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Effects of 3,5-diiodo-L-thyronine on the liver of high fat diet fed rats

Marco Giammanco, Stefania Aiello, Alessandra Casuccio, Maurizio La Guardia, Luca Cicero, Roberto Puleio, Irene Vazzana, Giovanni Tomasello, Giovanni Cassata, Gaetano Leto, Danila Di Majo
  • Stefania Aiello
    Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy
  • Alessandra Casuccio
    Department of Sciences for Health Promotion and Mother-Child Care, University of Palermo, Palermo, Italy
  • Maurizio La Guardia
    Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Palermo, Italy
  • Luca Cicero
    Institute for Experimental Veterinary Medicine of Sicily, Palermo, Italy
  • Roberto Puleio
    Institute for Experimental Veterinary Medicine of Sicily, Palermo, Italy
  • Irene Vazzana
    Institute for Experimental Veterinary Medicine of Sicily, Palermo, Italy
  • Giovanni Tomasello
    Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy
  • Giovanni Cassata
    Institute for Experimental Veterinary Medicine of Sicily, Palermo, Italy
  • Gaetano Leto
    Department of Sciences for Health Promotion and Mother-Child Care, University of Palermo, Palermo, Italy
  • Danila Di Majo
    Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy

Abstract

Experimental studies have highlighted that the administration of 3,5-diiodo-L-thyronine (T2) to rats fed diets rich in lipids induces a decrease of cholesterol and triglycerides plasma levels and body weight (BW) without inducing liver steatosis. On the basis of these observations we carried out some experimental in vivo studies to assess the effects of multiple high doses of T2 on the pituitary thyroid axis of rats fed diet rich in lipids. Fifteen male Wistar rats were divided into three groups of five animals each. The first group (N group) received standard diet, the second group was fed with a high fat diet (HFD group), while the third group (HFDT2 group) was additionally given T2 intraperitoneally at a dose level of 70 µg/100 g of BW three times a week up to four weeks. At the end of the treatment, blood sample from each animal was collected, centrifuged and the serum was stored at -20°C. The serum concentrations of thyroidstimulating hormone (TSH), triiodothyronine, thyroxine, adrenocorticotropic hormone, triglycerides, cholesterol, glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were then determined. In addition, liver of rats was examined by histology in order to assess the presence and degree of steatosis. The administration of T2 to rats fed with a high fat diet suppressed TSH secretion (P=0.013) while no steatosis was observed in the liver of these animals. Our data show that multiple administrations of high doses of T2 to rats fed diets rich in lipid inhibit TSH secretion and prevent the onset of liver steatosis in these animals.

Keywords

3,5-diiodo-L-thyronine; TSH; Thyroid hormone; Hepatic steatosis

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Submitted: 2015-12-04 12:34:04
Published: 2016-06-27 10:39:52
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Copyright (c) 2016 Marco Giammanco, Stefania Aiello, Alessandra Casuccio, Maurizio La Guardia, Luca Cicero, Roberto Puleio, Irene Vazzana, Giovanni Tomasello, Giovanni Cassata, Gaetano Leto, Danila Di Majo

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