conditions and their effect on both satellite cell behavior and myofiber viability.

Keywords

Supporting Agencies

AFM Telethon, Sapienza University

Metrics

Citations and online attention from Dimensions, Altmetric and PlumX.

License

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.

Downloads

Article history

Received: 17 May 2018
Accepted: 24 May 2018
Published: 29 May 2018

Article statistics

1,861abstract views

974PDF

494APPENDIX

142HTML

Smart Citations (scite.ai)

How to Cite

1.

Renzini A, Benedetti A, Bouché M, Silvestroni L, Adamo S, Moresi V. Culture conditions influence satellite cell activation and survival of single myofibers. Eur J Transl Myol [Internet]. 2018 May 29 [cited 2026 Nov. 9];28(2). Available from: https://www.pagepressjournals.org/bam/article/view/7567

Download Citation

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.